Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) Against Causing Aspergillosis: Ultrastructure Study

Overview

Journal J Funct Biomater

Specialty Biomedical Engineering

Date 2022 Nov 22

PMID 36412883

Authors

Amr H Hashem

Ebrahim Saied

Basma H Amin

Fatimah O Alotibi

Abdulaziz A Al-Askar

Amr A Arishi

Fathy M Elkady

Mostafa A Elbahnasawy

Affiliations

Soon will be listed here.

Abstract

Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common , including , , , and at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against , , , and were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall's integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of can be used as a promising antifungal agent against species causing Aspergillosis.

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