Anti-Melanogenesis Effect of Polysaccharide from on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells

Overview

Journal Nutrients

Date 2022 Dec 11

PMID 36501075

Authors

Mayila Kamilijiang

Deng Zang

Nuermaimaiti Abudukelimu

Nurbolat Aidarhan

Geyu Liu

Haji Akber Aisa

Affiliations

Soon will be listed here.

Abstract

As one of the prominent medicinal plants listed in the Chinese pharmacopoeia (2020), (Kar. et Kir.) Sch.-Bip was demonstrated to possess various therapeutic effects. In our recent research, we extracted the polysaccharides from (SIP) at optimal conditions and conducted further structure elucidation on the main fraction as well as the confirmation of its possible anti-inflammatory activity. Hence, in this work, we assessed the in vitro antioxidant activity and anti-melanogenesis effects of the crude SIP in forskolin-induced B16F10 melanoma cells. The results show that SIP possessed strong antioxidant activity and was effective in concentration-dependently decreasing melanin formation and inhibiting tyrosinase activity in forskolin-induced B16F10 cells. Based on these results, the inhibitory mechanism of melanogenesis was investigated by measuring Tyrosinase (TYR), Tyrosinase related protein-1 (TRP-1), Tyrosinase related protein-2 (TRP-2), Microphthalmia-associated transcription factor (MITF), cAMP-response element binding protein (CREB), mitogen-activated protein kinases (MAPK) signaling protein members, and β-catenin degradation in forskolin-induced B16F10 cells. The anti-melanogenesis response of SIP might be attributed to the regulation of c-Jun -terminal kinase (JNK) phosphorylation and β-catenin degradation pathways. These results suggest that polysaccharides from possess a strong anti-melanogenic effect, and thus could be used as a high-value natural material for skin whitening in cosmeceutical industries.

Citing Articles

Noradrenergic Projections from the Locus Coeruleus to the Medial Prefrontal Cortex Enhances Stress Coping Behavior in Mice Following Long-Term Intermittent Fasting.

Zhao Z, Chen J, Zhan H, Fang C, Hua L, Deng H Neuromolecular Med. 2024; 26(1):47.

PMID: 39580532 DOI: 10.1007/s12017-024-08818-w.

Antimelanogenic and Antioxidant Effects of Postbioics of Strains in α-MSH-Induced B16F10 Melanoma Cells via CREB/MITF and MAPKs Signaling Pathway.

Lee H, Lee Y, Park K, Lee N, Paik H J Microbiol Biotechnol. 2024; 34(11):2279-2289.

PMID: 39468979 PMC: 11637832. DOI: 10.4014/jmb.2408.08015.

Current and potential future biological uses of (Falc.) Lipsch: A comprehensive review.

Elnour A, Abdurahman N Heliyon. 2024; 10(18):e37790.

PMID: 39323795 PMC: 11422592. DOI: 10.1016/j.heliyon.2024.e37790.

Immunomodulatory Activity of Polysaccharides Isolated from L. and Ledeb.

Schepetkin I, Danilets M, Ligacheva A, Trofimova E, Selivanova N, Sherstoboev E Molecules. 2023; 28(18).

PMID: 37764432 PMC: 10536955. DOI: 10.3390/molecules28186655.

Evaluation of anti-oxidant and antimelanogenic effects of the essential oil and extracts of × in B16F10 murine melanoma cell line.

Hadipour E, Rezazadeh Kafash M, Emami S, Asili J, Boghrati Z, Tayarani-Najaran Z Iran J Basic Med Sci. 2023; 26(9):1076-1082.

PMID: 37605721 PMC: 10440142. DOI: 10.22038/IJBMS.2023.69734.15182.

References

Bu J, Ma P, Chen Z, Zhou W, Fu Y, Li L . Inhibition of MITF and tyrosinase by paeonol-stimulated JNK/SAPK to reduction of phosphorylated CREB. Am J Chin Med. 2008; 36(2):245-63. DOI: 10.1142/S0192415X08005758. View

Costin G, Hearing V . Human skin pigmentation: melanocytes modulate skin color in response to stress. FASEB J. 2007; 21(4):976-94. DOI: 10.1096/fj.06-6649rev. View

Sung J, Park S, Seo D, Lee J, Hong S, Hong S . Antioxidative and skin-whitening effect of an aqueous extract of Salicornia herbacea. Biosci Biotechnol Biochem. 2009; 73(3):552-6. DOI: 10.1271/bbb.80601. View

Latres E, Chiaur D, Pagano M . The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin. Oncogene. 1999; 18(4):849-54. DOI: 10.1038/sj.onc.1202653. View

