A Novel Ambigrammatic Mycovirus, PsV5, Works Hand in Glove with Wheat Stripe Rust Fungus to Facilitate Infection

Overview

Journal Plant Commun

Specialty Biology

Date 2022 Dec 17

PMID 36527233

Authors

Yanhui Zhang

Xiaofei Liang

Mengxin Zhao

Tuo Qi

Hualong Guo

Jing Zhao

Jie Zhao

Gangming Zhan

Zhensheng Kang

Li Zheng

Affiliations

Soon will be listed here.

Abstract

Here we describe a novel narnavirus, Puccinia striiformis virus 5 (PsV5), from the devastating wheat stripe rust fungus P. striiformis f. sp. tritici (Pst). The genome of PsV5 contains two predicted open reading frames (ORFs) that largely overlap on reverse strands: an RNA-dependent RNA polymerase (RdRp) and a reverse-frame ORF (rORF) with unknown function. Protein translations of both ORFs were demonstrated by immune technology. Transgenic wheat lines overexpressing PsV5 (RdRp-rORF), RdRp ORF, or rORF were more susceptible to Pst infection, whereas PsV5-RNA interference (RNAi) lines were more resistant. Overexpression of PsV5 (RdRp-rORF), RdRp ORF, or rORF in Fusarium graminearum also boosted fungal virulence. We thus report a novel ambigrammatic mycovirus that promotes the virulence of its fungal host. The results are a significant addition to our understanding of virosphere diversity and offer insights for sustainable wheat rust disease control.

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