Affects the Daily Weight Gain of the F2 Population and Regulates Myogenic Cell Proliferation and Differentiation in Chickens

Overview

Journal Animals (Basel)

Date 2022 Dec 23

PMID 36552455

Authors

Tuanhui Ren

Wujian Lin

Xiuxian Yang

Zihao Zhang

Shizi He

Wangyu Li

Zhuanjian Li

Xiquan Zhang

Affiliations

Soon will be listed here.

Abstract

Molecular breeding can accelerate the process of animal breeding and improve the breeding efficiency. To date, many Indel molecular markers have been identified in livestock and poultry, but how Indels affect economic traits is not well understood. For molecular breeding, it is crucial to reveal the mechanism of action of Indels and to provide more accurate information. The purpose of this study was to investigate how the 52/224-bp multiallelic Indels of the chicken promoter area affect the daily weight gain of chickens and the potential regulatory mechanism of the gene. The analysis was conducted by association analysis, qPCR, dual-fluorescence assay and Western blotting. The results showed that Indels in the promoter region were significantly associated with the daily weight gain in chickens and that expression showed a decreasing trend in embryonic breast muscle tissues. Furthermore, expression was significantly higher in breast muscle tissues of the AC genotype than in those of the AB and BB genotypes. Based on the transcriptional activity results, the pGL3-C vector produced more luciferase activity than pGL3-A and pGL3-B. In addition, overexpression of promoted chicken primary myoblast (CPM) proliferation and inhibited differentiation. The results of this study suggest that Indels in the promoter region of the gene may regulate the proliferation and differentiation of CPMs by affecting the expression of , which ultimately affects the growth rate of chickens. These Indels have important value for the molecular breeding of chickens, and can be used as a candidate gene to regulate and improve chicken growth and development.

Citing Articles

Regulatory Effects of 198-bp Structural Variants in the Promoter Region on Adipogenesis in Chickens.

Li W, Xu M, Zhang Z, Liang J, Fu R, Lin W Int J Mol Sci. 2024; 25(13).

PMID: 39000259 PMC: 11241197. DOI: 10.3390/ijms25137155.

References

Gaya L, Ferraz J, Rezende F, Mourao G, Mattos E, Eler J . Heritability and genetic correlation estimates for performance and carcass and body composition traits in a male broiler line. Poult Sci. 2006; 85(5):837-43. DOI: 10.1093/ps/85.5.837. View

Jin H, Yoo H, Kim Y, Lee J, Lee Y, Kwon S . Unveiling genetic variants for age-related sarcopenia by conducting a genome-wide association study on Korean cohorts. Sci Rep. 2022; 12(1):3501. PMC: 8894365. DOI: 10.1038/s41598-022-07567-9. View

Fontanesi L, Schiavo G, Galimberti G, Calo D, Russo V . A genomewide association study for average daily gain in Italian Large White pigs. J Anim Sci. 2014; 92(4):1385-94. DOI: 10.2527/jas.2013-7059. View

Li W, Liu D, Tang S, Li D, Han R, Tian Y . A multiallelic indel in the promoter region of the Cyclin-dependent kinase inhibitor 3 gene is significantly associated with body weight and carcass traits in chickens. Poult Sci. 2018; 98(2):556-565. DOI: 10.3382/ps/pey404. View

Mills R, Luttig C, Larkins C, Beauchamp A, Tsui C, Pittard W . An initial map of insertion and deletion (INDEL) variation in the human genome. Genome Res. 2006; 16(9):1182-90. PMC: 1557762. DOI: 10.1101/gr.4565806. View

Williams M, Almen M, Fredriksson R, Schioth H . What model organisms and interactomics can reveal about the genetics of human obesity. Cell Mol Life Sci. 2012; 69(22):3819-34. PMC: 11114734. DOI: 10.1007/s00018-012-1022-5. View

Lin W, Ren T, Li W, Liu M, He D, Liang S . Novel 61-bp Indel of Is Associated With Fat and Hatching Weight Traits in Chickens. Front Genet. 2021; 12:672888. PMC: 8280519. DOI: 10.3389/fgene.2021.672888. View

