Extracellular Vesicles in Regenerative Processes Associated with Muscle Injury Recovery of Professional Athletes Undergoing Sub Maximal Strength Rehabilitation

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Dec 11

PMID 36499243

Authors

Giulia Catitti

Maria Concetta Cufaro

Domenico De Bellis

Ilaria Cicalini

Simone Vespa

Federico Tonelli

Giulia Miscia

Lorenzo Secondi

Pasquale Simeone

Vincenzo De Laurenzi

Damiana Pieragostino

Piero Del Boccio

Paola Lanuti

Affiliations

Soon will be listed here.

Abstract

Platelet-rich plasma (PRP) has great potential in regenerative medicine. In addition to the well-known regenerative potential of secreted growth factors, extracellular vesicles (EVs) are emerging as potential key players in the regulation of tissue repair. However, little is known about their therapeutic potential as regenerative agents. In this study, we have identified and subtyped circulating EVs (platelet-, endothelial-, and leukocyte-derived EVs) in the peripheral blood of athletes recovering from recent muscular injuries and undergoing a submaximal strength rehabilitation program. We found a significant increase in circulating platelet-derived EVs at the end of the rehabilitation program. Moreover, EVs from PRP samples were isolated by fluorescence-activated cell sorting and analyzed by label-free proteomics. The proteomic analysis of PRP-EVs revealed that 32% of the identified proteins were associated to "defense and immunity", and altogether these proteins were involved in vesicle-mediated transport (GO: 0016192; FDR = 3.132 × 10), as well as in wound healing (GO: 0042060; FDR = 4.252 × 10) and in the events regulating such a process (GO: 0061041; FDR = 2.812 × 10). Altogether, these data suggest that platelet-derived EVs may significantly contribute to the regeneration potential of PRP preparations.

Citing Articles

Rehabilomics Strategies Enabled by Cloud-Based Rehabilitation: Scoping Review.

Oh S, Lee S J Med Internet Res. 2025; 27:e54790.

PMID: 39874565 PMC: 11815311. DOI: 10.2196/54790.

Beyond Blood Clotting: The Many Roles of Platelet-Derived Extracellular Vesicles.

Muttiah B, Ng S, Lokanathan Y, Ng M, Law J Biomedicines. 2024; 12(8).

PMID: 39200314 PMC: 11351396. DOI: 10.3390/biomedicines12081850.

Advances in Platelet Rich Plasma-Derived Extracellular Vesicles for Regenerative Medicine: A Systematic-Narrative Review.

Anitua E, Troya M, Falcon-Perez J, Lopez-Sarrio S, Gonzalez E, Alkhraisat M Int J Mol Sci. 2023; 24(17).

PMID: 37685849 PMC: 10488108. DOI: 10.3390/ijms241713043.

CAR-T-Derived Extracellular Vesicles: A Promising Development of CAR-T Anti-Tumor Therapy.

Pagotto S, Simeone P, Brocco D, Catitti G, De Bellis D, Vespa S Cancers (Basel). 2023; 15(4).

PMID: 36831396 PMC: 9954490. DOI: 10.3390/cancers15041052.

References

Chandler W . Microparticle counts in platelet-rich and platelet-free plasma, effect of centrifugation and sample-processing protocols. Blood Coagul Fibrinolysis. 2012; 24(2):125-32. DOI: 10.1097/MBC.0b013e32835a0824. View

Patel A, Selzman C, Kumpati G, McKellar S, Bull D . Evaluation of autologous platelet rich plasma for cardiac surgery: outcome analysis of 2000 patients. J Cardiothorac Surg. 2016; 11(1):62. PMC: 4828785. DOI: 10.1186/s13019-016-0452-9. View

Rigamonti E, Zordan P, Sciorati C, Rovere-Querini P, Brunelli S . Macrophage plasticity in skeletal muscle repair. Biomed Res Int. 2014; 2014:560629. PMC: 4016840. DOI: 10.1155/2014/560629. View

Brocco D, Lanuti P, Simeone P, Bologna G, Pieragostino D, Cufaro M . Circulating Cancer Stem Cell-Derived Extracellular Vesicles as a Novel Biomarker for Clinical Outcome Evaluation. J Oncol. 2019; 2019:5879616. PMC: 6885781. DOI: 10.1155/2019/5879616. View

