Plant Plasma Membrane Proton Pump: One Protein with Multiple Functions

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 Dec 23

PMID 36552816

Authors

Adrianna Michalak

Anna Wdowikowska

Malgorzata Janicka

Affiliations

Soon will be listed here.

Abstract

In plants, the plasma membrane proton pump (PM H-ATPase) regulates numerous transport-dependent processes such as growth, development, basic physiology, and adaptation to environmental conditions. This review explores the multifunctionality of this enzyme in plant cells. The abundance of several PM H-ATPase isogenes and their pivotal role in energizing transport in plants have been connected to the phenomena of pleiotropy. The multifunctionality of PM H-ATPase is a focal point of numerous studies unraveling the molecular mechanisms of plant adaptation to adverse environmental conditions. Furthermore, PM H-ATPase is a key element in plant defense mechanisms against pathogen attack; however, it also functions as a target for pathogens that enable plant tissue invasion. Here, we provide an extensive review of the PM H-ATPase as a multitasking protein in plants. We focus on the results of recent studies concerning PM H-ATPase and its role in plant growth, physiology, and pathogenesis.

Citing Articles

Regulation of NO-Generating System Activity in Cucumber Root Response to Cold.

Reda M, Kabala K, Stanislawski J, Szczepski K, Janicka M Int J Mol Sci. 2025; 26(4).

PMID: 40004064 PMC: 11855582. DOI: 10.3390/ijms26041599.

Chromium "(VI)" phytoremediation using Azolla pinnata: effects on Vicia faba growth, physiology, cytogenetics, and gene expression profiling.

Soliman E, Moustafa K, Khamis M, Shedeed Z BMC Plant Biol. 2025; 25(1):160.

PMID: 39915722 PMC: 11874699. DOI: 10.1186/s12870-025-06115-7.

Identification and Analysis of the Plasma Membrane H-ATPase Gene Family in Cotton and Its Roles in Response to Salt Stress.

Cheng C, Zhang F, Li L, Ni Z Plants (Basel). 2025; 13(24.

PMID: 39771208 PMC: 11728463. DOI: 10.3390/plants13243510.

Antioxidant activity and comparative RNA-seq analysis support mitigating effects of an algae-based biostimulant on drought stress in tomato plants.

Cerruti P, Campobenedetto C, Montrucchio E, Agliassa C, Contartese V, Acquadro A Physiol Plant. 2024; 176(6):e70007.

PMID: 39703136 PMC: 11659800. DOI: 10.1111/ppl.70007.

Genome-Wide Association Study of Sweet Potato Storage Root Traits Using GWASpoly, a Gene Dosage-Sensitive Model.

Bowers R, Slonecki T, Olukolu B, Yencho G, Wadl P Int J Mol Sci. 2024; 25(21).

PMID: 39519288 PMC: 11546673. DOI: 10.3390/ijms252111727.

References

Mlodzinska E, Klobus G, Christensen M, Fuglsang A . The plasma membrane H(+) -ATPase AHA2 contributes to the root architecture in response to different nitrogen supply. Physiol Plant. 2014; 154(2):270-82. DOI: 10.1111/ppl.12305. View

Talke I, Hanikenne M, Kramer U . Zinc-dependent global transcriptional control, transcriptional deregulation, and higher gene copy number for genes in metal homeostasis of the hyperaccumulator Arabidopsis halleri. Plant Physiol. 2006; 142(1):148-67. PMC: 1557598. DOI: 10.1104/pp.105.076232. View

Schroeder J, Raschke K, Neher E . Voltage dependence of K channels in guard-cell protoplasts. Proc Natl Acad Sci U S A. 1987; 84(12):4108-12. PMC: 305032. DOI: 10.1073/pnas.84.12.4108. View

Bassil E, Blumwald E . The ins and outs of intracellular ion homeostasis: NHX-type cation/H(+) transporters. Curr Opin Plant Biol. 2014; 22:1-6. DOI: 10.1016/j.pbi.2014.08.002. View

Jahn T, Dietrich J, Andersen B, Leidvik B, Otter C, Briving C . Large scale expression, purification and 2D crystallization of recombinant plant plasma membrane H+-ATPase. J Mol Biol. 2001; 309(2):465-76. DOI: 10.1006/jmbi.2001.4688. View

