Bioactivity-boosting Strategy Based on Combination of Anti-allergic O-methylated Catechin with a Citrus Flavanone, Hesperetin

Overview

Journal J Nat Med

Publisher Springer

Date 2022 Dec 9

PMID 36494586

Authors

Yoshinori Fujimura

Takanori Yoshimoto

Konatsu Fujino

Ayaka Nezu

Yuki Marugame

Jaehoon Bae

Motofumi Kumazoe

Hirofumi Tachibana

Affiliations

Soon will be listed here.

Abstract

Many patients with allergies have anxiety about taking anti-allergic medicines due to their side effects and increased medical expenses. Thus, developing functional foods/agricultural products for allergy prevention is strongly desired. In this study, we revealed that a Citrus flavanone, hesperetin, amplified IgE/antigen-mediated degranulation-inhibitory potency of anti-allergic catechin, (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3''Me), in the rat basophilic/mast cell line RBL-2H3. Hesperetin also significantly elevated the activation of acid sphingomyelinase (ASM), essential for eliciting anti-allergic effect of EGCG3''Me through the cell surficial protein, 67-kDa laminin receptor (67LR). Furthermore, oral administration of the highly absorbent hesperidin, α-glucosyl hesperidin, also enhanced the inhibitory potency of EGCG3''Me-rich 'Benifuuki' green tea (Camellia sinensis L.) on passive cutaneous anaphylaxis (PCA) reaction evoked by IgE/antigen in BALB/c mice. These observations indicate that hesperetin amplifies the ability of EGCG3''Me to inhibit the IgE/antigen-mediated degranulation through activating ASM signaling.

Citing Articles

Fermented plant product (FPP) suppresses immediate hypersensitivity reactions with impaired high-affinity IgE receptor (FcεRI) signaling.

Kodama T, Yokoyama A, Nishioka Y, Kawasaki R, Teshima A, Maeda A Cytotechnology. 2025; 77(2):69.

PMID: 40012927 PMC: 11861467. DOI: 10.1007/s10616-025-00729-3.

Effects of Dietary Components on Mast Cells: Possible Use as Nutraceuticals for Allergies?.

Kaag S, Lorentz A Cells. 2023; 12(22).

PMID: 37998337 PMC: 10670325. DOI: 10.3390/cells12222602.

References

Hayat K, Iqbal H, Malik U, Bilal U, Mushtaq S . Tea and its consumption: benefits and risks. Crit Rev Food Sci Nutr. 2014; 55(7):939-54. DOI: 10.1080/10408398.2012.678949. View

Pei Z, Shi G, Kondo S, Ito M, Maekawa A, Suzuki M . Adenovirus vectors lacking virus-associated RNA expression enhance shRNA activity to suppress hepatitis C virus replication. Sci Rep. 2013; 3:3575. PMC: 3868971. DOI: 10.1038/srep03575. View

Gilfillan A, Tkaczyk C . Integrated signalling pathways for mast-cell activation. Nat Rev Immunol. 2006; 6(3):218-30. DOI: 10.1038/nri1782. View

Maeda-Yamamoto M, Inagaki N, Kitaura J, Chikumoto T, Kawahara H, Kawakami Y . O-methylated catechins from tea leaves inhibit multiple protein kinases in mast cells. J Immunol. 2004; 172(7):4486-92. DOI: 10.4049/jimmunol.172.7.4486. View

Maeda-Yamamoto M, Ema K, Monobe M, Shibuichi I, Shinoda Y, Yamamoto T . The efficacy of early treatment of seasonal allergic rhinitis with benifuuki green tea containing O-methylated catechin before pollen exposure: an open randomized study. Allergol Int. 2009; 58(3):437-44. DOI: 10.2332/allergolint.08-OA-0066. View

Masuda S, Maeda-Yamamoto M, Usui S, Fujisawa T . 'Benifuuki' green tea containing o-methylated catechin reduces symptoms of Japanese cedar pollinosis: a randomized, double-blind, placebo-controlled trial. Allergol Int. 2014; 63(2):211-7. DOI: 10.2332/allergolint.13-OA-0620. View

