Temperature and Humidity Regulate Sporulation of That Is Associated with Pathogenicity in Cucumber ( L.)

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2022 Nov 24

PMID 36421389

Authors

Qian Zhao

Yanxia Shi

Yikai Wang

Xuewen Xie

Lei Li

Tengfei Fan

Liyun Guo

Ali Chai

Baoju Li

Affiliations

Soon will be listed here.

Abstract

Cucumber target leaf spot, caused by , is an emerging disease with a high incidence that causes severe damage to cucumbers on a global scale. Therefore, efforts need to be undertaken to limit the spread and infection of this pathogen, preferably by using environmentally friendly methods. In this study, the effects of temperature and moisture on the sporulation of . were investigated in vitro and in vivo. The novelty of our study refers to the observation of spore production and size as well as the revelation of a correlation between spore size and virulence. On potato dextrose agar (PDA) and cucumber-leaf extract agar (CEA), temperature played a critical role in spore production, which was strongly influenced by both temperature and moisture on detached leaves and cucumber seedlings. Maximum spore production was found at 30 °C on PDA and 25 °C on CEA, cucumber detached leaves and living plants. Lower spore productions were observed with a stepwise change of 5 °C. In addition, the largest spore production was found at 100% relative humidity (RH) in comparison to the other tested moisture. Moreover, moisture was found to be the most important factor affecting spore size, accounting for 83.09-84.86% of the total variance in length and 44.72-73.10% of the total variance in width. The longest-narrowest spores were formed at 100% RH, and the shortest-widest spores were formed at 75% RH. Furthermore, the result showed that larger spores of were more virulent and small spores were avirulent. Our findings will contribute to the development of new strategies for the effective alleviation and control of cucumber target leaf spot.

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