The Antagonism of Neuropeptide Y Type I Receptor (Y1R) Reserves the Viability of Bone Marrow Stromal Cells in the Milieu of Osteonecrosis of Femoral Head (ONFH)

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2022 Nov 26

PMID 36428510

Authors

Jih-Yang Ko

Feng-Sheng Wang

Sung-Hsiung Chen

Re-Wen Wu

Chieh-Cheng Hsu

Shu-Jui Kuo

Affiliations

Soon will be listed here.

Abstract

Neuropeptide Y (NPY)-Y1 receptor (Y1R) signaling is known to negatively affect bone anabolism. Our study aimed at investigating the impact of NPY-Y1R signaling in the pathogenesis of glucocorticoid-related osteonecrosis of the femoral head (ONFH). Femoral heads were retrieved from 20 patients with and without ONFH, respectively. The bone marrow stromal cells (BMSCs) from ONFH femoral heads were treated with Y1R agonists and antagonists for subsequent analysis. We showed that the local NPY expression level was lower in ONFH heads. The Y1R agonists and antagonists disturb and facilitate the survival of BMSCs. The transcription of stromal derived factor-1 (SDF-1) was enhanced by Y1R antagonists. Our study showed that the local NPY expression level was lower in ONFH heads. Y1R antagonists facilitate the survival of BMSCs and stimulate the transcription of SDF-1 by BMSCs. These findings shed light on the role of NPY-Y1R signaling in the pathogenesis of ONFH.

Citing Articles

Dickkopf-1 (DKK1) blockade mitigates osteogenesis imperfecta (OI) related bone disease.

Ko J, Wang F, Lian W, Yang F, Chen J, Huang P Mol Med. 2024; 30(1):66.

PMID: 38773377 PMC: 11106911. DOI: 10.1186/s10020-024-00838-3.

Micro Ribonucleic Acid-29a (miR-29a) Antagonist Normalizes Bone Metabolism in Osteogenesis Imperfecta (OI) Mice Model.

Ko J, Wang F, Chen S, Kuo S Biomedicines. 2023; 11(2).

PMID: 36831000 PMC: 9953572. DOI: 10.3390/biomedicines11020465.

References

Hosogane N, Huang Z, Rawlins B, Liu X, Boachie-Adjei O, Boskey A . Stromal derived factor-1 regulates bone morphogenetic protein 2-induced osteogenic differentiation of primary mesenchymal stem cells. Int J Biochem Cell Biol. 2010; 42(7):1132-41. PMC: 2992806. DOI: 10.1016/j.biocel.2010.03.020. View

Lin R, Sung P, Wu C, Tu Y, Lu Y, Yip H . Decreased Ankyrin Expression Is Associated with Repressed eNOS Signaling, Cell Proliferation, and Osteogenic Differentiation in Osteonecrosis of the Femoral Head. J Bone Joint Surg Am. 2022; 104(Suppl 2):2-12. DOI: 10.2106/JBJS.20.00465. View

Sousa D, Baldock P, Enriquez R, Zhang L, Sainsbury A, Lamghari M . Neuropeptide Y Y1 receptor antagonism increases bone mass in mice. Bone. 2012; 51(1):8-16. DOI: 10.1016/j.bone.2012.03.020. View

Chen Y, Miao Y, Liu K, Xue F, Zhu B, Zhang C . Evolutionary course of the femoral head osteonecrosis: Histopathological - radiologic characteristics and clinical staging systems. J Orthop Translat. 2022; 32:28-40. PMC: 9072800. DOI: 10.1016/j.jot.2021.07.004. View

Ko J, Chen S, Wu R, Wu K, Hsu C, Kuo S . Decreased Expression of Leptin among Patients with Shoulder Stiffness. Life (Basel). 2022; 12(10). PMC: 9605091. DOI: 10.3390/life12101588. View

Chen Q, Zhang Y . The Role of NPY in the Regulation of Bone Metabolism. Front Endocrinol (Lausanne). 2022; 13:833485. PMC: 8902412. DOI: 10.3389/fendo.2022.833485. View

