» Articles » PMID: 36552669

Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model

Abstract

Cigarette smoking-induced oxidative stress has harmful effects on bone metabolism. Maqui berry extract (MBE) and ginseng extract (GE) are two naturally occurring antioxidants that have been shown to reduce oxidative stress. By using an osteoblast and osteoclast three-dimensional co-culture system, we investigated the effects of MBE and GE on bone cells exposed to cigarette smoke extract (CSE). The cell viability and function of the co-culture system were measured on day 14. Markers of bone cell differentiation and oxidative stress were evaluated at gene and protein levels on day 7. The results showed that exposure to CSE induced osteoporotic-like alterations in the co-culture system, while 1.5 µg/mL MBE and 50 µg/mL GE improved CSE-impaired osteoblast function and decreased CSE-induced osteoclast function. The molecular mechanism of MBE and GE in preventing CSE-induced bone cell damage is linked with the inhibition of the NF-κB signaling pathway and the activation of the Nrf2 signaling pathway. Therefore, MBE and GE can reduce CSE-induced detrimental effects on bone cells and, thus, prevent smoking-induced alterations in bone cell homeostasis. These two antioxidants are thus suitable supplements to support bone regeneration in smokers.

Citing Articles

Impact of Particle Size and Sintering Temperature on Calcium Phosphate Gyroid Structure Scaffolds for Bone Tissue Engineering.

Aspera-Werz R, Chen G, Schilonka L, Bouakaz I, Bronne C, Cobraiville E J Funct Biomater. 2024; 15(12.

PMID: 39728155 PMC: 11727752. DOI: 10.3390/jfb15120355.


Establishment of a human 3D in vitro liver-bone model as a potential system for drug toxicity screening.

Chen G, Xin Y, Hammour M, Braun B, Ehnert S, Springer F Arch Toxicol. 2024; 99(1):333-356.

PMID: 39503877 PMC: 11742461. DOI: 10.1007/s00204-024-03899-9.


Aggregation of human osteoblasts unlocks self-reliant differentiation and constitutes a microenvironment for 3D-co-cultivation with other bone marrow cells.

Marozin S, Simon-Nobbe B, Huth A, Beyerer E, Weber L, Nussler A Sci Rep. 2024; 14(1):10345.

PMID: 38710795 PMC: 11074281. DOI: 10.1038/s41598-024-60986-8.


A Review of the Functional Characteristics and Applications of (Maqui Berry), in the Food Industry.

Garcia-Milla P, Penalver R, Nieto G Foods. 2024; 13(6).

PMID: 38540828 PMC: 10969556. DOI: 10.3390/foods13060838.


Antioxidant Properties of Maqui Berry Extract ( (Mol.) Stuntz) and Its Potential Photoprotective Role on Human Skin Fibroblasts.

Wacewicz-Muczynska M, Moskwa J, Puscion-Jakubik A, Naliwajko S, Niczyporuk M, Socha K Molecules. 2023; 28(23).

PMID: 38067532 PMC: 10708373. DOI: 10.3390/molecules28237802.


References
1.
Aspera-Werz R, Chen T, Ehnert S, Zhu S, Frohlich T, Nussler A . Cigarette Smoke Induces the Risk of Metabolic Bone Diseases: Transforming Growth Factor Beta Signaling Impairment via Dysfunctional Primary Cilia Affects Migration, Proliferation, and Differentiation of Human Mesenchymal Stem Cells. Int J Mol Sci. 2019; 20(12). PMC: 6628373. DOI: 10.3390/ijms20122915. View

2.
Nagaoka M, Maeda T, Chatani M, Handa K, Yamakawa T, Kiyohara S . A Delphinidin-Enriched Maqui Berry Extract Improves Bone Metabolism and Protects against Bone Loss in Osteopenic Mouse Models. Antioxidants (Basel). 2019; 8(9). PMC: 6769591. DOI: 10.3390/antiox8090386. View

3.
Byon C, Javed A, Dai Q, Kappes J, Clemens T, Darley-Usmar V . Oxidative stress induces vascular calcification through modulation of the osteogenic transcription factor Runx2 by AKT signaling. J Biol Chem. 2008; 283(22):15319-27. PMC: 2397455. DOI: 10.1074/jbc.M800021200. View

4.
Heher P, Ganassi M, Weidinger A, Engquist E, Pruller J, Nguyen T . Interplay between mitochondrial reactive oxygen species, oxidative stress and hypoxic adaptation in facioscapulohumeral muscular dystrophy: Metabolic stress as potential therapeutic target. Redox Biol. 2022; 51:102251. PMC: 8899416. DOI: 10.1016/j.redox.2022.102251. View

5.
Chen E, Lin Y . Using synthetic peptides and recombinant collagen to understand DDR-collagen interactions. Biochim Biophys Acta Mol Cell Res. 2019; 1866(11):118458. DOI: 10.1016/j.bbamcr.2019.03.005. View