Effects of Daehwang-Hwanglyoun-Sasim-Tang on Brain Injury and Cognitive Function in Mice Caused by Bilateral Common Carotid Artery Stenosis

Overview

Journal Int J Med Sci

Specialty General Medicine

Date 2022 Nov 28

PMID 36438916

Authors

Myeong-Hwa Lee

Chiyeon Lim

Sehyun Lim

Suin Cho

Kyung-Min Kim

Affiliations

Soon will be listed here.

Abstract

Among geriatric diseases, cerebrovascular disease ranks fourth according to the Causes of Death Statistics in 2019, Korea, and is the most common cause of acquired disorders in adults. Daehwang-Hwanglyoun-Sasim-Tang (DHST), a herbal prescription consisting of two herbal medicines, Rhei Rhizoma and Coptidis Rhizoma, has been reported to have anti-inflammatory, antioxidant, and anticancer effects. This study was conducted to confirm the anti-inflammatory mechanism of DHST treatment in ischemic brain injury and to confirm the role of DHST in cognitive function improvement. C57BL/6 male mice were randomly divided into four groups (sham operation, bilateral common carotid artery stenosis (BCAS) control, experimental group administered 5 mL/kg DHST, experimental group administered 50 mL/kg DHST), with each group containing five mice. After 1 week, DHST was orally administered for 4 weeks, 5 days a week, and then behavioral evaluation of learning and memory was performed. In addition, morphological changes in the neurons in the CA1 region of the hippocampus were observed. Inflammation-related factors were evaluated using western blot analysis. In the 50 mL/kg DHST (H-DHST) group, the expression of apoptosis-related proteins was reduced and neuronal damage was suppressed in the hippocampal CA1 region. However, cognitive improvement was observed in the H-DHST group that was attributable to anti-inflammatory and antiapoptotic pathways. In the 5 mL/kg DHST group, no significant effect was observed compared with the control group.

References

Sanae F, Komatsu Y, Chisaki K, Kido T, Ishige A, Hayashi H . Effects of San'o-shashin-to and the constituent herbal medicines on theophylline-induced increase in arterial blood pressure of rats. Biol Pharm Bull. 2001; 24(10):1137-41. DOI: 10.1248/bpb.24.1137. View

Kraeuter A, Guest P, Sarnyai Z . The Y-Maze for Assessment of Spatial Working and Reference Memory in Mice. Methods Mol Biol. 2018; 1916:105-111. DOI: 10.1007/978-1-4939-8994-2_10. View

Zhao Y, Gong C . From chronic cerebral hypoperfusion to Alzheimer-like brain pathology and neurodegeneration. Cell Mol Neurobiol. 2014; 35(1):101-10. PMC: 11486181. DOI: 10.1007/s10571-014-0127-9. View

Zhou R, Guo F, Xiang C, Zhang Y, Yang H, Zhang J . Systematic Study of Crucial Transcription Factors of Alkaloids against Cerebral Ischemia-Reperfusion Injury. ACS Chem Neurosci. 2021; 12(13):2308-2319. DOI: 10.1021/acschemneuro.0c00730. View

Jorm A, Jolley D . The incidence of dementia: a meta-analysis. Neurology. 1998; 51(3):728-33. DOI: 10.1212/wnl.51.3.728. View

Lewis T, Shapiro P, Ahn N . Signal transduction through MAP kinase cascades. Adv Cancer Res. 1998; 74:49-139. DOI: 10.1016/s0065-230x(08)60765-4. View

Friedemann T, Otto B, Klatschke K, Schumacher U, Tao Y, Leung A . Coptis chinensis Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells. J Ethnopharmacol. 2014; 155(1):607-15. DOI: 10.1016/j.jep.2014.06.004. View

Wu J, Nakashima S, Shigyo M, Yamasaki M, Ikuno S, Morikawa A . Antihypertensive constituents in Sanoshashinto. J Nat Med. 2020; 74(2):421-433. PMC: 7929964. DOI: 10.1007/s11418-019-01382-9. View

Singh S, Srivastav S, Castellani R, Plascencia-Villa G, Perry G . Neuroprotective and Antioxidant Effect of Ginkgo biloba Extract Against AD and Other Neurological Disorders. Neurotherapeutics. 2019; 16(3):666-674. PMC: 6694352. DOI: 10.1007/s13311-019-00767-8. View

10.

