Liposomal Formulation of Botanical Extracts May Enhance Yield Triggering PR Genes and Phenylpropanoid Pathway in Barley ()

Overview

Journal Plants (Basel)

Date 2022 Nov 11

PMID 36365426

Authors

Geza Hegedus

Barbara Kutasy

Marta Kiniczky

Kincso Decsi

Akos Juhasz

Agnes Nagy

Jozsef Peter Pallos

Eszter Virag

Affiliations

Soon will be listed here.

Abstract

This work aimed to study the plant conditioning effect and mode of action of a plant-based biostimulant used in organic farming. This new generation plant biostimulant, named ELICE16INDURES, is rich in plant bio-active ingredients containing eleven supercritical botanical extracts encapsulated in nano-scale liposomes. The dose-response (10 to 240 g ha) relationship was tested in a field population of autumn barley ( test crop, and underlying molecular mechanisms were studied. Applying nanotechnology, cell-identical nanoparticles may help the better uptake and delivery of active ingredients increasing resilience, vitality, and crop yield. The amount of harvested crops showed a significant increase of 27.5% and 39.9% interconnected to higher normalized difference vegetation index (NDVI) of 20% and 25% after the treatment of low and high dosages (20 and 240 g ha), respectively. Illumina NextSeq 550 sequencing, gene expression profiling, and KEGG-pathway analysis of outstanding dosages indicated the upregulation of pathogenesis-related (PR) and other genes-associated with induced resistance-which showed dose dependency as well.

Citing Articles

Evaluation of the Biostimulant Activity and Verticillium Wilt Protection of an Onion Extract in Olive Crops ().

Falcon-Pineiro A, Zaguirre-Martinez J, Ibanez-Hernandez A, Guillamon E, Santander K, Barrero-Dominguez B Plants (Basel). 2024; 13(17).

PMID: 39273983 PMC: 11397703. DOI: 10.3390/plants13172499.

Supplementation of the Plant Conditioner ELICE Vakcina Product with β-Aminobutyric Acid and Salicylic Acid May Lead to -Priming Signaling in Barley ().

Virag E, Kiniczky M, Kutasy B, Nagy A, Pallos J, Laczko L Plants (Basel). 2023; 12(12).

PMID: 37375933 PMC: 10305027. DOI: 10.3390/plants12122308.

'Garlic-lipo'4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat ().

Kutasy B, Kiniczky M, Decsi K, Kalman N, Hegedus G, Alfoldi Z Plants (Basel). 2023; 12(4).

PMID: 36840091 PMC: 9962754. DOI: 10.3390/plants12040743.

Natural immunity stimulation using ELICE16INDURES® plant conditioner in field culture of soybean.

Decsi K, Kutasy B, Hegedus G, Alfoldi Z, Kalman N, Nagy A Heliyon. 2023; 9(1):e12907.

PMID: 36691550 PMC: 9860300. DOI: 10.1016/j.heliyon.2023.e12907.

References

Singh P, Mukhopadhyay K . Comprehensive molecular dissection of TIFY Transcription factors reveal their dynamic responses to biotic and abiotic stress in wheat (Triticum aestivum L.). Sci Rep. 2021; 11(1):9739. PMC: 8102568. DOI: 10.1038/s41598-021-87722-w. View

Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011; 29(7):644-52. PMC: 3571712. DOI: 10.1038/nbt.1883. View

Kefauver S, Vicente R, Vergara-Diaz O, Fernandez-Gallego J, Kerfal S, Lopez A . Comparative UAV and Field Phenotyping to Assess Yield and Nitrogen Use Efficiency in Hybrid and Conventional Barley. Front Plant Sci. 2017; 8:1733. PMC: 5641326. DOI: 10.3389/fpls.2017.01733. View

KATZ , Thulke , Conrath . A benzothiadiazole primes parsley cells for augmented elicitation of defense responses . Plant Physiol. 1998; 117(4):1333-9. PMC: 34897. DOI: 10.1104/pp.117.4.1333. View

