The Graham and Karnovsky Horseradish Peroxidase Ultrastructural Method: A Premier JHC Citation Classic
Overview
Overview
Journal
J Histochem Cytochem
Publisher
Sage Publications
Specialty
Biochemistry
Date
2022 Dec 21
PMID
36541711
Authors
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
This commentary briefly reviews the background for the development of the horseradish peroxidase-diaminobenzidine tetrahydrochloride histochemical method originally described by Graham and Karnovsky in their citation classic, reprinted in full in this issue of . Some of the method's subsequent applications, including its use as a macromolecular tracer for kidney glomerular permeability and use in immunoelectron microscopy and other immunoassays, are also discussed.
References
1.
Nakane P, Kawaoi A
. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974; 22(12):1084-91.
DOI: 10.1177/22.12.1084.
View
2.
Montinaro V, Giliberti M, Villani C, Montinaro A
. Citation classics: ranking of the top 100 most cited articles in nephrology. Clin Kidney J. 2019; 12(1):6-18.
PMC: 6366133.
DOI: 10.1093/ckj/sfy033.
View
3.
GRAHAM Jr R, Karnovsky M
. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966; 14(4):291-302.
DOI: 10.1177/14.4.291.
View
4.
RYAN G, Karnovsky M
. Distribution of endogenous albumin in the rat glomerulus: role of hemodynamic factors in glomerular barrier function. Kidney Int. 1976; 9(1):36-45.
DOI: 10.1038/ki.1976.5.
View
5.
GRAHAM Jr R, Karnovsky M
. Glomerular permeability. Ultrastructural cytochemical studies using peroxidases as protein tracers. J Exp Med. 1966; 124(6):1123-34.
PMC: 2138332.
DOI: 10.1084/jem.124.6.1123.
View