The Role of Reduced Glutathione on the Activity of Adenosine Deaminase, Antioxidative System, and Aluminum and Zinc Levels in Experimental Aluminum Toxicity

Overview

Journal Biol Trace Elem Res

Date 2022 Dec 1

PMID 36456741

Authors

Onur Atakisi

Kezban Yildiz Dalginli

Canan Gulmez

Destan Kalacay

Emine Atakisi

Taasilkan Toktamamatovna Zhumabaeva

Tunay Kontas Askar

Ruken Esra Demirdogen

Affiliations

Soon will be listed here.

Abstract

Aluminum (Al) is one of the most abundant element in the world. But aluminum exposure and accumulation causes serious diseases, related with free radicals. Reduced glutathione (GSH) is a tripeptide with intracellular antioxidant effects. This study aimed to investigate the role of GSH on adenosine deaminase (ADA), antioxidant system, and aluminum and zinc (Zn) levels in acute aluminum toxicity. In this study, Sprague-Dawley rats (n = 32) were used. The rats were divided into four equal groups (n = 8). Group I received 0.5 mL intraperitoneal injection of 0.9% saline solution (NaCI), Group II received single-dose AlCI, Group III was given GSH for seven days, and Group IV was given AlCI single dose, and at the same time, 100 mg/kg GSH was given for seven days. At the end of the trial, blood samples were collected by cardiac puncture. Serum total antioxidant status (TAS) and Zn levels were lower in the aluminum-administered group than the control group. In contrast, plasma total oxidant status (TOS) and aluminum concentrations and ADA activity were found higher in the aluminum-administered group than in the control group. Unlike the other groups, group GSH administrated with aluminum was similar to the control group. As a result, GSH administration has a regulatory effect on ADA activity, antioxidant system, and Zn levels in experimental aluminum toxicity. In addition, GSH may reduce the oxidant capacity increased by Al administration and may have a tolerant role on the accumulated serum Al levels. But long-term experimental Al toxicity studies are needed to reach a firm conclusion.

Citing Articles

Investigation of the Effect of Omega-3 Fatty Acids on Antioxidant System and Serum Aluminum, Zinc, and Iron Levels in Acute Aluminum Toxicity.

Atakisi E, Atakisi O, Ozturkler M, Dalginli K, Ozbey C Biol Trace Elem Res. 2024; .

PMID: 39365382 DOI: 10.1007/s12011-024-04402-2.

References

Jaishankar M, Tseten T, Anbalagan N, Mathew B, Beeregowda K . Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol. 2015; 7(2):60-72. PMC: 4427717. DOI: 10.2478/intox-2014-0009. View

Deloncle R, Huguet F, Babin P, Fernandez B, Quellard N, Guillard O . Chronic administration of aluminium L-glutamate in young mature rats: effects on iron levels and lipid peroxidation in selected brain areas. Toxicol Lett. 1999; 104(1-2):65-73. DOI: 10.1016/s0378-4274(98)00345-2. View

Capriello T, Di Meglio G, De Maio A, Scudiero R, Bianchi A, Trifuoggi M . Aluminium exposure leads to neurodegeneration and alters the expression of marker genes involved to parkinsonism in zebrafish brain. Chemosphere. 2022; 307(Pt 1):135752. DOI: 10.1016/j.chemosphere.2022.135752. View

Ruiperez F, Mujika J, Ugalde J, Exley C, Lopez X . Pro-oxidant activity of aluminum: promoting the Fenton reaction by reducing Fe(III) to Fe(II). J Inorg Biochem. 2012; 117:118-23. DOI: 10.1016/j.jinorgbio.2012.09.008. View

Paz L, Moura L, Feio D, Cardoso M, Ximenes W, Montenegro R . Evaluation of in vivo and in vitro toxicological and genotoxic potential of aluminum chloride. Chemosphere. 2017; 175:130-137. DOI: 10.1016/j.chemosphere.2017.02.011. View

Bounous G, Molson J . The antioxidant system. Anticancer Res. 2003; 23(2B):1411-5. View

Irshad M, Chaudhuri P . Oxidant-antioxidant system: role and significance in human body. Indian J Exp Biol. 2003; 40(11):1233-9. View

Jones D . Radical-free biology of oxidative stress. Am J Physiol Cell Physiol. 2008; 295(4):C849-68. PMC: 2575825. DOI: 10.1152/ajpcell.00283.2008. View

Allen J, Bradley R . Effects of oral glutathione supplementation on systemic oxidative stress biomarkers in human volunteers. J Altern Complement Med. 2011; 17(9):827-33. PMC: 3162377. DOI: 10.1089/acm.2010.0716. View

10.

Kaplowitz N . The importance and regulation of hepatic glutathione. Yale J Biol Med. 1981; 54(6):497-502. PMC: 2596047. View

11.

DeLeve L, Kaplowitz N . Glutathione metabolism and its role in hepatotoxicity. Pharmacol Ther. 1991; 52(3):287-305. DOI: 10.1016/0163-7258(91)90029-l. View

12.

Richie Jr J, Nichenametla S, Neidig W, Calcagnotto A, Haley J, Schell T . Randomized controlled trial of oral glutathione supplementation on body stores of glutathione. Eur J Nutr. 2014; 54(2):251-63. DOI: 10.1007/s00394-014-0706-z. View

13.

Gao Z, Wang X, Zhang H, Lin F, Liu C, Dong K . The roles of adenosine deaminase in autoimmune diseases. Autoimmun Rev. 2020; 20(1):102709. DOI: 10.1016/j.autrev.2020.102709. View

14.

Gakis C . Adenosine deaminase (ADA) isoenzymes ADA1 and ADA2: diagnostic and biological role. Eur Respir J. 1996; 9(4):632-3. DOI: 10.1183/09031936.96.09040632. View

15.

Flinn A, Gennery A . Adenosine deaminase deficiency: a review. Orphanet J Rare Dis. 2018; 13(1):65. PMC: 5916829. DOI: 10.1186/s13023-018-0807-5. View

16.

Whitmore K, Gaspar H . Adenosine Deaminase Deficiency - More Than Just an Immunodeficiency. Front Immunol. 2016; 7:314. PMC: 4985714. DOI: 10.3389/fimmu.2016.00314. View

17.

Kumar V, Sharma A . Adenosine: an endogenous modulator of innate immune system with therapeutic potential. Eur J Pharmacol. 2009; 616(1-3):7-15. DOI: 10.1016/j.ejphar.2009.05.005. View

18.

Bagheri S, Saboury A, Haertle T . Adenosine deaminase inhibition. Int J Biol Macromol. 2019; 141:1246-1257. DOI: 10.1016/j.ijbiomac.2019.09.078. View

19.

Hasko G, Cronstein B . Adenosine: an endogenous regulator of innate immunity. Trends Immunol. 2003; 25(1):33-9. DOI: 10.1016/j.it.2003.11.003. View

20.

Camici M, Garcia-Gil M, Tozzi M . The Inside Story of Adenosine. Int J Mol Sci. 2018; 19(3). PMC: 5877645. DOI: 10.3390/ijms19030784. View