Mimicking Pseudo-Virion Interactions with Abiotic Surfaces: Deposition of Polymer Nanoparticles with Albumin Corona

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2022 Nov 11

PMID 36359008

Authors

Malgorzata Nattich-Rak

Marta Sadowska

Maja Motyczynska

Zbigniew Adamczyk

Affiliations

Soon will be listed here.

Abstract

Adsorption of human serum albumin (HSA) molecules on negatively charged polystyrene microparticles was studied using the dynamic light scattering, the electrophoretic and the solution depletion methods involving atomic force microscopy. Initially, the physicochemical characteristics of the albumin comprising the hydrodynamic diameter, the zeta potential and the isoelectric point were determined as a function of pH. Analogous characteristics of the polymer particles were acquired, including their size and zeta potential. The formation of albumin corona on the particles was investigated in situ by electrophoretic mobility measurements. The size, stability and electrokinetic properties of the particles with the corona were also determined. The particle diameter was equal to 125 nm, which coincides with the size of the SARS-CoV-2 virion. The isoelectric point of the particles appeared at a pH of 5. The deposition kinetics of the particles was determined by atomic force microscopy (AFM) under diffusion and by quartz microbalance (QCM) under flow conditions. It was shown that the deposition rate at a gold sensor abruptly vanished with pH following the decrease in the zeta potential of the particles. It is postulated that the acquired results can be used as useful reference systems mimicking virus adsorption on abiotic surfaces.

Citing Articles

Deposition of Human-Serum-Albumin-Functionalized Spheroidal Particles on Abiotic Surfaces: Reference Kinetic Results for Bioparticles.

Nattich-Rak M, Sadowska M, Adamczyk Z, Basinska T, Mickiewicz D, Gadzinowski M Molecules. 2024; 29(14).

PMID: 39064983 PMC: 11279952. DOI: 10.3390/molecules29143405.

Quantifying Nanoparticle Layer Topography: Theoretical Modeling and Atomic Force Microscopy Investigations.

Adamczyk Z, Sadowska M, Nattich-Rak M Langmuir. 2023; 39(42):15067-15077.

PMID: 37824293 PMC: 10601541. DOI: 10.1021/acs.langmuir.3c02024.

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