Kutasy B, Decsi K, Kiniczky M, Hegedus G, Virag E . Time-course gene expression profiling data of treated by supercritical CO garlic extract encapsulated in nanoscale liposomes. Data Brief. 2022; 42:108287. PMC: 9142676. DOI: 10.1016/j.dib.2022.108287. View

Robinson M, McCarthy D, Smyth G . edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2009; 26(1):139-40. PMC: 2796818. DOI: 10.1093/bioinformatics/btp616. View

Langmead B, Salzberg S . Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012; 9(4):357-9. PMC: 3322381. DOI: 10.1038/nmeth.1923. View

Gomez-Ariza J, Campo S, Rufat M, Estopa M, Messeguer J, San Segundo B . Sucrose-mediated priming of plant defense responses and broad-spectrum disease resistance by overexpression of the maize pathogenesis-related PRms protein in rice plants. Mol Plant Microbe Interact. 2007; 20(7):832-42. DOI: 10.1094/MPMI-20-7-0832. View

Luo X, Tian T, Feng L, Yang X, Li L, Tan X . Pathogenesis-related protein 1 suppresses oomycete pathogen by targeting against AMPK kinase complex. J Adv Res. 2022; 43:13-26. PMC: 9811325. DOI: 10.1016/j.jare.2022.02.002. View

10.

Yang F, Jensen J, Svensson B, Jorgensen H, Collinge D, Finnie C . Analysis of early events in the interaction between Fusarium graminearum and the susceptible barley (Hordeum vulgare) cultivar Scarlett. Proteomics. 2010; 10(21):3748-55. DOI: 10.1002/pmic.201000243. View

11.

Mayer L, Bally M, Hope M, Cullis P . Techniques for encapsulating bioactive agents into liposomes. Chem Phys Lipids. 1986; 40(2-4):333-45. DOI: 10.1016/0009-3084(86)90077-0. View

12.

Chaman M, Copaja S, Argandona V . Relationships between salicylic acid content, phenylalanine ammonia-lyase (PAL) activity, and resistance of barley to aphid infestation. J Agric Food Chem. 2003; 51(8):2227-31. DOI: 10.1021/jf020953b. View

13.

Anders S, Pyl P, Huber W . HTSeq--a Python framework to work with high-throughput sequencing data. Bioinformatics. 2014; 31(2):166-9. PMC: 4287950. DOI: 10.1093/bioinformatics/btu638. View

14.

Conrath U, Pieterse C, Mauch-Mani B . Priming in plant-pathogen interactions. Trends Plant Sci. 2002; 7(5):210-6. DOI: 10.1016/s1360-1385(02)02244-6. View

15.

Vargas-Hernandez M, Macias-Bobadilla I, Guevara-Gonzalez R, Romero-Gomez S, Rico-Garcia E, Ocampo-Velazquez R . Plant Hormesis Management with Biostimulants of Biotic Origin in Agriculture. Front Plant Sci. 2017; 8:1762. PMC: 5645530. DOI: 10.3389/fpls.2017.01762. View

16.

Rouphael Y, Colla G . Editorial: Biostimulants in Agriculture. Front Plant Sci. 2020; 11:40. PMC: 7010726. DOI: 10.3389/fpls.2020.00040. View

17.

Santelia D, Zeeman S . Progress in Arabidopsis starch research and potential biotechnological applications. Curr Opin Biotechnol. 2010; 22(2):271-80. DOI: 10.1016/j.copbio.2010.11.014. View

18.

Choudhary D, Prakash A, Johri B . Induced systemic resistance (ISR) in plants: mechanism of action. Indian J Microbiol. 2012; 47(4):289-97. PMC: 3450033. DOI: 10.1007/s12088-007-0054-2. View

19.

Burketova L, Trda L, Ott P, Valentova O . Bio-based resistance inducers for sustainable plant protection against pathogens. Biotechnol Adv. 2015; 33(6 Pt 2):994-1004. DOI: 10.1016/j.biotechadv.2015.01.004. View

20.

Caruso C, Chilosi G, Leonardi L, Bertini L, Magro P, Buonocore V . A basic peroxidase from wheat kernel with antifungal activity. Phytochemistry. 2001; 58(5):743-50. DOI: 10.1016/s0031-9422(01)00226-6. View