Jagged-1/Notch Pathway and Key Transient Markers Involved in Biliary Fibrosis During Infection

Overview

Journal Trop Med Infect Dis

Specialty Infectious Diseases

Date 2022 Nov 10

PMID 36355906

Authors

Anna Kovner

Oxana Zaparina

Yaroslav Kapushchak

Galina Minkova

Viatcheslav Mordvinov

Maria Pakharukova

Affiliations

Soon will be listed here.

Abstract

Chronic opisthorchiasis associated with infection is accompanied by severe fibrotic complications. It is of high practical significance to elucidate the mechanisms of hepatic fibrosis in chronic infection dynamics. The goal of the study is to investigate the temporal profile of key markers and the Jagged1/Notch signaling pathway in the implementation of fibrosis in a chronic infection. For the first time, using histological methods and real-time PCR analysis, we demonstrated the activation of the Jagged1/Notch pathway in liver fibrogenesis, including the activation of the and target genes during experimental opisthorchiasis in . Cluster analysis followed by regression analysis of key markers during the infection showed that Jagged1 and Mmp9have the greatest contribution to the development of cholangiofibrosis and periductal fibrosis. Moreover, we detected a significant increase in the number of Jagged1-positive cells in the liver of chronic opisthorchiasis patients compared to that of the control group without infection. The results of the study are extremely informative both in terms of investigation both diverse fibrosis mechanisms as well as potential targets in complex antihelmintic therapy.

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