Acyl-Lipid Δ-Desaturase May Act As a First FAD in Cyanobacteria

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2022 Dec 23

PMID 36551223

Authors

Alexander Y Starikov

Roman A Sidorov

Sergei V Goriainov

Dmitry A Los

Affiliations

Soon will be listed here.

Abstract

Fatty acid desaturases (FADs) play important roles in various metabolic and adaptive pathways in all living organisms. They represent a superfamily of oxygenases that introduce double bonds into the acyl chains of fatty acids (FAs). These enzymes are highly specific to the length of the carbon chain, position of double bonds formation, etc. The modes by which FADs "count" the position of the double bond formation may differ. In cyanobacteria, the first double bond is formed between 9th and 10th carbons (position Δ), counting from the carboxylic end of an FA. Other FADs that produce polyunsaturated FAs may introduce double bonds counting from the carboxyl (Δ) or methyl (ω) terminus, or from a pre-existing double bond towards carboxyl or methyl terminus of an FA chain. Here, we expressed the gene for the Δ6-FAD from sp. PCC 6803 in PCC 7942 (which is capable of synthesizing only monoenoic FAs desaturated mainly at position Δ) and observed the appearance of unusual monoenoic FAs desaturated at position Δ, as well as Δ dienoic FAs. Exogenously added -10-heptadecenoic acid (17:1Δ) was converted into -6,10-heptadecadienoic (17:2Δ). These data demonstrate the ability of Δ6-FAD to introduce the first double bond into the unsaturated substrates and suggests that it "counts" from the carboxyl end, irrespective of the absence or presence of a previous double bond in an FA chain.

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