Han R, Wang X, Wang X, Guo Y, Li D, Li G . Chicken ZNF764L gene: mRNA expression profile, alternative splicing analysis and association analysis between first exon indel mutation and economic traits. Gene. 2019; 695:92-98. DOI: 10.1016/j.gene.2019.02.010. View

Xu Z, Ji C, Zhang Y, Zhang Z, Nie Q, Xu J . Combination analysis of genome-wide association and transcriptome sequencing of residual feed intake in quality chickens. BMC Genomics. 2016; 17:594. PMC: 4979145. DOI: 10.1186/s12864-016-2861-5. View

10.

Hitomi T, Matsuzaki Y, Yasuda S, Kawanaka M, Yogosawa S, Koyama M . Oct-1 is involved in the transcriptional repression of the p15(INK4b) gene. FEBS Lett. 2007; 581(6):1087-92. DOI: 10.1016/j.febslet.2007.01.092. View

11.

Hui Y, Zhang Y, Wang K, Pan C, Chen H, Qu L . Goat DNMT3B: An indel mutation detection, association analysis with litter size and mRNA expression in gonads. Theriogenology. 2020; 147:108-115. DOI: 10.1016/j.theriogenology.2020.02.025. View

12.

Chen B, Guo L, Chen X, El-Senousey H, Ma M, Jebessa E . Cellular function of chicken FOXO3 and its associations with chicken growth. Poult Sci. 2019; 98(10):5109-5117. DOI: 10.3382/ps/pez397. View

13.

Bresser K, Logtenberg M, Toebes M, Proost N, Sprengers J, Siteur B . QPCTL regulates macrophage and monocyte abundance and inflammatory signatures in the tumor microenvironment. Oncoimmunology. 2022; 11(1):2049486. PMC: 8932921. DOI: 10.1080/2162402X.2022.2049486. View

14.

Xu Y, Shi T, Zhou Y, Liu M, Klaus S, Lan X . A novel PAX7 10-bp indel variant modulates promoter activity, gene expression and contributes to different phenotypes of Chinese cattle. Sci Rep. 2018; 8(1):1724. PMC: 5789009. DOI: 10.1038/s41598-018-20177-8. View

15.

Madlala T, Okpeku M, Adeleke M . Understanding the interactions between Eimeria infection and gut microbiota, towards the control of chicken coccidiosis: a review. Parasite. 2021; 28:48. PMC: 8171251. DOI: 10.1051/parasite/2021047. View

16.

Li Z, Cai B, Abdalla B, Zhu X, Zheng M, Han P . LncIRS1 controls muscle atrophy via sponging miR-15 family to activate IGF1-PI3K/AKT pathway. J Cachexia Sarcopenia Muscle. 2019; 10(2):391-410. PMC: 6463472. DOI: 10.1002/jcsm.12374. View

17.

Dorajoo R, Blakemore A, Sim X, Ong R, Ng D, Seielstad M . Replication of 13 obesity loci among Singaporean Chinese, Malay and Asian-Indian populations. Int J Obes (Lond). 2011; 36(1):159-63. DOI: 10.1038/ijo.2011.86. View

18.

Ren T, Zhang Z, Fu R, Yang Y, Li W, Liang J . A 51 bp indel polymorphism within the PTH1R gene is significantly associated with chicken growth and carcass traits. Anim Genet. 2020; 51(4):568-578. DOI: 10.1111/age.12942. View

19.

Wei C, Niu Y, Chen B, Qin P, Wang Y, Hou D . Genetic effect of an InDel in the promoter region of the NUDT15 and its effect on myoblast proliferation in chickens. BMC Genomics. 2022; 23(1):138. PMC: 8848950. DOI: 10.1186/s12864-022-08362-6. View

20.

Luo W, Wu H, Ye Y, Li Z, Hao S, Kong L . The transient expression of miR-203 and its inhibiting effects on skeletal muscle cell proliferation and differentiation. Cell Death Dis. 2014; 5:e1347. PMC: 4123083. DOI: 10.1038/cddis.2014.289. View