Konarski W, Pobozy T, Sliwczynski A, Kotela I, Krakowiak J, Hordowicz M . Avascular Necrosis of Femoral Head-Overview and Current State of the Art. Int J Environ Res Public Health. 2022; 19(12). PMC: 9223442. DOI: 10.3390/ijerph19127348. View

Lee N, Nguyen A, Enriquez R, Doyle K, Sainsbury A, Baldock P . Osteoblast specific Y1 receptor deletion enhances bone mass. Bone. 2010; 48(3):461-7. DOI: 10.1016/j.bone.2010.10.174. View

Zhu W, Boachie-Adjei O, Rawlins B, Frenkel B, Boskey A, Ivashkiv L . A novel regulatory role for stromal-derived factor-1 signaling in bone morphogenic protein-2 osteogenic differentiation of mesenchymal C2C12 cells. J Biol Chem. 2007; 282(26):18676-85. DOI: 10.1074/jbc.M610232200. View

10.

Baldock P, Lee N, Driessler F, Lin S, Allison S, Stehrer B . Neuropeptide Y knockout mice reveal a central role of NPY in the coordination of bone mass to body weight. PLoS One. 2009; 4(12):e8415. PMC: 2794533. DOI: 10.1371/journal.pone.0008415. View

11.

Lee N, Doyle K, Sainsbury A, Enriquez R, Hort Y, Riepler S . Critical role for Y1 receptors in mesenchymal progenitor cell differentiation and osteoblast activity. J Bone Miner Res. 2010; 25(8):1736-47. DOI: 10.1002/jbmr.61. View

12.

Baig S, Baig M . Osteonecrosis of the Femoral Head: Etiology, Investigations, and Management. Cureus. 2018; 10(8):e3171. PMC: 6197539. DOI: 10.7759/cureus.3171. View

13.

Liu S, Jin D, Wu J, Xu Z, Fu S, Mei G . Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro. Neuropeptides. 2015; 56:105-13. DOI: 10.1016/j.npep.2015.12.008. View

14.

Elgaz S, Bonig H, Bader P . Mesenchymal stromal cells for osteonecrosis. J Transl Med. 2020; 18(1):399. PMC: 7576732. DOI: 10.1186/s12967-020-02565-9. View

15.

Rubin J, Murphy T, Fan X, Goldschmidt M, Taylor W . Activation of extracellular signal-regulated kinase is involved in mechanical strain inhibition of RANKL expression in bone stromal cells. J Bone Miner Res. 2002; 17(8):1452-60. DOI: 10.1359/jbmr.2002.17.8.1452. View

16.

Zhang Y, Chen C, Liu Y, Rao S, Tan Y, Qian Y . Neuronal Induction of Bone-Fat Imbalance through Osteocyte Neuropeptide Y. Adv Sci (Weinh). 2021; 8(24):e2100808. PMC: 8693044. DOI: 10.1002/advs.202100808. View

17.

Oberto A, Bertocchi I, Longo A, Bonzano S, Paterlini S, Meda C . Hypothalamic NPY-Y1R Interacts with Gonadal Hormones in Protecting Female Mice against Obesity and Neuroinflammation. Int J Mol Sci. 2022; 23(11). PMC: 9180984. DOI: 10.3390/ijms23116351. View

18.

Wu J, Jiang N, Yu B . Mechanisms of action of neuropeptide Y on stem cells and its potential applications in orthopaedic disorders. World J Stem Cells. 2020; 12(9):986-1000. PMC: 7524693. DOI: 10.4252/wjsc.v12.i9.986. View

19.

Sousa D, Conceicao F, Silva D, Leitao L, Neto E, Alves C . Ablation of Y1 receptor impairs osteoclast bone-resorbing activity. Sci Rep. 2016; 6:33470. PMC: 5028844. DOI: 10.1038/srep33470. View

20.

Li Z, Lin H, Shi S, Su K, Zheng G, Gao S . Controlled and Sequential Delivery of Stromal Derived Factor-1 α (SDF-1α) and Magnesium Ions from Bifunctional Hydrogel for Bone Regeneration. Polymers (Basel). 2022; 14(14). PMC: 9315491. DOI: 10.3390/polym14142872. View