Miyamura Y, Coelho S, Wolber R, Miller S, Wakamatsu K, Zmudzka B . Regulation of human skin pigmentation and responses to ultraviolet radiation. Pigment Cell Res. 2007; 20(1):2-13. DOI: 10.1111/j.1600-0749.2006.00358.x. View

Bourhim T, Villareal M, Gadhi C, Hafidi A, Isoda H . Depigmenting effect of argan press-cake extract through the down-regulation of Mitf and melanogenic enzymes expression in B16 murine melanoma cells. Cytotechnology. 2018; 70(5):1389-1397. PMC: 6214846. DOI: 10.1007/s10616-018-0232-6. View

Xiao W, Li X, Li N, Bolati M, Wang X, Jia X . Sesquiterpene lactones from Saussurea involucrata. Fitoterapia. 2011; 82(7):983-7. DOI: 10.1016/j.fitote.2011.05.015. View

Swalwell H, Latimer J, Haywood R, Birch-Machin M . Investigating the role of melanin in UVA/UVB- and hydrogen peroxide-induced cellular and mitochondrial ROS production and mitochondrial DNA damage in human melanoma cells. Free Radic Biol Med. 2011; 52(3):626-634. DOI: 10.1016/j.freeradbiomed.2011.11.019. View

Balcos M, Kim S, Jeong H, Yun H, Baek K, Kwon N . Docosahexaenoic acid inhibits melanin synthesis in murine melanoma cells in vitro through increasing tyrosinase degradation. Acta Pharmacol Sin. 2014; 35(4):489-95. PMC: 4813719. DOI: 10.1038/aps.2013.174. View

10.

LERNER A, Fitzpatrick T . Biochemistry of melanin formation. Physiol Rev. 1950; 30(1):91-126. DOI: 10.1152/physrev.1950.30.1.91. View

11.

Yao L, Zhao Q, Xiao J, Sun J, Yuan X, Zhao B . Composition and antioxidant activity of the polysaccharides from cultivated Saussurea involucrata. Int J Biol Macromol. 2011; 50(3):849-53. DOI: 10.1016/j.ijbiomac.2011.11.012. View

12.

Slominski A, Tobin D, Shibahara S, Wortsman J . Melanin pigmentation in mammalian skin and its hormonal regulation. Physiol Rev. 2004; 84(4):1155-228. DOI: 10.1152/physrev.00044.2003. View

13.

Chik W, Zhu L, Fan L, Yi T, Zhu G, Gou X . Saussurea involucrata: A review of the botany, phytochemistry and ethnopharmacology of a rare traditional herbal medicine. J Ethnopharmacol. 2015; 172:44-60. DOI: 10.1016/j.jep.2015.06.033. View

14.

Cho M, Ryu M, Jeong Y, Chung Y, Kim D, Cho H . Cardamonin suppresses melanogenesis by inhibition of Wnt/beta-catenin signaling. Biochem Biophys Res Commun. 2009; 390(3):500-5. DOI: 10.1016/j.bbrc.2009.09.124. View

15.

Azam M, Kim J, Choi C, Choi J, Lee B, Kim H . Sargahydroquinoic Acid Suppresses Hyperpigmentation by cAMP and ERK1/2-Mediated Downregulation of MITF in α-MSH-Stimulated B16F10 Cells. Foods. 2021; 10(10). PMC: 8534327. DOI: 10.3390/foods10102254. View

16.

Wang T, Wang R, Chen H, Shang M, Cai S . Alkyl and phenolic glycosides from Saussurea stella. Fitoterapia. 2013; 88:38-43. DOI: 10.1016/j.fitote.2013.03.027. View

17.

Wu L, Lin Y, Yang S, Weng Y, Tsai Y . Antimelanogenic effect of c-phycocyanin through modulation of tyrosinase expression by upregulation of ERK and downregulation of p38 MAPK signaling pathways. J Biomed Sci. 2011; 18:74. PMC: 3210093. DOI: 10.1186/1423-0127-18-74. View

18.

Lajis A, Ariff A . Discovery of new depigmenting compounds and their efficacy to treat hyperpigmentation: Evidence from in vitro study. J Cosmet Dermatol. 2019; 18(3):703-727. DOI: 10.1111/jocd.12900. View

19.

Hseu Y, Chen X, Gowrisankar Y, Yen H, Chuang J, Yang H . The Skin-Whitening Effects of Ectoine via the Suppression of α-MSH-Stimulated Melanogenesis and the Activation of Antioxidant Nrf2 Pathways in UVA-Irradiated Keratinocytes. Antioxidants (Basel). 2020; 9(1). PMC: 7022695. DOI: 10.3390/antiox9010063. View

20.

Serre C, Busuttil V, Botto J . Intrinsic and extrinsic regulation of human skin melanogenesis and pigmentation. Int J Cosmet Sci. 2018; 40(4):328-347. DOI: 10.1111/ics.12466. View