Phyllosphere Microbial Community of Cigar Tobacco and Its Corresponding Metabolites

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Nov 28

PMID 36439836

Authors

Tiantian Liu

Shiping Guo

Chongde Wu

Ruina Zhang

Qiu Zhong

Hongzhi Shi

Rongqing Zhou

Yanqing Qin

Yao Jin

Affiliations

Soon will be listed here.

Abstract

Cigar is made of a typical fermented tobacco where the microbiota inhabits within an alkaline environment. Our current understanding on cigar fermentation is far from thorough. This work employed both high-throughput sequencing and chromatography-mass spectrometric technologies to provide new scientific reference for this specific fermented system. Typical cigar samples from different regions (the Caribbeans, South America, East Asia, and Southeast Asia) were investigated. The results show that , , , and were the predominant phyla in the cigar samples. Rather than the fungal community, it was the bacterial community structures that played vital roles to differentiate the cigar from different regions: was the dominant genus in the Americas; was the dominant genus in Southeast Asia; while in East Asia, there was no dominant genus. Such differences in community structure then affected the microflora metabolism. The correlation between microbiota and metabolites revealed that , , and were significantly correlated with sclareolide; were positively associated with isophorone. was significantly and positively correlated with L-nicotine and hexadecanoic acid, methyl ester. GRAPHICAL ABSTRACT.

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