A Banana Transcriptional Repressor MaAP2a Participates in Fruit Starch Degradation During Postharvest Ripening

Overview

Journal Front Plant Sci

Date 2022 Nov 28

PMID 36438126

Authors

Yunyi Xiao

Ying Li

Lejun Ouyang

Aiguo Yin

Bo Xu

Ling Zhang

Jianye Chen

Jinfeng Liu

Affiliations

Soon will be listed here.

Abstract

Fruit postharvest ripening is a crucial course for many fruits with significant conversion of biosubstance, which forms an intricate regulatory network. Ethylene facilitates the ripening process in banana with a remarkable change of fruit starch, but the mechanism adjusting the expression of starch degradation-related enzyme genes is incompletely discovered. Here, we describe a banana APETALA2 transcription factor (MaAP2a) identified as a transcriptional repressor with its powerful transcriptional inhibitory activity. The transcriptional level of MaAP2a gradually decreased with the transition of banana fruit ripening, suggesting a passive role of MaAP2a in banana fruit ripening. Moreover, MaAP2a is a classic nucleoprotein and encompasses transcriptional repressor domain (EAR, LxLxLx). More specifically, protein-DNA interaction assays found that MaAP2a repressed the expression of 15 starch degradation-related genes comprising , , , , , /, /, and binding to the GCC-box or AT-rich motif of their promoters. Overall, these results reveal an original MaAP2a-mediated negative regulatory network involved in banana postharvest starch breakdown, which advances our cognition on banana fruit ripening and offers additional reference values for banana varietal improvement.

Citing Articles

Molecular Characteristics and Functional Identification of a Key Alpha-Amylase-Encoding Gene in .

Sun P, Zhu Z, Jin Z, Xie J, Miao H, Liu J Int J Mol Sci. 2024; 25(14).

PMID: 39063074 PMC: 11276985. DOI: 10.3390/ijms25147832.

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