Ritonga F, Chen S . Physiological and Molecular Mechanism Involved in Cold Stress Tolerance in Plants. Plants (Basel). 2020; 9(5). PMC: 7284489. DOI: 10.3390/plants9050560. View

Zhang B, Pasini R, Dan H, Joshi N, Zhao Y, Leustek T . Aberrant gene expression in the Arabidopsis SULTR1;2 mutants suggests a possible regulatory role for this sulfate transporter in response to sulfur nutrient status. Plant J. 2013; 77(2):185-97. DOI: 10.1111/tpj.12376. View

Palmgren M . PLANT PLASMA MEMBRANE H+-ATPases: Powerhouses for Nutrient Uptake. Annu Rev Plant Physiol Plant Mol Biol. 2001; 52:817-845. DOI: 10.1146/annurev.arplant.52.1.817. View

Rodriguez-Navarro A . Potassium transport in fungi and plants. Biochim Biophys Acta. 2000; 1469(1):1-30. DOI: 10.1016/s0304-4157(99)00013-1. View

10.

MOLLER J, Juul B, LE MAIRE M . Structural organization, ion transport, and energy transduction of P-type ATPases. Biochim Biophys Acta. 1996; 1286(1):1-51. DOI: 10.1016/0304-4157(95)00017-8. View

11.

Hippler F, Mattos-Jr D, Boaretto R, Williams L . Copper excess reduces nitrate uptake by Arabidopsis roots with specific effects on gene expression. J Plant Physiol. 2018; 228:158-165. PMC: 6090090. DOI: 10.1016/j.jplph.2018.06.005. View

12.

Goh C, Kinoshita T, Oku T, Shimazaki K . Inhibition of Blue Light-Dependent H+ Pumping by Abscisic Acid in Vicia Guard-Cell Protoplasts. Plant Physiol. 1996; 111(2):433-440. PMC: 157853. DOI: 10.1104/pp.111.2.433. View

13.

Kaundal A, Ramu V, Oh S, Lee S, Pant B, Lee H . GENERAL CONTROL NONREPRESSIBLE4 Degrades 14-3-3 and the RIN4 Complex to Regulate Stomatal Aperture with Implications on Nonhost Disease Resistance and Drought Tolerance. Plant Cell. 2017; 29(9):2233-2248. PMC: 5635975. DOI: 10.1105/tpc.17.00070. View

14.

Ram H, Kaur A, Gandass N, Singh S, Deshmukh R, Sonah H . Molecular characterization and expression dynamics of MTP genes under various spatio-temporal stages and metal stress conditions in rice. PLoS One. 2019; 14(5):e0217360. PMC: 6538162. DOI: 10.1371/journal.pone.0217360. View

15.

Rayle D, Cleland R . The Acid Growth Theory of auxin-induced cell elongation is alive and well. Plant Physiol. 1992; 99(4):1271-4. PMC: 1080619. DOI: 10.1104/pp.99.4.1271. View

16.

Baxter I, Tchieu J, Sussman M, Boutry M, Palmgren M, Gribskov M . Genomic comparison of P-type ATPase ion pumps in Arabidopsis and rice. Plant Physiol. 2003; 132(2):618-28. PMC: 167002. DOI: 10.1104/pp.103.021923. View

17.

Takahashi K, Hayashi K, Kinoshita T . Auxin activates the plasma membrane H+-ATPase by phosphorylation during hypocotyl elongation in Arabidopsis. Plant Physiol. 2012; 159(2):632-41. PMC: 3375930. DOI: 10.1104/pp.112.196428. View

18.

Janicka-Russak M, Kabala K, Wdowikowska A, Klobus G . Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Plant Physiol. 2013; 170(10):915-22. DOI: 10.1016/j.jplph.2013.02.002. View

19.

Isayenkov S, Dabravolski S, Pan T, Shabala S . Phylogenetic Diversity and Physiological Roles of Plant Monovalent Cation/H Antiporters. Front Plant Sci. 2020; 11:573564. PMC: 7573149. DOI: 10.3389/fpls.2020.573564. View

20.

Anschutz U, Becker D, Shabala S . Going beyond nutrition: regulation of potassium homoeostasis as a common denominator of plant adaptive responses to environment. J Plant Physiol. 2014; 171(9):670-87. DOI: 10.1016/j.jplph.2014.01.009. View