Genome-wide Scan for Potential CD4+ T-cell Vaccine Candidates in by Exploiting Reverse Vaccinology and Evolutionary Information

is a globally emerging fungal pathogen responsible for causing nosocomial outbreaks in healthcare associated settings. It is known to cause infection in all age groups and exhibits multi-drug resistance with high potential for horizontal transmission. Because of this reason combined with limited therapeutic choices available, infection has been acknowledged as a potential risk for causing a future pandemic, and thus seeking a promising strategy for its treatment is imperative. Here, we combined evolutionary information with reverse vaccinology approach to identify novel epitopes for vaccine design that could elicit CD4+ T-cell responses against To this end, we extensively scanned the family of proteins encoded by genome. In addition, a pathogen may acquire substitutions in epitopes over a period of time which could cause its escape from the immune response thus rendering the vaccine ineffective. To lower this possibility in our design, we eliminated all rapidly evolving genes of with positive selection. We further employed highly conserved regions of multiple strains and identified two immunogenic and antigenic T-cell epitopes that could generate the most effective immune response against The antigenicity scores of our predicted vaccine candidates were calculated as 0.85 and 1.88 where 0.5 is the threshold for prediction of fungal antigenic sequences. Based on our results, we conclude that our vaccine candidates have the potential to be successfully employed for the treatment of infection. However, experiments are imperative to further demonstrate the efficacy of our design.