Clinical Prospective Assessment of Genotoxic Effects of Dental Implants in Gingival Epithelial Cells

Overview

Journal Acta Stomatol Croat

Specialty Dentistry

Date 2022 Nov 16

PMID 36382208

Authors

Mirna Radovic

Lidia Gavic

Daniel Jerkovic

Davor Zeljezic

Jasna Puizina

Ivan Srzentic

Ema Puizina Mladinic

Antonija Tadin

Affiliations

Soon will be listed here.

Abstract

Objectives: Although titanium-based implants are considered bioinert, it has been found that they are subject to corrosion and wear. This study aimed to evaluate the cytotoxic and genotoxic potential of two implant systems in gingival epithelial cells.

Material And Methods: Gingival swabs were taken three times from 91 subjects. The first swab was taken before dental implant placement, the second swab 90 days after dental implant placement and the third swab 21 days following the healing abutment placement. DNA damage was analyzed using the micronucleus test. Tested dental implants with corresponding healing abutments were Ankylos and Dentium SuperLine.

Results: Of all scored forms of cytogenetic damage in gingival cells of individuals after implementation of tested dental implant systems, only an increase in the number of binucleated cells (P ≤ 0.001) was significant in contrast to control values for both tested implant systems, 90 days after dental implant placement and 21 days following the healing abutment placement.

Conclusion: It may be concluded that there are no titanium-based implant dependent cytogenetic damage in gingival epithelial cells. A slight increase in cytogenetic damage has been observed but it is of no biological relevance and might be associated with healing abutment induced effect.

Citing Articles

Assessment of Cytotoxic and Genotoxic Effect of Modern Dental Materials .

Trutina Gavran M, Zeljezic D, Vranic L, Negovetic Vranic D, Grabarevic L, Juric-Kacunaric D Acta Stomatol Croat. 2023; 57(3):216-228.

PMID: 37808410 PMC: 10557110. DOI: 10.15644/asc57/3/2.

Porphyromonas Gingivalis in Different Peri-Implant Conditions: a Pilot Cross - Sectional Study.

Savcic N, Henjas D, Jezdic M, dinic Krasavcevic A, Milinkovic I Acta Stomatol Croat. 2023; 56(4):387-394.

PMID: 36713273 PMC: 9873002. DOI: 10.15644/asc56/4/5.

References

Song B, Zhang Y, Chen L, Zhou T, Huang W, Zhou X . The role of Toll-like receptors in periodontitis. Oral Dis. 2016; 23(2):168-180. DOI: 10.1111/odi.12468. View

Souza J, Barbosa S, Ariza E, Henriques M, Teughels W, Ponthiaux P . How do titanium and Ti6Al4V corrode in fluoridated medium as found in the oral cavity? An in vitro study. Mater Sci Eng C Mater Biol Appl. 2014; 47:384-93. DOI: 10.1016/j.msec.2014.11.055. View

Kumazawa R, Watari F, Takashi N, Tanimura Y, Uo M, Totsuka Y . Effects of Ti ions and particles on neutrophil function and morphology. Biomaterials. 2002; 23(17):3757-64. DOI: 10.1016/s0142-9612(02)00115-1. View

Eskan M, Benakanakere M, Rose B, Zhang P, Zhao J, Stathopoulou P . Interleukin-1beta modulates proinflammatory cytokine production in human epithelial cells. Infect Immun. 2008; 76(5):2080-9. PMC: 2346720. DOI: 10.1128/IAI.01428-07. View

Broggini N, McManus L, Hermann J, Medina R, Schenk R, Buser D . Peri-implant inflammation defined by the implant-abutment interface. J Dent Res. 2006; 85(5):473-8. DOI: 10.1177/154405910608500515. View

Harris B, Kohles S . Effects of mechanical and thermal fatigue on dental drill performance. Int J Oral Maxillofac Implants. 2002; 16(6):819-26. View

