The Antimicrobial, Antibiofilm, and Wound Healing Properties of Ethyl Acetate Crude Extract of an Endophytic Fungus Paecilomyces Sp. (AUMC 15510) in Earthworm Model

Overview

Journal Sci Rep

Specialty Science

Date 2022 Nov 10

PMID 36357560

Authors

Shimaa H Salem

Saad S El-Maraghy

Ahmed Y Abdel-Mallek

Mohamed A A Abdel-Rahman

Emad H M Hassanein

Osama A Al-Bedak

Fatma El-Zahraa A Abd El-Aziz

Affiliations

Soon will be listed here.

Abstract

The endophytic fungus Paecilomyces sp. (AUMC 15510) was isolated from healthy stem samples of the Egyptian medicinal plant Cornulaca monacantha. We used GC-MS and HPLC analysis to identify the bioactive constituents of ethyl acetate crude extract of Paecilomyces sp. (PsEAE). Six human microbial pathogens have been selected to evaluate the antimicrobial activity of PsEAE. Our data showed that the extract has significant antimicrobial activity against all tested pathogens. However, the best inhibitory effect was observed against Bacillus subtilis ATCC 6633 and Pseudomonas aeruginosa ATCC 90274 with a minimum inhibitory concentration (MIC) of 3.9 μg/ml and minimum bactericidal concentration (MBC) of 15.6 μg/ml, for both pathogens. Also, PsEAE exerts a significant inhibition on the biofilm formation of the previously mentioned pathogenic strains. In addition, we evaluated the wound healing efficiency of PsEAE on earthworms (Lumbricus castaneus) as a feasible and plausible model that mimics human skin. Interestingly, PsEAE exhibited a promising wound healing activity and enhanced wound closure. In conclusion, Paecilomyces sp. (AUMC 15510) could be a sustainable source of antimicrobial agents and a potential therapeutic target for wound management.

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