Paeoniflorin, a Constituent of Kami-shoyo-san, Suppresses Blood Glucose Levels in Postmenopausal Diabetic Mice by Promoting the Secretion of Estradiol from Adipocytes

Overview

Journal Biochem Biophys Rep

Specialty Biochemistry

Date 2022 Dec 13

PMID 36510583

Authors

Kyoko Kobayashi

Yu Ting Tang

Kenroh Sasaki

Affiliations

Soon will be listed here.

Abstract

Ovarian functional deterioration in women with climacteric disorders increases the prevalence of type 2 diabetes (T2D). Therefore, we revealed that paeoniflorin (PF), an ingredient of paeony root (PR), which is a constituent of Kami-shoyo-san (KS), promotes glucose uptake by increasing estradiol secretion from adipocytes. Adipocytes differentiated from 3T3-L1 cells were incubated in culture medium containing the extracts of KS, PR, KS excluding PR (KS-PR), or PF for 5 d at 37 °C and 5% CO. The estradiol and glucose concentrations in the medium were determined using enzyme-linked immunosorbent assay (ELISA). Next, PF (1 or 10 mg/kg) was subcutaneously injected into ovariectomized mice (12-week-old, ICR strain) once daily for 19 d to perform the glucose tolerance test and determine blood estradiol and adiponectin levels. The release of estradiol from 3T3-L1 adipocytes was significantly increased by KS, PR, KS-PR, and PF, and the increased estradiol level caused by KS was significantly decreased by excluding PF from KS (KS-PR). Glucose concentration in the medium was significantly decreased by KS and PF. In experiments, the 10 mg/kg PF-treated group showed significantly suppressed blood glucose levels at 0 and 30 min after d-glucose loading by intraperitoneal injection. These findings indicate that KS, which includes PR-containing PF as the main ingredient, may have the potential to prevent T2D caused by ovarian dysfunction in menopausal women by increasing estradiol secretion from adipocytes.

Citing Articles

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Kobayashi K, Tanabe A, Sasaki K Biomed Rep. 2024; 21(5):164.

PMID: 39268403 PMC: 11391171. DOI: 10.3892/br.2024.1852.

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