Tahamtan M, Allahtavakoli M, Abbasnejad M, Roohbakhsh A, Taghipour Z, Taghavi M . Exercise preconditioning improves behavioral functions following transient cerebral ischemia induced by 4-vessel occlusion (4-VO) in rats. Arch Iran Med. 2013; 16(12):697-704. DOI: 0131612/AIM.004. View

11.

Kitagawa K, Matsumoto M, Yang G, Mabuchi T, Yagita Y, Hori M . Cerebral ischemia after bilateral carotid artery occlusion and intraluminal suture occlusion in mice: evaluation of the patency of the posterior communicating artery. J Cereb Blood Flow Metab. 1998; 18(5):570-9. DOI: 10.1097/00004647-199805000-00012. View

12.

Lai T, Zhang S, Wang Y . Excitotoxicity and stroke: identifying novel targets for neuroprotection. Prog Neurobiol. 2013; 115:157-88. DOI: 10.1016/j.pneurobio.2013.11.006. View

13.

Widmann C, Gibson S, Jarpe M, Johnson G . Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human. Physiol Rev. 1999; 79(1):143-80. DOI: 10.1152/physrev.1999.79.1.143. View

14.

Jung W, Kwon S, Cho S, Park S, Moon S, Park J . The Effects of Chunghyul-Dan (A Korean Medicine Herbal Complex) on Cardiovascular and Cerebrovascular Diseases: A Narrative Review. Evid Based Complement Alternat Med. 2016; 2016:2601740. PMC: 4909900. DOI: 10.1155/2016/2601740. View

15.

Stebbins G, Nyenhuis D, Wang C, Cox J, Freels S, Bangen K . Gray matter atrophy in patients with ischemic stroke with cognitive impairment. Stroke. 2008; 39(3):785-93. DOI: 10.1161/STROKEAHA.107.507392. View

16.

Lee B, Choi Y, Kim H, Kim S, Hahm D, Lee H . Protective effects of methanol extract of Acori graminei rhizoma and Uncariae Ramulus et Uncus on ischemia-induced neuronal death and cognitive impairments in the rat. Life Sci. 2003; 74(4):435-50. DOI: 10.1016/j.lfs.2003.06.034. View

17.

Sarti C, Pantoni L, Bartolini L, Inzitari D . Cognitive impairment and chronic cerebral hypoperfusion: what can be learned from experimental models. J Neurol Sci. 2002; 203-204:263-6. DOI: 10.1016/s0022-510x(02)00302-7. View

18.

Wang M, Hayashi H, Horinokita I, Asada M, Iwatani Y, Liu J . Neuroprotective effects of Senkyunolide I against glutamate-induced cells death by attenuating JNK/caspase-3 activation and apoptosis. Biomed Pharmacother. 2021; 140:111696. DOI: 10.1016/j.biopha.2021.111696. View

19.

Kalaria R, Maestre G, Arizaga R, Friedland R, Galasko D, Hall K . Alzheimer's disease and vascular dementia in developing countries: prevalence, management, and risk factors. Lancet Neurol. 2008; 7(9):812-26. PMC: 2860610. DOI: 10.1016/S1474-4422(08)70169-8. View

20.

Candas D, Li J . MnSOD in oxidative stress response-potential regulation via mitochondrial protein influx. Antioxid Redox Signal. 2013; 20(10):1599-617. PMC: 3942709. DOI: 10.1089/ars.2013.5305. View