Oxygenated Water Increases Seizure Threshold in Various Rodent Seizure Models

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Nov 26

PMID 36430603

Authors

Hyeok Hee Kwon

Seung Yeon Jung

Hyewon Park

Hyo Jung Shin

Dong Woon Kim

Hee-Jung Song

Joon Won Kang

Affiliations

Soon will be listed here.

Abstract

Oxygenated water (OW) contains more oxygen than normal drinking water. It may induce oxygen enrichment in the blood and reduce oxidative stress. Hypoxia and oxidative stress could be involved in epilepsy. We aimed to examine the effects of OW-treated vs. control on four rodent models of epilepsy: (1) prenatal betamethasone priming with postnatal N-methyl-D-aspartate (NMDA)-triggered spasm, (2) no prenatal betamethasone, (3) repetitive kainate injection, and (4) intraperitoneal pilocarpine. We evaluated, in (1) and (2), the latency to onset and the total number of spasms; (3) the number of kainate injections required to induce epileptic seizures; (4) spontaneous recurrent seizures (SRS) (numbers and duration). In model (1), the OW-treated group showed significantly increased latency to onset and a decreased total number of spasms; in (2), OW completely inhibited spasms; in (3), the OW-treated group showed a significantly decreased number of injections required to induce epileptic seizures; and in (4), in the OW-treated group, the duration of a single SRS was significantly reduced. In summary, OW may increase the seizure threshold. Although the underlying mechanism remains unclear, OW may provide an adjunctive alternative for patients with refractory epilepsy.

References

Houser C . Granule cell dispersion in the dentate gyrus of humans with temporal lobe epilepsy. Brain Res. 1990; 535(2):195-204. DOI: 10.1016/0006-8993(90)91601-c. View

Huff J, Fountain N . Pathophysiology and definitions of seizures and status epilepticus. Emerg Med Clin North Am. 2010; 29(1):1-13. DOI: 10.1016/j.emc.2010.08.001. View

Reyes R, Brennan A, Shen Y, Baldwin Y, Swanson R . Activation of neuronal NMDA receptors induces superoxide-mediated oxidative stress in neighboring neurons and astrocytes. J Neurosci. 2012; 32(37):12973-8. PMC: 3478885. DOI: 10.1523/JNEUROSCI.1597-12.2012. View

Tse K, Puttachary S, Beamer E, Sills G, Thippeswamy T . Advantages of repeated low dose against single high dose of kainate in C57BL/6J mouse model of status epilepticus: behavioral and electroencephalographic studies. PLoS One. 2014; 9(5):e96622. PMC: 4011859. DOI: 10.1371/journal.pone.0096622. View

Shi X, Ju J, Zou L, Wang J, Shang N, Zhao J . Increased precipitation of spasms in an animal model of infantile spasms by prenatal stress exposure. Life Sci. 2016; 152:171-7. DOI: 10.1016/j.lfs.2016.03.047. View

Song L, Zheng J, Li H, Jia N, Suo Z, Cai Q . Prenatal stress causes oxidative damage to mitochondrial DNA in hippocampus of offspring rats. Neurochem Res. 2008; 34(4):739-45. DOI: 10.1007/s11064-008-9838-y. View

Ozugur S, Kunz L, Straka H . Relationship between oxygen consumption and neuronal activity in a defined neural circuit. BMC Biol. 2020; 18(1):76. PMC: 7333326. DOI: 10.1186/s12915-020-00811-6. View

Umpierre A, Bennett I, Nebeker L, Newell T, Tian B, Thomson K . Repeated low-dose kainate administration in C57BL/6J mice produces temporal lobe epilepsy pathology but infrequent spontaneous seizures. Exp Neurol. 2016; 279:116-126. PMC: 5382800. DOI: 10.1016/j.expneurol.2016.02.014. View

Wing S, Askew E, Luetkemeier M, Ryujin D, Kamimori G, Grissom C . Lack of effect of Rhodiola or oxygenated water supplementation on hypoxemia and oxidative stress. Wilderness Environ Med. 2003; 14(1):9-16. DOI: 10.1580/1080-6032(2003)014[0009:loeoro]2.0.co;2. View

10.

Baek H, Yi M, Pandit S, Park J, Kwon H, Zhang E . Altered expression of KCC2 in GABAergic interneuron contributes prenatal stress-induced epileptic spasms in infant rat. Neurochem Int. 2016; 97:57-64. DOI: 10.1016/j.neuint.2016.05.006. View

11.

Velisek L, Jehle K, Asche S, Veliskova J . Model of infantile spasms induced by N-methyl-D-aspartic acid in prenatally impaired brain. Ann Neurol. 2007; 61(2):109-19. DOI: 10.1002/ana.21082. View

12.

Kovac S, Domijan A, Walker M, Abramov A . Seizure activity results in calcium- and mitochondria-independent ROS production via NADPH and xanthine oxidase activation. Cell Death Dis. 2014; 5:e1442. PMC: 4649505. DOI: 10.1038/cddis.2014.390. View

13.

Forth W, Adam O . Uptake of oxygen from the intestine-- experiments with rabbits. Eur J Med Res. 2001; 6(11):488-92. View

14.

Rusina E, Bernard C, Williamson A . The Kainic Acid Models of Temporal Lobe Epilepsy. eNeuro. 2021; 8(2). PMC: 8174050. DOI: 10.1523/ENEURO.0337-20.2021. View

15.

Fang C, Tsai C, Shyong Y, Yang C, Li K, Lin Y . Effects of Highly Oxygenated Water in a Hyperuricemia Rat Model. J Healthc Eng. 2020; 2020:1323270. PMC: 7013358. DOI: 10.1155/2020/1323270. View

16.

Kwon H, Lee J, Park H, Shin J, Yin Y, Shin N . Vitamin E reduces spasms caused by prenatal stress by lowering calpain expression. Epilepsy Behav. 2020; 114(Pt A):107609. DOI: 10.1016/j.yebeh.2020.107609. View

17.

Piantadosi C . "Oxygenated" water and athletic performance. Br J Sports Med. 2006; 40(9):740-1. PMC: 2564385. DOI: 10.1136/bjsm.2006.028936. View

18.

Chen Z, Brodie M, Liew D, Kwan P . Treatment Outcomes in Patients With Newly Diagnosed Epilepsy Treated With Established and New Antiepileptic Drugs: A 30-Year Longitudinal Cohort Study. JAMA Neurol. 2017; 75(3):279-286. PMC: 5885858. DOI: 10.1001/jamaneurol.2017.3949. View

19.

McGarry T, Biniecka M, Veale D, Fearon U . Hypoxia, oxidative stress and inflammation. Free Radic Biol Med. 2018; 125:15-24. DOI: 10.1016/j.freeradbiomed.2018.03.042. View

20.

Mukandala G, Tynan R, Lanigan S, OConnor J . The Effects of Hypoxia and Inflammation on Synaptic Signaling in the CNS. Brain Sci. 2016; 6(1). PMC: 4810176. DOI: 10.3390/brainsci6010006. View