An Efficient Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry Method for the Analysis of Methyl Farnesoate Released in Growth Medium by

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Dec 11

PMID 36500684

Authors

Nicolo Riboni

Antonio Suppa

Annamaria Buschini

Federica Bianchi

Valeria Rossi

Gessica Gorbi

Maria Careri

Affiliations

Soon will be listed here.

Abstract

Methyl farnesoate (MF), a juvenile hormone, can influence phenotypic traits and stimulates male production in daphnids. MF is produced endogenously in response to stressful conditions, but it is not known whether this hormone can also be released into the environment to mediate stress signaling. In the present study, for the first time, a reliable solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) method was developed and validated for the ultra-trace analysis of MF released in growth medium by maintained in presence of crowding w/o MK801, a putative upstream inhibitor of MF endogenous production. Two different clonal lineages, I and S clones, which differ in the sensitivity to the stimuli leading to male production, were also compared. A detection limit of 1.3 ng/L was achieved, along with good precision and trueness, thus enabling the quantitation of MF at ultra-trace level. The achieved results demonstrated the release of MF by both clones at the 20 ng/L level in control conditions, whereas a significant decrease in the presence of crowding was assessed. As expected, a further reduction was obtained in the presence of MK801. These findings strengthen the link between environmental stimuli and the MF signaling pathway. , by releasing the juvenile hormone MF in the medium, could regulate population dynamics by means of an autoregulatory feedback loop that controls the intra- and extra-individual-level release of MF produced by endogenous biosynthesis.

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References

Huang S, Chen G, Ye N, Kou X, Zhu F, Shen J . Solid-phase microextraction: An appealing alternative for the determination of endogenous substances - A review. Anal Chim Acta. 2019; 1077:67-86. DOI: 10.1016/j.aca.2019.05.054. View

Borst D, Tsukimura B . Quantification of methyl farnesoate levels in hemolymph by high-performance liquid chromatography. J Chromatogr. 1991; 545(1):71-8. DOI: 10.1016/s0021-9673(01)88696-9. View

Booksmythe I, Gerber N, Ebert D, Kokko H . Daphnia females adjust sex allocation in response to current sex ratio and density. Ecol Lett. 2018; 21(5):629-637. DOI: 10.1111/ele.12929. View

Xie X, Zhu D, Li Y, Qiu X, Cui X, Tang J . Hemolymph Levels of Methyl Farnesoate During Ovarian Development of the Swimming Crab Portunus trituberculatus, and Its Relation to Transcript Levels of HMG-CoA Reductase and Farnesoic Acid O-Methyltransferase. Biol Bull. 2015; 228(2):118-24. DOI: 10.1086/BBLv228n2p118. View

Xu J, Hu Q, Liu X, Wei S, Zheng J, Lin W . Determination of the mass transfer coefficients in direct immersion solid-phase microextraction. J Sep Sci. 2020; 43(9-10):1847-1853. DOI: 10.1002/jssc.201901345. View

Imai M, Naraki Y, Tochinai S, Miura T . Elaborate regulations of the predator-induced polyphenism in the water flea Daphnia pulex: kairomone-sensitive periods and life-history tradeoffs. J Exp Zool A Ecol Genet Physiol. 2009; 311(10):788-95. DOI: 10.1002/jez.565. View

Ramirez C, Nouzova M, Michalkova V, Fernandez-Lima F, Noriega F . Common structural features facilitate the simultaneous identification and quantification of the five most common juvenile hormones by liquid chromatography-tandem mass spectrometry. Insect Biochem Mol Biol. 2019; 116:103287. PMC: 6983331. DOI: 10.1016/j.ibmb.2019.103287. View

Toyota K, Miyakawa H, Yamaguchi K, Shigenobu S, Ogino Y, Tatarazako N . NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex. BMC Genomics. 2015; 16:186. PMC: 4372037. DOI: 10.1186/s12864-015-1392-9. View

Jones D, Jones G, Teal P, Hammac C, Messmer L, Osborne K . Suppressed production of methyl farnesoid hormones yields developmental defects and lethality in Drosophila larvae. Gen Comp Endocrinol. 2009; 165(2):244-54. PMC: 3277837. DOI: 10.1016/j.ygcen.2009.07.006. View

10.

Kai Z, Yin Y, Zhang Z, Huang J, Tobe S, Chen S . A rapid quantitative assay for juvenile hormones and intermediates in the biosynthetic pathway using gas chromatography tandem mass spectrometry. J Chromatogr A. 2018; 1538:67-74. DOI: 10.1016/j.chroma.2018.01.030. View

11.

Groothuis F, Heringa M, Nicol B, Hermens J, Blaauboer B, Kramer N . Dose metric considerations in in vitro assays to improve quantitative in vitro-in vivo dose extrapolations. Toxicology. 2013; 332:30-40. DOI: 10.1016/j.tox.2013.08.012. View

12.

Smith P, Clare A, Rees H, Prescott M, Wainwright G, Thorndyke M . Identification of methyl farnesoate in the cypris larva of the barnacle, Balanus amphitrite, and its role as a juvenile hormone. Insect Biochem Mol Biol. 2000; 30(8-9):885-90. DOI: 10.1016/s0965-1748(00)00062-x. View

13.

Toyota K, Miyakawa H, Hiruta C, Furuta K, Ogino Y, Shinoda T . Methyl farnesoate synthesis is necessary for the environmental sex determination in the water flea Daphnia pulex. J Insect Physiol. 2015; 80:22-30. DOI: 10.1016/j.jinsphys.2015.02.002. View

14.

Miyakawa H, Toyota K, Hirakawa I, Ogino Y, Miyagawa S, Oda S . A mutation in the receptor Methoprene-tolerant alters juvenile hormone response in insects and crustaceans. Nat Commun. 2013; 4:1856. DOI: 10.1038/ncomms2868. View

15.

Augusto F, Carasek E, Gomes Costa Silva R, Rivellino S, Batista A, Martendal E . New sorbents for extraction and microextraction techniques. J Chromatogr A. 2009; 1217(16):2533-42. DOI: 10.1016/j.chroma.2009.12.033. View

16.

Bianchi F, Careri M, Mangia A, Mattarozzi M, Musci M . Experimental design for the optimization of the extraction conditions of polycyclic aromatic hydrocarbons in milk with a novel diethoxydiphenylsilane solid-phase microextraction fiber. J Chromatogr A. 2008; 1196-1197:41-5. DOI: 10.1016/j.chroma.2008.04.018. View

17.

Lengyel F, Westerlund S, Kaib M . Juvenile hormone III influences task-specific cuticular hydrocarbon profile changes in the ant Myrmicaria eumenoides. J Chem Ecol. 2006; 33(1):167-81. DOI: 10.1007/s10886-006-9185-x. View

18.

Montes R, Rodil R, Neuparth T, Santos M, Cela R, Quintana J . A simple and sensitive approach to quantify methyl farnesoate in whole arthropods by matrix-solid phase dispersion and gas chromatography-mass spectrometry. J Chromatogr A. 2017; 1508:158-162. DOI: 10.1016/j.chroma.2017.06.001. View

19.

Olmstead A, LeBlanc G . Juvenoid hormone methyl farnesoate is a sex determinant in the crustacean Daphnia magna. J Exp Zool. 2002; 293(7):736-9. DOI: 10.1002/jez.10162. View

20.

Teal P, Jones D, Jones G, Torto B, Nyasembe V, Borgemeister C . Identification of methyl farnesoate from the hemolymph of insects. J Nat Prod. 2014; 77(2):402-5. DOI: 10.1021/np400807v. View