Comparative Evaluation of Efficacy of Three Different Denture Cleansing Methods in Reducing Count in Removable Partial Denture Wearers: A Randomized Controlled Trial

Overview

Journal J Indian Prosthodont Soc

Specialty Dentistry

Date 2022 Dec 13

PMID 36511055

Authors

Arun Rajendran

Roshy George

Nicholas Mathew

M Ranjith

N Abu Nazar

Affiliations

Soon will be listed here.

Abstract

Aims: The study aims to find out the best possible method of cleaning the removable partial denture (RPD) by evaluating the Candida count limiting ability in RPD users using three different cleaning methods.

Settings And Design: The present study is randomized controlled trial. Three groups were formed with 20 participants in each. The groups were Group 1 RPD cleansing done using sterile saline and denture brush (negative control group), Group 2 RPD cleansing done using soap and denture brush and Group 3 RPD cleansing done using denture cleansing tablet and denture brush.

Materials And Methods: A baseline data and 15 days' postinsertion data of Candida count was recorded using swab collection, from the RPDs given. The swab was collected, cultured, and incubated using standard methods. Once Candida was identified using Sabouraud's dextrose agar, Candida albicans was further confirmed using germ tube test and cornmeal agar.

Statistical Analysis Used: The analysis was done using SPSS software (IBM Corp. Released 2010. IBM SPSS Statistics for Windows, Version 19.0. Armonk, NY: IBM Corp.). Paired t-test, was used to compare the number of colonies pre and postintervention. The difference between the groups was analyzed using one-way analysis of variance (ANOVA) and Tukey's post hoc analysis.

Results: The comparison of baseline data and postintervention data within each group using paired t test demonstrated statistically significant values; P = 0.046 and 0.000 in Group 2 and 3 respectively. The difference between the Candida colonies in three different groups after the intervention (15 Days) was analyzed using analysis of variance and found to be statistically significant with P = 0.004. Tukey's post hoc analysis was used to analyze the difference between the groups. It was concurred that there was a statistically significant difference between all three groups, but the difference in the mean was highest between the Group 1 and 3 (1210.99).

Conclusion: Within the limitations of the study, it is concluded that the use of denture cleansers and brush on a daily basis would actively reduce the number of C. albicans colony formation in RPD and thereby improve the overall health of denture wearer.

Citing Articles

Anti-biofilm effectiveness of protocols for cleaning complete dentures in hospitalized patients: a randomized controlled trial.

Gomes A, Maciel J, Garcia A, Coelho L, Rodrigues G, Porto V J Appl Oral Sci. 2024; 32:e20230381.

PMID: 38537031 PMC: 10984580. DOI: 10.1590/1678-7757-2022-0381.

References

Pereira-Cenci T, Fernandes F, Skupien J, Mesko M, Straioto F, Del Bel Cury A . Can new dentures decrease Candida levels?. Int J Prosthodont. 2013; 26(5):470-7. DOI: 10.11607/ijp.3047. View

Felton D, Cooper L, Duqum I, Minsley G, Guckes A, Haug S . Evidence-based guidelines for the care and maintenance of complete dentures: a publication of the American College of Prosthodontists. J Prosthodont. 2011; 20 Suppl 1:S1-S12. DOI: 10.1111/j.1532-849X.2010.00683.x. View

Prakash B, Shekar M, Maiti B, Karunasagar I, Padiyath S . Prevalence of Candida spp. among healthy denture and nondenture wearers with respect to hygiene and age. J Indian Prosthodont Soc. 2016; 15(1):29-32. PMC: 4762283. DOI: 10.4103/0972-4052.155041. View

Al-Saadi M . Effectiveness of Chemical and Microwave Disinfection on Denture Biofilm Fungi and the Influence of Disinfection on Denture Base Adaptation. J Indian Prosthodont Soc. 2015; 14(Suppl 1):24-30. PMC: 4502004. DOI: 10.1007/s13191-014-0354-2. View

Kumar P . The influence of , , and on strain in tissue conditioner at varying time intervals. J Indian Prosthodont Soc. 2020; 20(2):171-179. PMC: 7335031. DOI: 10.4103/jips.jips_366_19. View