Bressan E, Ferroni L, Gardin C, Bellin G, Sbricoli L, Sivolella S . Metal Nanoparticles Released from Dental Implant Surfaces: Potential Contribution to Chronic Inflammation and Peri-Implant Bone Loss. Materials (Basel). 2019; 12(12). PMC: 6630980. DOI: 10.3390/ma12122036. View

Tavares J, Cornelio D, da Silva N, de Moura C, de Queiroz J, Sa J . Effect of titanium surface modified by plasma energy source on genotoxic response in vitro. Toxicology. 2009; 262(2):138-45. DOI: 10.1016/j.tox.2009.05.020. View

Gavic L, Gorseta K, Buterin A, Glavina D, Zeljezic D, Tadin A . Assessment of Cytotoxic and Genotoxic Effect of Fissure Sealants in Buccal Epithelial Cells. Acta Stomatol Croat. 2021; 55(1):10-17. PMC: 8033626. DOI: 10.15644/asc55/1/2. View

10.

Ribeiro A, Gemini-Piperni S, Travassos R, Lemgruber L, Silva R, Rossi A . Trojan-Like Internalization of Anatase Titanium Dioxide Nanoparticles by Human Osteoblast Cells. Sci Rep. 2016; 6:23615. PMC: 4810327. DOI: 10.1038/srep23615. View

11.

Karci B, Oncu E . Comparison of Osteoimmunological and Microbiological Parameters of Extra Short and Longer Implants Loaded in the Posterior Mandible: A Split Mouth Randomized Clinical Study. Acta Stomatol Croat. 2021; 55(3):238-247. PMC: 8514233. DOI: 10.15644/asc55/3/1. View

12.

Clark D, Levin L . Dental implant management and maintenance: How to improve long-term implant success?. Quintessence Int. 2016; 47(5):417-23. DOI: 10.3290/j.qi.a35870. View

13.

Li J, Chen J, Kirsner R . Pathophysiology of acute wound healing. Clin Dermatol. 2007; 25(1):9-18. DOI: 10.1016/j.clindermatol.2006.09.007. View

14.

Allsobrook O, Leichter J, Holborrow D, Swain M . Descriptive study of the longevity of dental implant surgery drills. Clin Implant Dent Relat Res. 2011; 13(3):244-54. DOI: 10.1111/j.1708-8208.2009.00205.x. View

15.

Carvalho A, Queiroz T, Okamoto R, Margonar R, Garcia Jr I, Magro Filho O . Evaluation of bone heating, immediate bone cell viability, and wear of high-resistance drills after the creation of implant osteotomies in rabbit tibias. Int J Oral Maxillofac Implants. 2011; 26(6):1193-201. View

16.

Karahalil B, Kadioglu E, Tuzuner-Oncul A, Cimen E, Emerce E, Kisnisci R . Micronucleus assay assessment of possible genotoxic effects in patients treated with titanium alloy endosseous implants or miniplates. Mutat Res Genet Toxicol Environ Mutagen. 2013; 760:70-2. DOI: 10.1016/j.mrgentox.2013.10.005. View

17.

Okuda-Shimazaki J, Takaku S, Kanehira K, Sonezaki S, Taniguchi A . Effects of titanium dioxide nanoparticle aggregate size on gene expression. Int J Mol Sci. 2010; 11(6):2383-92. PMC: 2904923. DOI: 10.3390/ijms11062383. View

18.

Ribeiro D, Matsumoto M, Padovan L, Marques M, Salvadori D . Genotoxicity of corrosion eluates obtained from endosseous implants. Implant Dent. 2007; 16(1):101-9. DOI: 10.1097/ID.0b013e3180327663. View

19.

Sidambe A . Biocompatibility of Advanced Manufactured Titanium Implants-A Review. Materials (Basel). 2017; 7(12):8168-8188. PMC: 5456424. DOI: 10.3390/ma7128168. View

20.

Kim K, Eo M, Thi Hoang Nguyen T, Kim S . General review of titanium toxicity. Int J Implant Dent. 2019; 5(1):10. PMC: 6409289. DOI: 10.1186/s40729-019-0162-x. View