Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances in and Suggests a Link Between Various Stresses and Antibiotic Production

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Dec 11

PMID 36499130

Authors

Lejeune Clara

Cornu David

Sago Laila

Redeker Virginie

Virolle Marie-Joelle

Affiliations

Soon will be listed here.

Abstract

and constitute model strains to study the regulation of antibiotics biosynthesis in species since these closely related strains possess the same pathways directing the biosynthesis of various antibiotics but only produces them. To get a better understanding of the origin of the contrasted abilities of these strains to produce bioactive specialized metabolites, these strains were grown in conditions of phosphate limitation or proficiency and a comparative analysis of their transcriptional/regulatory proteins was carried out. The abundance of the vast majority of the 355 proteins detected greatly differed between these two strains and responded differently to phosphate availability. This study confirmed, consistently with previous studies, that suffers from nitrogen stress. This stress likely triggers the degradation of the nitrogen-rich peptidoglycan cell wall in order to recycle nitrogen present in its constituents, resulting in cell wall stress. When an altered cell wall is unable to fulfill its osmo-protective function, the bacteria also suffer from osmotic stress. This study thus revealed that these three stresses are intimately linked in . The aggravation of these stresses leading to an increase of antibiotic biosynthesis, the connection between these stresses, and antibiotic production are discussed.

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