Tachibana H, Koga K, Fujimura Y, Yamada K . A receptor for green tea polyphenol EGCG. Nat Struct Mol Biol. 2004; 11(4):380-1. DOI: 10.1038/nsmb743. View

Hong Byun E, Fujimura Y, Yamada K, Tachibana H . TLR4 signaling inhibitory pathway induced by green tea polyphenol epigallocatechin-3-gallate through 67-kDa laminin receptor. J Immunol. 2010; 185(1):33-45. DOI: 10.4049/jimmunol.0903742. View

Umeda D, Yano S, Yamada K, Tachibana H . Green tea polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor. J Biol Chem. 2007; 283(6):3050-3058. DOI: 10.1074/jbc.M707892200. View

10.

Kumazoe M, Sugihara K, Tsukamoto S, Huang Y, Tsurudome Y, Suzuki T . 67-kDa laminin receptor increases cGMP to induce cancer-selective apoptosis. J Clin Invest. 2013; 123(2):787-99. PMC: 3561824. DOI: 10.1172/JCI64768. View

11.

Tsukamoto S, Hirotsu K, Kumazoe M, Goto Y, Sugihara K, Suda T . Green tea polyphenol EGCG induces lipid-raft clustering and apoptotic cell death by activating protein kinase Cδ and acid sphingomyelinase through a 67 kDa laminin receptor in multiple myeloma cells. Biochem J. 2012; 443(2):525-34. DOI: 10.1042/BJ20111837. View

12.

Fujimura Y, Umeda D, Yano S, Maeda-Yamamoto M, Yamada K, Tachibana H . The 67kDa laminin receptor as a primary determinant of anti-allergic effects of O-methylated EGCG. Biochem Biophys Res Commun. 2007; 364(1):79-85. DOI: 10.1016/j.bbrc.2007.09.095. View

13.

Fujimura Y, Fujino K, Yoshimoto T, Nezu A, Marugame Y, Bae J . Eriodictyol-Amplified 67-kDa Laminin Receptor Signaling Potentiates the Antiallergic Effect of -Methylated Catechin. J Nat Prod. 2021; 84(6):1823-1830. DOI: 10.1021/acs.jnatprod.1c00337. View

14.

Barreca D, Gattuso G, Bellocco E, Calderaro A, Trombetta D, Smeriglio A . Flavanones: Citrus phytochemical with health-promoting properties. Biofactors. 2017; 43(4):495-506. DOI: 10.1002/biof.1363. View

15.

Kumazoe M, Fujimura Y, Hidaka S, Kim Y, Murayama K, Takai M . Metabolic profiling-based data-mining for an effective chemical combination to induce apoptosis of cancer cells. Sci Rep. 2015; 5:9474. PMC: 4379465. DOI: 10.1038/srep09474. View

16.

Fujimura Y, Kumazoe M, Tachibana H . 67-kDa Laminin Receptor-Mediated Cellular Sensing System of Green Tea Polyphenol EGCG and Functional Food Pairing. Molecules. 2022; 27(16). PMC: 9416087. DOI: 10.3390/molecules27165130. View

17.

Yamada M, Tanabe F, Arai N, Mitsuzumi H, Miwa Y, Kubota M . Bioavailability of glucosyl hesperidin in rats. Biosci Biotechnol Biochem. 2006; 70(6):1386-94. DOI: 10.1271/bbb.50657. View

18.

Murata M, Shimizu Y, Marugame Y, Nezu A, Fujino K, Yamada S . EGCG down-regulates MuRF1 expression through 67-kDa laminin receptor and the receptor signaling is amplified by eriodictyol. J Nat Med. 2020; 74(4):673-679. DOI: 10.1007/s11418-020-01417-6. View

19.

Bae J, Kumazoe M, Fujimura Y, Tachibana H . Diallyl disulfide potentiates anti-obesity effect of green tea in high-fat/high-sucrose diet-induced obesity. J Nutr Biochem. 2018; 64:152-161. DOI: 10.1016/j.jnutbio.2018.10.014. View

20.

Huang E, Strate L, Ho W, Lee S, Chan A . A prospective study of aspirin use and the risk of gastrointestinal bleeding in men. PLoS One. 2011; 5(12):e15721. PMC: 3012090. DOI: 10.1371/journal.pone.0015721. View