A Novel RHS1 Locus in Rice Attributes Seed-Pod Shattering by the Regulation of Endogenous S-Nitrosothiols

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Nov 11

PMID 36362013

Authors

Bong-Gyu Mun

Muhammad Shahid

Gang Sub Lee

Adil Hussain

Byung-Wook Yun

Affiliations

Soon will be listed here.

Abstract

Seed or pod shattering in rice ( sativa) is considered to be one of the major factors involved in the domestication of rice as a crop. High seed shattering results in significant yield losses. In this study, we characterize the () that corresponds to the locus from a greenhouse screen, involving 145 Ac/Ds transposon mutant rice lines. The knockout mutant line exhibited a significantly high shattering of grains in comparison to the wild-type plants. The exogenous application of nitric oxide (NO) resulted in a significant reduction in the expression of in wild-type rice plants. The absence of , which encodes a putative armadillo/beta-catenin repeat family protein, resulted in high sensitivity of the plants to nitrosative stress. Interestingly, the basal expression levels of and genes (transcription factors that regulate seed-pod shattering in rice) were significantly lower in these plants than in wild-type plants; however, nitrosative stress negatively regulated the expression of and in both WT and plants, but positively regulated expression in plants alone. The expression levels of genes responsible for NO production (, and ) were lower in plants than in WT plants under normal conditions. However, under nitrosative stress, the expression of significantly increased in plants. The expression of (a negative regulator of seed shattering in rice) was significantly lower in plants, and we found that expression was correlated with -nitrosothiol (SNO) alteration in Interestingly , a rice mutant with high SNO levels, exhibited low seed shattering, whereas resulted in low SNO levels with high seed shattering. Therefore, is a novel gene that negatively regulates the shattering trait in rice via regulation of endogenous SNO levels. However, the molecular mechanisms involved in the control of -mediated regulation of seed shattering and its interaction with nitric oxide and involvement in plant defense need to be investigated further.

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