TRNA-derived Fragment TRF-1001: A Novel Anti-angiogenic Factor in Pathological Ocular Angiogenesis

Overview

Journal Mol Ther Nucleic Acids

Publisher Cell Press

Date 2022 Nov 16

PMID 36381574

Authors

Qin Jiang

Yan Ma

Ya Zhao

Mu-Di Yao

Yan Zhu

Qiu-Yang Zhang

Biao Yan

Affiliations

Soon will be listed here.

Abstract

Transfer RNA-derived fragments (tRFs) are a novel class of non-coding RNA transcripts and play important roles in several physiological/pathological processes. However, the role of tRFs in ocular angiogenesis remains elusive. Herein, we investigate whether the intervention of tRF-1001 expression could suppress pathological ocular angiogenesis. The results show that the levels of tRF-1001 expression were reduced in the retinas of an oxygen-induced retinopathy (OIR) model, choroidal neovascularization model, and endothelial sprouting model . Increased tRF-1001 expression could suppress ocular angiogenesis and endothelial sprouting and reduce endothelial migration, specification, and sprouting . Mechanistically, tRF-1001 regulated endothelial angiogenic effects via tRF-1001/METTL3/RBPJ-MAML1 signaling. The levels of tRF-1001 expression were downregulated in the aqueous humor of age-related macular degeneration (AMD) patients. tRF-1001 upregulation could suppress AMD aqueous humor-induced endothelial sprouting and pathological angiogenesis. Collectively, tRF-1001 acts as an anti-angiogenic factor during ocular angiogenesis. Targeting tRF-1001-mediated signaling is a therapeutic option for ocular neovascular diseases.

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