» Articles » PMID: 36503409

Accumulation of DNA Damage Alters MicroRNA Gene Transcription in Arabidopsis Thaliana

Overview
Journal BMC Plant Biol
Publisher Biomed Central
Specialty Biology
Date 2022 Dec 12
PMID 36503409
Authors
Affiliations
Soon will be listed here.
Abstract

Background: MicroRNAs (miRNAs) and other epigenetic modifications play fundamental roles in all eukaryotic biological processes. DNA damage repair is a key process for maintaining the genomic integrity of different organisms exposed to diverse stresses. However, the reaction of miRNAs in the DNA damage repair process is unclear.

Results: In this study, we found that the simultaneous mutation of zinc finger DNA 3'-phosphoesterase (ZDP) and AP endonuclease 2 (APE2), two genes that play overlapping roles in active DNA demethylation and base excision repair (BER), led to genome-wide alteration of miRNAs. The transcripts of newly transcribed miRNA-encoding genes (MIRs) decreased significantly in zdp/ape2, indicating that the mutation of ZDP and APE2 affected the accumulation of miRNAs at the transcriptional level. In addition, the introduction of base damage with the DNA-alkylating reagent methyl methanesulfonate (MMS) accelerated the reduction of miRNAs in zdp/ape2. Further mutation of FORMAMIDOPYRIMIDINE DNA GLYCOSYLASE (FPG), a bifunctional DNA glycosylase/lyase, rescued the accumulation of miRNAs in zdp/ape2, suggesting that the accumulation of DNA damage repair intermediates induced the transcriptional repression of miRNAs.

Conclusions: Our investigation indicates that the accumulation of DNA damage repair intermediates inhibit miRNAs accumulation by inhibiting MIR transcriptions.

Citing Articles

PTGS is dispensable for the initiation of epigenetic silencing of an active transposon in Arabidopsis.

Trasser M, Bohl-Viallefond G, Barragan-Borrero V, Diezma-Navas L, Loncsek L, Nordborg M EMBO Rep. 2024; 25(12):5780-5809.

PMID: 39511423 PMC: 11624286. DOI: 10.1038/s44319-024-00304-5.


Genome-wide analysis of Triticum aestivum bromodomain gene family and expression analysis under salt stress.

Wang Y, Shen S, Wu Z, Tao W, Zhang W, Yu P Planta. 2024; 260(5):117.

PMID: 39404907 DOI: 10.1007/s00425-024-04549-1.

References
1.
Baumberger N, Baulcombe D . Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs. Proc Natl Acad Sci U S A. 2005; 102(33):11928-33. PMC: 1182554. DOI: 10.1073/pnas.0505461102. View

2.
Mallory A, Reinhart B, Jones-Rhoades M, Tang G, Zamore P, Barton M . MicroRNA control of PHABULOSA in leaf development: importance of pairing to the microRNA 5' region. EMBO J. 2004; 23(16):3356-64. PMC: 514513. DOI: 10.1038/sj.emboj.7600340. View

3.
Pichiorri F, Suh S, Rocci A, De Luca L, Taccioli C, Santhanam R . RETRACTED: Downregulation of p53-inducible microRNAs 192, 194, and 215 impairs the p53/MDM2 autoregulatory loop in multiple myeloma development. Cancer Cell. 2010; 18(4):367-81. PMC: 3561766. DOI: 10.1016/j.ccr.2010.09.005. View

4.
Demple B, Harrison L . Repair of oxidative damage to DNA: enzymology and biology. Annu Rev Biochem. 1994; 63:915-48. DOI: 10.1146/annurev.bi.63.070194.004411. View

5.
Mallory A, Vaucheret H . Functions of microRNAs and related small RNAs in plants. Nat Genet. 2006; 38 Suppl:S31-6. DOI: 10.1038/ng1791. View