VASH1-SVBP and VASH2-SVBP Generate Different Detyrosination Profiles on Microtubules

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2022 Dec 13

PMID 36512346

Authors

Sacnicte Ramirez-Rios

Sung Ryul Choi

Chadni Sanyal

Thorsten B Blum

Christophe Bosc

Fatma Krichen

Eric Denarier

Jean-Marc Soleilhac

Beatrice Blot

Carsten Janke

Virginie Stoppin-Mellet

Maria M Magiera

Isabelle Arnal

Michel O Steinmetz

Marie-Jo Moutin

Affiliations

Soon will be listed here.

Abstract

The detyrosination/tyrosination cycle of α-tubulin is critical for proper cell functioning. VASH1-SVBP and VASH2-SVBP are ubiquitous enzymes involved in microtubule detyrosination, whose mode of action is little known. Here, we show in reconstituted systems and cells that VASH1-SVBP and VASH2-SVBP drive the global and local detyrosination of microtubules, respectively. We solved the cryo-electron microscopy structure of VASH2-SVBP bound to microtubules, revealing a different microtubule-binding configuration of its central catalytic region compared to VASH1-SVBP. We show that the divergent mode of detyrosination between the two enzymes is correlated with the microtubule-binding properties of their disordered N- and C-terminal regions. Specifically, the N-terminal region is responsible for a significantly longer residence time of VASH2-SVBP on microtubules compared to VASH1-SVBP. We suggest that this VASH region is critical for microtubule detachment and diffusion of VASH-SVBP enzymes on lattices. Our results suggest a mechanism by which VASH1-SVBP and VASH2-SVBP could generate distinct microtubule subpopulations and confined areas of detyrosinated lattices to drive various microtubule-based cellular functions.

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