Antimicrobial and Antibiofilm Effect of Brazilian Green Propolis Aqueous Extract Against Dental Anaerobic Bacteria

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Dec 11

PMID 36500223

Authors

Maria Angelica de Sa Assis

Lucas de Paula Ramos

Amjad Abu Hasna

Talita Suelen de Queiroz

Thais Cristine Pereira

Patricia Michelle Nagai de Lima

Andresa Aparecida Berretta

Maria Cristina Marcucci

Claudio Antonio Talge Carvalho

Luciane Dias de Oliveira

Affiliations

Soon will be listed here.

Abstract

Green propolis may represent a promising therapeutic alternative against dental anaerobic pathogens because of its antimicrobial action. This study aimed to evaluate the antimicrobial and antibiofilm actions of Brazilian green propolis aqueous extract (BGP-AqExt) against dental anaerobic bacteria. The minimum inhibitory concentration (MIC) and minimum microbicide concentration (MMC) of the extract were determined against the standard strains (ATCC) of , , and . BGP-AqExt was chemically characterized by high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis. Antibiofilm action was measured by MTT and crystal violet tests. The data were statistically analyzed by ANOVA and Tukey (5%) tests. The extract had antimicrobial action against all tested anaerobic bacteria, with an MIC value of 55 mg/mL for all bacteria, an MMC of 27.5 mg/mL for and and 55 mg/mL for . Chemically, BGP-AqExt is composed of quercetin, gallic acid, caffeic and p-coumaric acid, drupani, kaempferol and Artepillin C. Significant reductions in biomass and metabolic action of biofilms were found after BGP-AqExt application. Therefore, BGP-AqExt has an antimicrobial and antibiofilm effect against dental anaerobic bacteria.

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