Key Infection Stages Defending Heat Stress in High-temperature-resistant F. Sp. Isolates

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Nov 28

PMID 36439801

Authors

Meihui Zhang

Aolin Wang

Cheng Zhang

Fei Xu

Wei Liu

Jieru Fan

Zhanhong Ma

Yilin Zhou

Affiliations

Soon will be listed here.

Abstract

With the increase of temperature in the winter wheat-growing regions in China, the high-temperature-resistant f. sp. () isolates developed in the fields. To clarify the key infection stages and the roles of heat shock protein (HSP) genes of high-temperature-resistant isolates defending high temperature, 3 high-temperature-resistant and 3 sensitive isolates were selected from 55 isolates after determination of temperature sensitivity. And then they were used to investigate the infection stages and the expression levels of genes, including , , and , at 18°C and 25°C. The formation frequency of abnormal appressoria and inhibition rate of haustoria formation of high-temperature-resistant isolates at 25°C were lower than those of high-temperature-sensitive isolates, while major axis of microcolonies of high-temperature-resistant isolates was higher than those of high-temperature-sensitive isolates at 25°C. The results indicated that haustoria formation and hyphal expansion were the key infection stages of defense against heat stress in high-temperature-resistant isolates. Further analyses of genes found the expression levels of and were upregulated at 24 and 72 h post-inoculation in high-temperature-resistant isolates, while no significant difference was observed for and genes. Taken together, the basis of high-temperature-resistant isolates is associated with induced expression of and response to heat stress in haustoria formation and hyphal expansion stages.

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