Cufaro M, Pieragostino D, Lanuti P, Rossi C, Cicalini I, Federici L . Extracellular Vesicles and Their Potential Use in Monitoring Cancer Progression and Therapy: The Contribution of Proteomics. J Oncol. 2019; 2019:1639854. PMC: 6590542. DOI: 10.1155/2019/1639854. View

Cela I, Cufaro M, Fucito M, Pieragostino D, Lanuti P, Sallese M . Proteomic Investigation of the Role of Nucleostemin in Nucleophosmin-Mutated OCI-AML 3 Cell Line. Int J Mol Sci. 2022; 23(14). PMC: 9317519. DOI: 10.3390/ijms23147655. View

Melki I, Tessandier N, Zufferey A, Boilard E . Platelet microvesicles in health and disease. Platelets. 2017; 28(3):214-221. DOI: 10.1080/09537104.2016.1265924. View

Thery C, Witwer K, Aikawa E, Alcaraz M, Anderson J, Andriantsitohaina R . Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2019; 7(1):1535750. PMC: 6322352. DOI: 10.1080/20013078.2018.1535750. View

Buca D, DAntonio F, Buca D, Di Sebastiano F, Simeone P, Di Girolamo R . Extracellular Vesicles in pregnancy: Their potential role as a liquid biopsy. J Reprod Immunol. 2022; 154:103734. DOI: 10.1016/j.jri.2022.103734. View

10.

Lanuti P, Ciccocioppo F, Bonanni L, Marchisio M, Lachmann R, Tabet N . Amyloid-specific T-cells differentiate Alzheimer's disease from Lewy body dementia. Neurobiol Aging. 2012; 33(11):2599-611. DOI: 10.1016/j.neurobiolaging.2012.01.004. View

11.

Rossi C, Cicalini I, Cufaro M, Agnifili L, Mastropasqua L, Lanuti P . Multi-Omics Approach for Studying Tears in Treatment-Naïve Glaucoma Patients. Int J Mol Sci. 2019; 20(16). PMC: 6721157. DOI: 10.3390/ijms20164029. View

12.

Tao S, Yuan T, Rui B, Zhu Z, Guo S, Zhang C . Exosomes derived from human platelet-rich plasma prevent apoptosis induced by glucocorticoid-associated endoplasmic reticulum stress in rat osteonecrosis of the femoral head via the Akt/Bad/Bcl-2 signal pathway. Theranostics. 2017; 7(3):733-750. PMC: 5327646. DOI: 10.7150/thno.17450. View

13.

Casolo A, Farina D, Falla D, Bazzucchi I, Felici F, Del Vecchio A . Strength Training Increases Conduction Velocity of High-Threshold Motor Units. Med Sci Sports Exerc. 2019; 52(4):955-967. DOI: 10.1249/MSS.0000000000002196. View

14.

Dohan Ehrenfest D, Andia I, Zumstein M, Zhang C, Pinto N, Bielecki T . Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. Muscles Ligaments Tendons J. 2014; 4(1):3-9. PMC: 4049647. View

15.

Guo S, Tao S, Yin W, Qi X, Yuan T, Zhang C . Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model. Theranostics. 2017; 7(1):81-96. PMC: 5196887. DOI: 10.7150/thno.16803. View

16.

Ziemkiewicz N, Hilliard G, Pullen N, Garg K . The Role of Innate and Adaptive Immune Cells in Skeletal Muscle Regeneration. Int J Mol Sci. 2021; 22(6). PMC: 8005179. DOI: 10.3390/ijms22063265. View

17.

Coumans F, Brisson A, Buzas E, Dignat-George F, Drees E, El-Andaloussi S . Methodological Guidelines to Study Extracellular Vesicles. Circ Res. 2017; 120(10):1632-1648. DOI: 10.1161/CIRCRESAHA.117.309417. View

18.

Wang H, Wang B . Extracellular vesicle microRNAs mediate skeletal muscle myogenesis and disease. Biomed Rep. 2016; 5(3):296-300. PMC: 4997983. DOI: 10.3892/br.2016.725. View

19.

Antich-Rossello M, Forteza-Genestra M, Monjo M, Ramis J . Platelet-Derived Extracellular Vesicles for Regenerative Medicine. Int J Mol Sci. 2021; 22(16). PMC: 8395287. DOI: 10.3390/ijms22168580. View

20.

Muchedzi T, Roberts S . A systematic review of the effects of platelet rich plasma on outcomes for patients with knee osteoarthritis and following total knee arthroplasty. Surgeon. 2017; 16(4):250-258. DOI: 10.1016/j.surge.2017.08.004. View