Rapid Identification of Paeoniae Radix and Moutan Radicis Cortex Using a SCAR Marker-Based Conventional PCR Assay

Overview

Journal Plants (Basel)

Date 2022 Nov 11

PMID 36365322

Authors

Wook Jin Kim

Sumin Noh

Goya Choi

Byeong Cheol Moon

Affiliations

Soon will be listed here.

Abstract

Paeoniae Radix is a herbal medicine prepared from the dried roots of , subsp. , and . Although the herbal medicines prepared from these species are morphologically similar, they have different pharmacological effects depending on how they are processed. In addition, is more expensive than other spp. in the Korean herbal market. Although there is a clear difference between the Korean and Chinese pharmacopeias of Paeoniae Radix, the processed roots of and subsp. are commonly used indiscriminately in the herbal market. Moreover, , an allied genus of , is prescribed as Moutan Radicis Cortex. Therefore, accurate taxonomic identification of plant species is vital for quality assurance. A genetic assay is a reliable tool for accurately discriminating species in processed herbal medicines. To develop a genetic assay for the identification of four species (, subsp. , , and ), we analyzed the sequences of two DNA barcoding regions, internal transcribed spacer and L. A conventional PCR assay was established in this study for simple and rapid species identification using sequence characterized amplified region (SCAR) markers based on arbitrary nucleotide-containing primers. This assay was verified to be species specific and highly sensitive and could be applied to species identification at an affordable rate.

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