Evaluation of <em>Echinococcus</em> DNA by Polymerase Chain Reaction (PCR) in Cystic <em>Echinococcosis</em> of the Liver

Overview

Journal Turk J Surg

Specialty General Surgery

Date 2022 Dec 9

PMID 36483165

Authors

Mehmet Tolga Kiris

Sefa Ergun

Ozan Akinci

Sevgi Ergin

Mehmet Velidedeoglu

Bekir Sami Kocazeybek

Ertugrul Goksoy

Affiliations

Soon will be listed here.

Abstract

Objectives: The aim of this study was to determine the DNA and genotypes of in liver cyst hydatids isolated in humans.

Material And Methods: This study was conducted prospectively at the Department of General Surgery of the Cerrahpaşa School of Medicine, University of İstanbul-Cerrahpaşa, between January 2015 and June 2016 in 30 patients who were operated on for cystic DNA was analyzed using the Polymerase Chain Reaction (PCR) method in the cyst samples (protoscolex and/or germinative membrane) obtained during the operation, and genotype was determined in the PCR positive samples by sequence analysis. At the same time, indirect hemagglutination (IHA) was used to test for the presence of antibodies in the patients' blood.

Results: DNA was found in 29 out of 30 cystic of the liver samples. All of the 29 cystic samples were found to be G1 (sheep) species. Also, IHA was positive in 22 patients and negative in eight patients.

Conclusion: In the present study, G1 species was the most commonly seen liver cystic species. We suggest that a vaccine, which could be developed against prevalent regional genotypes, would be efficacious in the prevention of the disease with a cause of mortality and morbidity.

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