Relationships of Circulating CD4 T Cell Subsets and Cytokines with the Risk of Relapse in Patients with Crohn's Disease

Background And Aims: We aimed to analyze circulating CD4 T cell subsets and cytokines during the course of Crohn's disease (CD).

Methods And Results: CD4 T cell subsets, ultrasensitive C-reactive protein (usCRP), and various serum cytokines (IL-6, IL-8, IL-10, IL-13, IL-17A, IL-23, TNFα, IFNγ, and TGFβ) were prospectively monitored every 3 months for 1 year, using multicolor flow cytometry and an ultrasensitive Erenna method in CD patients in remission at inclusion. Relapse occurred in 35 out of the 113 consecutive patients (31%). For patients in remission within 4 months prior to relapse and at the time of relapse, there was no significant difference in Th1, Th17, Treg, and double-positive CD4 T cell subsets co-expressing either IFNγ and FOXP3, IL-17A and FOXP3, or IFNγ and IL-17A. On the contrary, in patients who remained in remission, the mean frequency and number of double-positive IL-17AFOXP3 CD4 T cells and the level of usCRP were significantly higher ( ≤ 0.01) 1 to 4 months prior to relapse. At the time of relapse, only the IL-6 and usCRP levels were significantly higher ( ≤ 0.001) compared with those patients in remission. On multivariate analysis, a high number of double-positive IL-17AFOXP3 CD4 T cells (≥1.4 cells/mm3) and elevated serum usCRP (≥3.44 mg/L) were two independent factors associated with risk of relapse.

Conclusions: Detection of circulating double-positive FOXP3IL-17A CD4 T cell subsets supports that T cell plasticity may reflect the inflammatory context of Crohn's disease. Whether this subset contributes to the pathogenesis of CD relapse needs further studies.