Effects of Oxygen Transference on Protease Production by CBMAI 1528 in a Stirred Tank Bioreactor

Overview

Journal Bioengineering (Basel)

Date 2022 Nov 24

PMID 36421095

Authors

Suellen Machado

Valker Feitosa

Omar Pillaca-Pullo

Luciana Lario

Lara Sette

Adalberto Pessoa Jr

Harley Alves

Affiliations

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Abstract

Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling-up processes. This is the first study that evaluates the influence of oxygen transference () on the protease production of yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under values from 18 to 135 h. The results show that (121 h) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), (0.14 h) and (0.484 g/g). In conclusion, showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by . In this sense, our results could be used for further industrial investment.

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