Protective Effects of the Fructooligosaccharide on the Growth Performance, Biochemical Indexes, and Intestinal Morphology of Blunt Snout Bream (Megalobrama Amblycephala) Infected by Aeromonas Hydrophila

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2022 Dec 20

PMID 36538149

Authors

Dongxue Jiang

Shengnan Li

Yuexia Liang

Junqi Ma

Bingke Wang

Chunnuan Zhang

Affiliations

Soon will be listed here.

Abstract

The purpose of the study was to investigate the effects of dietary fructooligosaccharide (FOS) on growth performance, biochemical indexes, intestinal morphology, and growth-related gene expression of blunt snout bream (Megalobrama amblycephala) infected by Aeromonas hydrophila (AH). Two hundred twenty-five healthy blunt snout bream with an initial body weight of 38.41 ± 0.88 g were randomly divided into five groups with three replicates: control (basal diet), model (AH + basal diet), SFOS (AH + 2 g/kg FOS), MFOS (AH + 4 g/kg FOS), LFOS (AH + 6 g/kg FOS). After 9 weeks of feeding, the results showed that the FOS-added diet abrogated AH-induced retardation, hemorrhage, and inflammatory infiltration. FOS supplementation enhanced the growth performance degradation caused by AH, and the highest growth performance was observed at MFOS. Meanwhile, the addition of FOS to feed improved the blood immunity reduced by AH. In expansion, the mucosal epithelium of intestinal villi exfoliated, exposing the lamina propria, and a few villi were genuinely harmed in the model group. Fish fed with MFOS ameliorated the damaged intestine, evidenced by well-preserved intestine architecture. Furthermore, the model group downregulated the expression of growth-related genes (growth hormone receptor (GHR), insulin-like growth factor 1 (IGF-1)). Fish fed with 2 g/kg or 4 g/kg FOS upregulated the genes specified above expressions in the liver compared with the model group. In conclusion, the results mentioned above suggested that the dietary FOS could relieve the pressure to elevate the immune damage and intestine injury induced by AH and enhance the hepatic expression of IGF-1 and GHR.

Citing Articles

Dietary Supplementation with R-(+)-Limonene Improves Growth, Metabolism, Stress, and Antioxidant Responses of Silver Catfish Uninfected and Infected with .

da Silva E, Finamor I, Bressan C, Schoenau W, de Souza Vencato M, Pavanato M Animals (Basel). 2023; 13(21).

PMID: 37958062 PMC: 10650795. DOI: 10.3390/ani13213307.

Effects of maggot antimicrobial peptides on growth performance, immune function, and cecal flora of yellow-feathered broilers.

Gao S, Zhang Q, Liu C, Shen H, Wang J Front Vet Sci. 2023; 10:1156964.

PMID: 37559887 PMC: 10407657. DOI: 10.3389/fvets.2023.1156964.

References

Anson M . THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBIN. J Gen Physiol. 2009; 22(1):79-89. PMC: 2213732. DOI: 10.1085/jgp.22.1.79. View

Beckman B . Perspectives on concordant and discordant relations between insulin-like growth factor 1 (IGF1) and growth in fishes. Gen Comp Endocrinol. 2010; 170(2):233-52. DOI: 10.1016/j.ygcen.2010.08.009. View

Carbone D, Faggio C . Importance of prebiotics in aquaculture as immunostimulants. Effects on immune system of Sparus aurata and Dicentrarchus labrax. Fish Shellfish Immunol. 2016; 54:172-8. DOI: 10.1016/j.fsi.2016.04.011. View

Dawood M, Basuini M, Zaineldin A, Yilmaz S, Hasan M, Ahmadifar E . Antiparasitic and Antibacterial Functionality of Essential Oils: An Alternative Approach for Sustainable Aquaculture. Pathogens. 2021; 10(2). PMC: 7914417. DOI: 10.3390/pathogens10020185. View

Done H, Venkatesan A, Halden R . Does the Recent Growth of Aquaculture Create Antibiotic Resistance Threats Different from those Associated with Land Animal Production in Agriculture?. AAPS J. 2015; 17(3):513-24. PMC: 4406955. DOI: 10.1208/s12248-015-9722-z. View

Dong Y, Li L, Espe M, Lu K, Rahimnejad S . Hydroxytyrosol Attenuates Hepatic Fat Accumulation via Activating Mitochondrial Biogenesis and Autophagy through the AMPK Pathway. J Agric Food Chem. 2020; 68(35):9377-9386. DOI: 10.1021/acs.jafc.0c03310. View

