Effect of on Beta Catenin and Wnt MRNA Expression in Human Colon Cancer (HT-29) Cells in Vitro

Overview

Journal Bioinformation

Specialty Biology

Date 2022 Dec 15

PMID 36518118

Authors

Mohamed Rifaath

Preetha Santhakumar

J Selvaraj

Affiliations

Soon will be listed here.

Abstract

Colon cancer is the third most frequent cancer in humans. leaves are vegetable foods consumed by most people around the world; it has potential as an anticancer. Therefore it is of interest to investigate the effect of on beta catenin and Wnt mRNA expression in human colon cancer (HT-29) cells in vitro. Human Colon cancer cell line (HT-29) was purchased from the National Centre for Cell Sciences, Pune, India. Cell viability test was done by MTT assay. Gene expression analysis was done by Real Time-PCR. The obtained data were analyzed statistically by one-way analysis of variance and Duncan's multiple range test with Graph Pad Prism version 5 to analyze the significance of individual variations among the control and experimental groups. The significance was considered at p<0.05 level in Duncan's test. caused a marked increase in cell death in a dose dependent manner. At the end of 48 hours, maximum inhibition was at 300 and 400 µg/ml. has significantly reduced the mRNA expression of Wnt and beta catenin (p<0.05). Data showed that leaf extract has anticancer activity on Colon cancer cell lines (HT-29).

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References

Vivekanandhan K, Shanmugam P, Barabadi H, Arumugam V, Daniel Raj Daniel Paul Raj D, Sivasubramanian M . Emerging Therapeutic Approaches to Combat COVID-19: Present Status and Future Perspectives. Front Mol Biosci. 2021; 8:604447. PMC: 7983051. DOI: 10.3389/fmolb.2021.604447. View

Chakravarty S, Nandi S, Bhandari S, Das S . A Study on the Patterns of Psychiatric Referrals in a Tertiary Care Hospital in the North-Eastern Part of India. J Evol Med Dent Sci. 2020; 9(31):2217-2222. PMC: 7426194. View

J P, Marimuthu T, C K, Devadoss P, Kumar S . Prevalence and measurement of anterior loop of the mandibular canal using CBCT: A cross sectional study. Clin Implant Dent Relat Res. 2018; 20(4):531-534. DOI: 10.1111/cid.12609. View

Wahab P, Madhulaxmi M, Senthilnathan P, Muthusekhar M, Vohra Y, Abhinav R . Scalpel Versus Diathermy in Wound Healing After Mucosal Incisions: A Split-Mouth Study. J Oral Maxillofac Surg. 2018; 76(6):1160-1164. DOI: 10.1016/j.joms.2017.12.020. View

R H, Ramani P, Ramanathan A, R J, S G, Ramasubramanian A . CYP2 C9 polymorphism among patients with oral squamous cell carcinoma and its role in altering the metabolism of benzo[a]pyrene. Oral Surg Oral Med Oral Pathol Oral Radiol. 2020; 130(3):306-312. DOI: 10.1016/j.oooo.2020.06.021. View

Solai Prakash A, Devaraj E . Cytotoxic potentials of S. cumini methanolic seed kernel extract in human hepatoma HepG2 cells. Environ Toxicol. 2019; 34(12):1313-1319. DOI: 10.1002/tox.22832. View

Rajakumari R, Volova T, Oluwafemi O, Kumar S, Thomas S, Kalarikkal N . Grape seed extract-soluplus dispersion and its antioxidant activity. Drug Dev Ind Pharm. 2020; 46(8):1219-1229. DOI: 10.1080/03639045.2020.1788059. View

Ezhilarasan D . Critical role of estrogen in the progression of chronic liver diseases. Hepatobiliary Pancreat Dis Int. 2020; 19(5):429-434. DOI: 10.1016/j.hbpd.2020.03.011. View

Otsuki N, Dang N, Kumagai E, Kondo A, Iwata S, Morimoto C . Aqueous extract of Carica papaya leaves exhibits anti-tumor activity and immunomodulatory effects. J Ethnopharmacol. 2009; 127(3):760-7. DOI: 10.1016/j.jep.2009.11.024. View

10.

Nguyen T, Shaw P, Parat M, Hewavitharana A . Anticancer activity of Carica papaya: a review. Mol Nutr Food Res. 2012; 57(1):153-64. DOI: 10.1002/mnfr.201200388. View

11.

Sridharan G, Ramani P, Patankar S, Vijayaraghavan R . Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma. J Oral Pathol Med. 2019; 48(4):299-306. DOI: 10.1111/jop.12835. View

12.

Gajos-Michniewicz A, Czyz M . Modulation of WNT/β-catenin pathway in melanoma by biologically active components derived from plants. Fitoterapia. 2016; 109:283-92. DOI: 10.1016/j.fitote.2016.02.002. View

13.

Wadhwa R, Paudel K, Chin L, Hon C, Madheswaran T, Gupta G . Anti-inflammatory and anticancer activities of Naringenin-loaded liquid crystalline nanoparticles in vitro. J Food Biochem. 2020; 45(1):e13572. DOI: 10.1111/jfbc.13572. View

14.

Mudigonda S, Murugan S, Velavan K, Thulasiraman S, Raja V . Non-suturing microvascular anastomosis in maxillofacial reconstruction- a comparative study. J Craniomaxillofac Surg. 2020; 48(6):599-606. DOI: 10.1016/j.jcms.2020.04.005. View

15.

Shabgah A, Ezzatifar F, Aravindhan S, Zekiy A, Ahmadi M, Gheibihayat S . Shedding more light on the role of Midkine in hepatocellular carcinoma: New perspectives on diagnosis and therapy. IUBMB Life. 2021; 73(4):659-669. DOI: 10.1002/iub.2458. View

16.

Nambi G, Kamal W, Es S, Joshi S, Trivedi P . Spinal manipulation plus laser therapy versus laser therapy alone in the treatment of chronic non-specific low back pain: a randomized controlled study. Eur J Phys Rehabil Med. 2018; 54(6):880-889. DOI: 10.23736/S1973-9087.18.05005-0. View

17.

Morin P, Sparks A, Korinek V, Barker N, Clevers H, Vogelstein B . Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC. Science. 1997; 275(5307):1787-90. DOI: 10.1126/science.275.5307.1787. View