Structure and Properties of Bioactive Glass-Modified Calcium Phosphate/Calcium Sulfate Biphasic Porous Self-Curing Bone Repair Materials and Preliminary Research on Their Osteogenic Effect

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2022 Nov 26

PMID 36431384

Authors

Tao Tan

Danyang Song

Suning Hu

Xiangrui Li

Mei Li

Lei Wang

Hailan Feng

Affiliations

Soon will be listed here.

Abstract

In this study, calcium phosphate (CP)/calcium sulfate biphasic bone repair materials were modified with bioactive-glass (BG) to construct a self-curing bone repair material. Tetracalcium phosphate, calcium hydrogen phosphate dihydrate, and calcium sulfate hemihydrate (CSH) with different BG ratios and phosphate solution were reacted to prepare a porous self-curing bone repair material (CP/CSH/BG). The solidification time was about 12 min, and the material was morphologically stable in 24 h. The porosity was about 50%, with a pore size around 200 μm. The strength of CP/CSH/BG was approaching trabecular bone, and could be gradually degraded in Tris-HCl solution. MC3T3-E1 cells were cultured in the leaching solution of the materials. Cytotoxicity was detected using Cell Counting Kit 8 assays, and the expression of osteogenesis-related biomarkers was detected using quantitative real-time reverse transcription PCR (qRT-PCR). The results showed that all BG groups had increased ALP and ARS staining, implying that the BG groups could promote osteoblast mineralization in vitro. qRT-PCR showed significant upregulation of bone-related gene expression (Osx, Ocn, Runx2, and Col1) in the 20% BG group (p < 0.05). Therefore, the CP/CSH/BG self-curing bone repair materials can promote osteogenesis, and might be applied for bone regeneration, especially for polymorphic bone defect repair.

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References

Lin C, Schek R, Mistry A, Shi X, Mikos A, Krebsbach P . Functional bone engineering using ex vivo gene therapy and topology-optimized, biodegradable polymer composite scaffolds. Tissue Eng. 2005; 11(9-10):1589-98. DOI: 10.1089/ten.2005.11.1589. View

Ren L, Zhang Z, Deng C, Zhang N, Li D . Antibacterial and pro-osteogenic effects of β-Defensin-2-loaded mesoporous bioglass. Dent Mater J. 2020; 40(2):464-471. DOI: 10.4012/dmj.2020-105. View

Pereira R, Menezes J, Bonardi J, Griza G, Okamoto R, Hochuli-Vieira E . Comparative study of volumetric changes and trabecular microarchitecture in human maxillary sinus bone augmentation with bioactive glass and autogenous bone graft: a prospective and randomized assessment. Int J Oral Maxillofac Surg. 2017; 47(5):665-671. DOI: 10.1016/j.ijom.2017.11.016. View

Liu H, Liu X, Zhang L, Ai H, Cui F . Improvement on the performance of bone regeneration of calcium sulfate hemihydrate by adding mineralized collagen. Tissue Eng Part A. 2010; 16(6):2075-84. DOI: 10.1089/ten.TEA.2009.0669. View

Liu Y, Lim J, Teoh S . Review: development of clinically relevant scaffolds for vascularised bone tissue engineering. Biotechnol Adv. 2012; 31(5):688-705. DOI: 10.1016/j.biotechadv.2012.10.003. View

Zhang N, Gao T, Wang Y, Wang Z, Zhang P, Liu J . Environmental pH-controlled loading and release of protein on mesoporous hydroxyapatite nanoparticles for bone tissue engineering. Mater Sci Eng C Mater Biol Appl. 2014; 46:158-65. DOI: 10.1016/j.msec.2014.10.014. View

Zheng A, Wang X, Xin X, Peng L, Su T, Cao L . Promoting lacunar bone regeneration with an injectable hydrogel adaptive to the microenvironment. Bioact Mater. 2022; 21:403-421. PMC: 9483602. DOI: 10.1016/j.bioactmat.2022.08.031. View