Dong Y, Yu M, Wu Y, Xia T, Wang L, Song K . Hydroxytyrosol Promotes the Mitochondrial Function through Activating Mitophagy. Antioxidants (Basel). 2022; 11(5). PMC: 9138034. DOI: 10.3390/antiox11050893. View

Guerrero-Zarate R, Alvarez-Gonzalez C, Olvera-Novoa M, Perales-Garcia N, Frias-Quintana C, Martinez-Garcia R . Partial characterization of digestive proteases in tropical gar Atractosteus tropicus juveniles. Fish Physiol Biochem. 2014; 40(4):1021-9. DOI: 10.1007/s10695-013-9902-7. View

Huerta B, Arenas A, Carrasco L, Maldonado A, Tarradas C, Carbonero A . Comparison of diagnostic techniques for porcine proliferative enteropathy (Lawsonia intracellularis infection). J Comp Pathol. 2003; 129(2-3):179-85. DOI: 10.1016/s0021-9975(03)00030-6. View

10.

Jiang J, Zhao M, Lin F, Yang L, Zhou X . Effect of conjugated linoleic acid on Cyprinus carpio var. Jian regarding growth, immunity, and disease resistance to Aeromonas hydrophila. Lipids. 2010; 45(6):531-6. DOI: 10.1007/s11745-010-3425-0. View

11.

Kojima M, Kangawa K . Ghrelin: structure and function. Physiol Rev. 2005; 85(2):495-522. DOI: 10.1152/physrev.00012.2004. View

12.

Liu Y, Xu Q, Wang Y, Liang T, Li X, Wang D . Necroptosis is active and contributes to intestinal injury in a piglet model with lipopolysaccharide challenge. Cell Death Dis. 2021; 12(1):62. PMC: 7801412. DOI: 10.1038/s41419-020-03365-1. View

13.

Olesen M . Efficacy, safety, and tolerability of fructooligosaccharides in the treatment of irritable bowel syndrome. Am J Clin Nutr. 2000; 72(6):1570-5. DOI: 10.1093/ajcn/72.6.1570. View

14.

Ramadan H, Ibrahim N, Samir M, Abd El-Moaty A, Gad T . Aeromonas hydrophila from marketed mullet (Mugil cephalus) in Egypt: PCR characterization of β-lactam resistance and virulence genes. J Appl Microbiol. 2018; 124(6):1629-1637. DOI: 10.1111/jam.13734. View

15.

Reinecke M . Influences of the environment on the endocrine and paracrine fish growth hormone-insulin-like growth factor-I system. J Fish Biol. 2010; 76(6):1233-54. DOI: 10.1111/j.1095-8649.2010.02605.x. View

16.

Soleimani N, Hoseinifar S, Merrifield D, Barati M, Abadi Z . Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol. 2011; 32(2):316-21. DOI: 10.1016/j.fsi.2011.11.023. View

17.

Taylor S, Ramsamooj S, Liang R, Katti A, Pozovskiy R, Vasan N . Dietary fructose improves intestinal cell survival and nutrient absorption. Nature. 2021; 597(7875):263-267. PMC: 8686685. DOI: 10.1038/s41586-021-03827-2. View

18.

Wenger M, Shved N, Akgul G, Caelers A, Casanova A, Segner H . Developmental oestrogen exposure differentially modulates IGF-I and TNF-α expression levels in immune organs of Yersinia ruckeri-challenged young adult rainbow trout (Oncorhynchus mykiss). Gen Comp Endocrinol. 2014; 205:168-75. DOI: 10.1016/j.ygcen.2014.05.022. View

19.

Yang C, Kong J, Wang Q, Liu Q, Tian Y, Luo K . Heterosis of haemolymph analytes of two geographic populations in Chinese shrimp Fenneropenaeus chinensis. Fish Shellfish Immunol. 2007; 23(1):62-70. DOI: 10.1016/j.fsi.2006.09.005. View

20.

Zhang C, Li X, Xu W, Jiang G, Lu K, Wang L . Combined effects of dietary fructooligosaccharide and Bacillus licheniformis on innate immunity, antioxidant capability and disease resistance of triangular bream (Megalobrama terminalis). Fish Shellfish Immunol. 2013; 35(5):1380-6. DOI: 10.1016/j.fsi.2013.07.047. View