Studying Hepatitis Virus-Host Interactions in Patient Liver Biopsies

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2022 Nov 11

PMID 36366588

Authors

Aleksei Suslov

Markus H Heim

Stefan Wieland

Affiliations

Soon will be listed here.

Abstract

Infectious diseases are a major contributor to human suffering and the associated socioeconomic burden worldwide. A better understanding of human pathogen-host interactions is a prerequisite for the development of treatment strategies aimed at combatting human pathogen-induced diseases. Model systems that faithfully recapitulate the pathogen-host interactions in humans are critical to gain meaningful insight. Unfortunately, such model systems are not yet available for a number of pathogens. The strict tropism of the hepatitis B (HBV) and C (HCV) viruses for the human liver has made it difficult to study their virus-host interactions during the natural history of these infections. In this case, surplus liver biopsy tissue donated by patients provides an opportunity to obtain a snapshot of the phenomenological and molecular aspects of the human liver of chronically HCV or HBV-infected patients. In this review, we will briefly summarize our own efforts over the years to advance our knowledge of the virus-host interactions during the natural history of chronic HCV and HBV infection.

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References

Chen L, Borozan I, Feld J, Sun J, Tannis L, Coltescu C . Hepatic gene expression discriminates responders and nonresponders in treatment of chronic hepatitis C viral infection. Gastroenterology. 2005; 128(5):1437-44. DOI: 10.1053/j.gastro.2005.01.059. View

Liu Y, Maya S, Ploss A . Animal Models of Hepatitis B Virus Infection-Success, Challenges, and Future Directions. Viruses. 2021; 13(5). PMC: 8146732. DOI: 10.3390/v13050777. View

Godoy P, Hewitt N, Albrecht U, Andersen M, Ansari N, Bhattacharya S . Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Arch Toxicol. 2013; 87(8):1315-530. PMC: 3753504. DOI: 10.1007/s00204-013-1078-5. View

Spearman C, Dusheiko G, Hellard M, Sonderup M . Hepatitis C. Lancet. 2019; 394(10207):1451-1466. DOI: 10.1016/S0140-6736(19)32320-7. View

Fisicaro P, Valdatta C, Boni C, Massari M, Mori C, Zerbini A . Early kinetics of innate and adaptive immune responses during hepatitis B virus infection. Gut. 2009; 58(7):974-82. DOI: 10.1136/gut.2008.163600. View

Tabata K, Neufeldt C, Bartenschlager R . Hepatitis C Virus Replication. Cold Spring Harb Perspect Med. 2019; 10(3). PMC: 7050578. DOI: 10.1101/cshperspect.a037093. View

Allweiss L, Dandri M . Experimental in vitro and in vivo models for the study of human hepatitis B virus infection. J Hepatol. 2016; 64(1 Suppl):S17-S31. DOI: 10.1016/j.jhep.2016.02.012. View

. EASL 2017 Clinical Practice Guidelines on the management of hepatitis B virus infection. J Hepatol. 2017; 67(2):370-398. DOI: 10.1016/j.jhep.2017.03.021. View

Heim M . 25 years of interferon-based treatment of chronic hepatitis C: an epoch coming to an end. Nat Rev Immunol. 2013; 13(7):535-42. DOI: 10.1038/nri3463. View

10.

Boldanova T, Suslov A, Heim M, Necsulea A . Transcriptional response to hepatitis C virus infection and interferon-alpha treatment in the human liver. EMBO Mol Med. 2017; 9(6):816-834. PMC: 5452008. DOI: 10.15252/emmm.201607006. View

11.

Allweiss L, Strick-Marchand H . In-vitro and in-vivo models for hepatitis B cure research. Curr Opin HIV AIDS. 2020; 15(3):173-179. DOI: 10.1097/COH.0000000000000616. View

12.

Tu T, Budzinska M, Shackel N, Urban S . HBV DNA Integration: Molecular Mechanisms and Clinical Implications. Viruses. 2017; 9(4). PMC: 5408681. DOI: 10.3390/v9040075. View

13.

Bellecave P, Sarasin-Filipowicz M, Donze O, Kennel A, Gouttenoire J, Meylan E . Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system. Hepatology. 2010; 51(4):1127-36. DOI: 10.1002/hep.23426. View

14.

Suslov A, Wieland S, Menne S . Modulators of innate immunity as novel therapeutics for treatment of chronic hepatitis B. Curr Opin Virol. 2018; 30:9-17. PMC: 5988934. DOI: 10.1016/j.coviro.2018.01.008. View

15.

Heim M, Bochud P, George J . Host - hepatitis C viral interactions: The role of genetics. J Hepatol. 2016; 65(1 Suppl):S22-S32. DOI: 10.1016/j.jhep.2016.07.037. View

16.

Dill M, Makowska Z, Trincucci G, Gruber A, Vogt J, Filipowicz M . Pegylated IFN-α regulates hepatic gene expression through transient Jak/STAT activation. J Clin Invest. 2014; 124(4):1568-81. PMC: 3973080. DOI: 10.1172/JCI70408. View

17.

Wieland S . The chimpanzee model for hepatitis B virus infection. Cold Spring Harb Perspect Med. 2015; 5(6). PMC: 4448699. DOI: 10.1101/cshperspect.a021469. View

18.

Dill M, Duong F, Vogt J, Bibert S, Bochud P, Terracciano L . Interferon-induced gene expression is a stronger predictor of treatment response than IL28B genotype in patients with hepatitis C. Gastroenterology. 2010; 140(3):1021-31. DOI: 10.1053/j.gastro.2010.11.039. View

19.

Di Blasi D, Boldanova T, Mori L, Terracciano L, Heim M, De Libero G . Unique T-Cell Populations Define Immune-Inflamed Hepatocellular Carcinoma. Cell Mol Gastroenterol Hepatol. 2019; 9(2):195-218. PMC: 6957799. DOI: 10.1016/j.jcmgh.2019.08.004. View

20.

Dunn C, Peppa D, Khanna P, Nebbia G, Jones M, Brendish N . Temporal analysis of early immune responses in patients with acute hepatitis B virus infection. Gastroenterology. 2009; 137(4):1289-300. DOI: 10.1053/j.gastro.2009.06.054. View