Immunostimulatory Effects of a Subcritical Water Extract of

Overview

Journal Biomed Rep

Specialty Biochemistry

Date 2022 Dec 22

PMID 36544853

Authors

Koji Hattori

Hiroshi Takagi

Yuichiro Ogata

Takaaki Yamada

Hiroki Horiba

Kousuke Fukata

Tsutomu Sakaida

Youichi Yashiro

Seiji Hasegawa

Hiroyuki Tanaka

Affiliations

Soon will be listed here.

Abstract

, a medicinal mushroom with various physiological activities, has been extensively investigated regarding its effectiveness. The aim of the present study was to examine the effects of a subcritical water extract of (SWEG) on the immune system. The use of subcritical water with a higher temperature and pressure than hot water allows efficient elution of components from natural products. As an evaluation of the effectiveness of SWEG, a cell proliferation and a cell differentiation test were carried out using A-6 cells, a model of hematopoietic stem cells. Furthermore, an oral administration test in mice was conducted to examine the effects of SWEG on the number and function of immune cells. As a result, SWEG was revealed to promote both self-renewal and differentiation into immune cells such as T cells and natural killer (NK) cells in experiments with A-6 cells. These results were not obtained in experiments using hot water extract of and . The oral administration test in mice demonstrated that SWEG increased hematopoietic precursor cells, immature B cells, and NK cells in the bone marrow, and T cells in the thymus. In addition, SWEG enhanced the immune functions in the spleen by promoting granzyme B expression and NK cell activity. SWEG was demonstrated to be a food material that acts on HSCs and regulates immunity .

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