Regulatory Effects of Var. Li. Polysaccharides on Immune System and Gut Microbiota in Cyclophosphamide-induced Mice

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2022 Nov 21

PMID 36406390

Authors

Ming Zhao

Wei Shi

Xijun Chen

Yanfang Liu

Yan Yang

Xianghui Kong

Affiliations

Soon will be listed here.

Abstract

The immuno-regulating potential of edible fungus polysaccharides has gained more and more attention. However, there is little information about the study of var. Li. polysaccharides regulating immunomodulatory activity. The objective of this work to analyze the immunomodulatory activity and the mechanism of var. Li. polysaccharides supplementation in an immunosuppressed mice model induced by cyclophosphamide. The effects of var. Li. polysaccharides on immune system including immune organ indices, immunoglobulin contents, and inflammation cytokines in immunosuppressed mice were determined. In addition, the regulatory effects of var. Li. polysaccharides on the gut microbiota and their metabolites were analyzed. Results showed that var. Li. polysaccharides significantly elevated immune organ indexes, remarkably enhanced the levels of immunoglobulin A (IgA), IgG and IgM in serum and secretory IgA (sIgA) in the intestinal mucosa, conspicuously stimulated the levels of tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), IL-4, and IL-10 in the serum. var. Li. polysaccharides also could restore gut microbiota to the pattern that is similar with that of the control group with increase of the relative abundances of short-chain fatty acids (SCFAs)-producing bacteria. Furthermore, the content of SCFAs were increased after var. Li. polysaccharides supplementation. This study provides useful information for applications of var. Li. polysaccharides in immune-regulated foods and medicine.

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