Physiological and Comparative Proteomic Characterization of Gen. Nov., Sp. Nov., a Novel Mesophilic, Sulfur-disproportionating Chemolithoautotroph from a Deep-sea Hydrothermal Vent

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Dec 19

PMID 36532468

Authors

Yurina Hashimoto

Shigeru Shimamura

Akihiro Tame

Shigeki Sawayama

Junichi Miyazaki

Ken Takai

Satoshi Nakagawa

Affiliations

Soon will be listed here.

Abstract

In deep-sea hydrothermal environments, inorganic sulfur compounds are important energy substrates for sulfur-oxidizing, -reducing, and -disproportionating microorganisms. Among these, sulfur-disproportionating bacteria have been poorly understood in terms of ecophysiology and phylogenetic diversity. Here, we isolated and characterized a novel mesophilic, strictly chemolithoautotrophic, diazotrophic sulfur-disproportionating bacterium, designated strain GF1, from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Izu-Bonin Arc, Japan. Strain GF1 disproportionated elemental sulfur, thiosulfate, and tetrathionate in the presence of ferrihydrite. The isolate also grew by respiratory hydrogen oxidation coupled to sulfate reduction. Phylogenetic and physiological analyses support that strain GF1 represents the type strain of a new genus and species in the family , for which the name gen. nov. sp. nov. is proposed. Proteomic analysis revealed that proteins related to tetrathionate reductase were specifically and abundantly produced when grown thiosulfate disproportionation. In addition, several proteins possibly involved in thiosulfate disproportionation, including those encoded by the YTD gene cluster, were also found. The overall findings pointed to a possible diversity of sulfur-disproportionating bacteria in hydrothermal systems and provided a refined picture of microbial sulfur disproportionation.

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