Milk Protection Against Experimental Ulcerogenesis in Rats

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 1987 Oct 1

PMID 3652898

Citations 8

Authors

E J Dial

L M Lichtenberger

Affiliations

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Abstract

The antiulcer activity in pasteurized/homogenized bovine milk and a lipid extract of this milk was tested in an attempt to isolate and identify the active component. Using 0.6 N HCl as a damaging agent in pylorus-ligated rats, the protective property of milk appeared to be related to its phospholipid content and not its protein constituents. With intact (non-pylorus-ligated) rats, milk had demonstrable protective activity against 0.6 N HCl, as well as 100% ethanol and 160 mM taurocholic acid. The increasing phospholipid concentrations in skim, whole, and buttermilk paralleled their antiulcer efficacy. A lipid extract of whole milk showed significant protection against 0.6 N HCl when given alone or following indomethacin treatment. Measurements of the contact angle (hydrophobicity) of the gastric surface showed that it was maintained near control levels in the presence of 0.6 N HCl, if rats were first pretreated with milk. These results are consistent with the possibility that surface-active lipids in dairy milk, such as phospholipids, may account for a significant portion of milk's antiulcer activity by maintaining the hydrophobicity of the luminal surface of the gastric mucosa in the presence of a damaging agent.

Citing Articles

Dietary Phytosterols Protective Against Peptic Ulceration.

Tovey F, Capanoglu D, Langley G, Herniman J, Bor S, Ozutemiz O Gastroenterology Res. 2016; 4(4):149-156.

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Helicobacter pylori: the primary cause of duodenal ulceration or a secondary infection?.

Hobsley M, Tovey F World J Gastroenterol. 2002; 7(2):149-51.

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Age and Helicobacter pylori decrease gastric mucosal surface hydrophobicity independently.

Hackelsberger A, Platzer U, Nilius M, Schultze V, Gunther T, Dominguez-Munoz J Gut. 1998; 43(4):465-9.

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Inhibitory effect of polyunsaturated fatty acids on the growth of Helicobacter pylori: a possible explanation of the effect of diet on peptic ulceration.

Thompson L, Cockayne A, Spiller R Gut. 1994; 35(11):1557-61.

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Gastroprotection by dairy foods against stress-induced ulcerogenesis in rats.

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PMID: 7587804 DOI: 10.1007/BF02063227.

References

Hills B, Butler B, Lichtenberger L . Gastric mucosal barrier: hydrophobic lining to the lumen of the stomach. Am J Physiol. 1983; 244(5):G561-8. DOI: 10.1152/ajpgi.1983.244.5.G561. View

Robert A, NEZAMIS J, Lancaster C, Hanchar A . Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology. 1979; 77(3):433-43. View

Paffenbarger Jr R, Wing A, HYDE R . Chronic disease in former college students; 13. Early precursors of peptic ulcer. Am J Epidemiol. 1974; 100(4):307-15. DOI: 10.1093/oxfordjournals.aje.a112039. View

Spiro H . Is milk all that bad for the ulcer patient?. J Clin Gastroenterol. 1981; 3(3):219-20. DOI: 10.1097/00004836-198109000-00003. View

Chaudhury T, Robert A . Prevention by mild irritants of gastric necrosis produced in rats by sodium taurocholate. Dig Dis Sci. 1980; 25(11):830-6. DOI: 10.1007/BF01338524. View

WATSON L, Abston S . Prevention of upper gastrointestinal hemorrhage in 582 burned children. Am J Surg. 1976; 132(6):790-3. DOI: 10.1016/0002-9610(76)90460-8. View

Rouser G, Fkeischer S, Yamamoto A . Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids. 1970; 5(5):494-6. DOI: 10.1007/BF02531316. View

Dial E, Lichtenberger L . A role for milk phospholipids in protection against gastric acid. Studies in adult and suckling rats. Gastroenterology. 1984; 87(2):379-85. View

Robert A, NEZAMIS J, Lancaster C, Davis J, Field S, Hanchar A . Mild irritants prevent gastric necrosis through "adaptive cytoprotection" mediated by prostaglandins. Am J Physiol. 1983; 245(1):G113-21. DOI: 10.1152/ajpgi.1983.245.1.G113. View

10.

Materia A, Jaffe B, Money S, Rossi P, De Marco M, Basso N . Prostaglandins in commercial milk preparations. Their effect in the prevention of stress-induced gastric ulcer. Arch Surg. 1984; 119(3):290-2. DOI: 10.1001/archsurg.1984.01390150032008. View

11.

Lichtenberger L, Graziani L, Dial E, Butler B, Hills B . Role of surface-active phospholipids in gastric cytoprotection. Science. 1983; 219(4590):1327-9. DOI: 10.1126/science.6828859. View

12.

Mason R, Nellenbogen J, Clements J . Isolation of disaturated phosphatidylcholine with osmium tetroxide. J Lipid Res. 1976; 17(3):281-4. View

13.

VAHOUNY G, Tombes R, Cassidy M, Kritchevsky D, Gallo L . Dietary fibres. VI: Binding of fatty acids and monolein from mixed micelles containing bile salts and lecithin. Proc Soc Exp Biol Med. 1981; 166(1):12-6. DOI: 10.3181/00379727-166-41016. View

14.

Dial E, Lichtenberger L . Development of gastric mucosal protection against acid in the rat. Role of corticosteroids and prostaglandins. Gastroenterology. 1986; 91(2):318-25. DOI: 10.1016/0016-5085(86)90563-9. View

15.

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

SANDWEISS D . The Sippy treatment for peptic ulcer-fifty years later. Am J Dig Dis. 1961; 6:929-37. DOI: 10.1007/BF02231424. View