Mahdavi F, Salehi A, Seyedjafari E, Mohammadi-Sangcheshmeh A, Ardeshirylajimi A . Bioactive glass ceramic nanoparticles-coated poly(l-lactic acid) scaffold improved osteogenic differentiation of adipose stem cells in equine. Tissue Cell. 2017; 49(5):565-572. DOI: 10.1016/j.tice.2017.07.003. View

Fairbairn P, Leventis M, Mangham C, Horowitz R . Alveolar Ridge Preservation Using a Novel Synthetic Grafting Material: A Case with Two-Year Follow-Up. Case Rep Dent. 2018; 2018:6412806. PMC: 5816876. DOI: 10.1155/2018/6412806. View

10.

Tadic D, Beckmann F, Schwarz K, Epple M . A novel method to produce hydroxyapatite objects with interconnecting porosity that avoids sintering. Biomaterials. 2004; 25(16):3335-40. DOI: 10.1016/j.biomaterials.2003.10.007. View

11.

Kim H, Kar A, Kaja A, Lim E, Choi W, Son W . More weighted cancellous bone can be harvested from the proximal tibia with less donor site pain than anterior iliac crest corticocancellous bone harvesting: retrospective review. J Orthop Surg Res. 2021; 16(1):220. PMC: 7995739. DOI: 10.1186/s13018-021-02364-y. View

12.

Mao K, Yang Y, Li J, Hao L, Tang P, Wang Z . Investigation of the histology and interfacial bonding between carbonated hydroxyapatite cement and bone. Biomed Mater. 2009; 4(4):045003. DOI: 10.1088/1748-6041/4/4/045003. View

13.

Moore W, Graves S, Bain G . Synthetic bone graft substitutes. ANZ J Surg. 2001; 71(6):354-61. View

14.

Wu D, Zhou L, Lin J, Chen J, Huang W, Chen Y . Immediate implant placement in anterior teeth with grafting material of autogenous tooth bone vs xenogenic bone. BMC Oral Health. 2019; 19(1):266. PMC: 6889614. DOI: 10.1186/s12903-019-0970-7. View

15.

Arabnejad S, Johnston R, Pura J, Singh B, Tanzer M, Pasini D . High-strength porous biomaterials for bone replacement: A strategy to assess the interplay between cell morphology, mechanical properties, bone ingrowth and manufacturing constraints. Acta Biomater. 2015; 30:345-356. DOI: 10.1016/j.actbio.2015.10.048. View

16.

Jambhekar S, Kernen F, Bidra A . Clinical and histologic outcomes of socket grafting after flapless tooth extraction: a systematic review of randomized controlled clinical trials. J Prosthet Dent. 2015; 113(5):371-82. DOI: 10.1016/j.prosdent.2014.12.009. View

17.

McAllister B, Haghighat K . Bone augmentation techniques. J Periodontol. 2007; 78(3):377-96. DOI: 10.1902/jop.2007.060048. View

18.

Tee B, Desai K, Kennedy K, Sonnichsen B, Kim D, Fields H . Reconstructing jaw defects with MSCs and PLGA-encapsulated growth factors. Am J Transl Res. 2016; 8(6):2693-704. PMC: 4931163. View

19.

Huhtinen R, Sandeman S, Rose S, Fok E, Howell C, Froberg L . Examining porous bio-active glass as a potential osteo-odonto-keratoprosthetic skirt material. J Mater Sci Mater Med. 2013; 24(5):1217-27. DOI: 10.1007/s10856-013-4881-x. View

20.

Chen H, Shen M, Shen J, Li Y, Wang R, Ye M . A new injectable quick hardening anti-collapse bone cement allows for improving biodegradation and bone repair. Biomater Adv. 2022; 141:213098. DOI: 10.1016/j.bioadv.2022.213098. View