Glass R, Conrad R, Bullard J, Goodson L, Mehta N, Lech S . Evaluation of microbial flora found in previously worn prostheses from the Northeast and Southwest regions of the United States. J Prosthet Dent. 2010; 103(6):384-9. DOI: 10.1016/S0022-3913(10)60083-2. View

Preshaw P, Walls A, Jakubovics N, Moynihan P, Jepson N, Loewy Z . Association of removable partial denture use with oral and systemic health. J Dent. 2011; 39(11):711-9. DOI: 10.1016/j.jdent.2011.08.018. View

Dills S, Olshan A, Goldner S, Brogdon C . Comparison of the antimicrobial capability of an abrasive paste and chemical-soak denture cleaners. J Prosthet Dent. 1988; 60(4):467-70. DOI: 10.1016/0022-3913(88)90250-8. View

Axe A, Varghese R, Bosma M, Kitson N, Bradshaw D . Dental health professional recommendation and consumer habits in denture cleansing. J Prosthet Dent. 2015; 115(2):183-8. DOI: 10.1016/j.prosdent.2015.08.007. View

10.

Perezous L, Stevenson G, Flaitz C, Goldschmidt M, Engelmeier R, Nichols C . The effect of complete dentures with a metal palate on candida species growth in HIV-infected patients. J Prosthodont. 2006; 15(5):306-15. DOI: 10.1111/j.1532-849X.2006.00127.x. View

11.

Fernandes F, Pereira-Cenci T, Silva W, Ricomini Filho A, Straioto F, Del Bel Cury A . Efficacy of denture cleansers on Candida spp. biofilm formed on polyamide and polymethyl methacrylate resins. J Prosthet Dent. 2011; 105(1):51-8. DOI: 10.1016/S0022-3913(10)60192-8. View

12.

Porwal A, Khandelwal M, Punia V, Sharma V . Effect of denture cleansers on color stability, surface roughness, and hardness of different denture base resins. J Indian Prosthodont Soc. 2017; 17(1):61-67. PMC: 5308072. DOI: 10.4103/0972-4052.197940. View

13.

Suresan V, Mantri S, Deogade S, Sumathi K, Panday P, Galav A . Denture hygiene knowledge, attitudes, and practices toward patient education in denture care among dental practitioners of Jabalpur city, Madhya Pradesh, India. J Indian Prosthodont Soc. 2016; 16(1):30-5. PMC: 4832801. DOI: 10.4103/0972-4052.175714. View

14.

Dikbas I, Koksal T, Calikkocaoglu S . Investigation of the cleanliness of dentures in a university hospital. Int J Prosthodont. 2006; 19(3):294-8. View

15.

Tripathi P, Singh Phukela S, Yadav B, Malhotra P . An study to evaluate and compare the surface roughness in heat-cured denture-based resin and injection-molded resin system as affected by two commercially available denture cleansers. J Indian Prosthodont Soc. 2018; 18(4):291-298. PMC: 6180744. DOI: 10.4103/jips.jips_335_17. View

16.

Lucena-Ferreira S, Gomes Cavalcanti I, Del Bel Cury A . Efficacy of denture cleansers in reducing microbial counts from removable partial dentures: a short-term clinical evaluation. Braz Dent J. 2013; 24(4):353-6. DOI: 10.1590/0103-6440201302183. View

17.

Kumar B, Sandhu P, Kumar A, Patil C . A comparative study for plaque removing efficacy between commonly used denture cleansers in India. J Indian Prosthodont Soc. 2017; 17(3):295-300. PMC: 5601496. DOI: 10.4103/jips.jips_2_17. View

18.

Jagger D, Harrison A, Rees J . The effectiveness of seven denture cleansers on tea stain removal from PMMA acrylic resin. Int J Prosthodont. 2002; 15(6):549-52. View

19.

Krishnamoorthy G, Narayana A, Peralam P, Balkrishanan D . To study the effect of oil when incorporated into tissue conditioner on its tensile strength and antifungal activity: An study. J Indian Prosthodont Soc. 2019; 19(3):225-232. PMC: 6685345. DOI: 10.4103/jips.jips_387_18. View

20.

Shah V, Shah D, Chauhan C, Doshi P, Kumar A . Evaluation of flexural strength and color stability of different denture base materials including flexible material after using different denture cleansers. J Indian Prosthodont Soc. 2016; 15(4):367-73. PMC: 4762351. DOI: 10.4103/0972-4052.164908. View