Transcriptome Analysis of Response Strategy in Hemerocallis Fulva Under Drought Stress

Overview

Journal Genes Genomics

Specialty Genetics

Date 2022 Nov 8

PMID 36348249

Authors

Xiaoteng Cai

Jialin Liu

Fukuan Zhao

Xiaoqin Wang

Affiliations

Soon will be listed here.

Abstract

Background: Hemerocallis fulva is an important ground cover plant widely used in urban greening. The analysis of the molecular mechanism underlying the drought response of H. fulva can lay a foundation for improving its adaptability and expanding its planting area.

Objective: To reveal the drought response mechanisms of H. fulva, identify candidate unigenes associated with drought response, and lay a foundation for further unigenes functional study and drought resistance improvement of H. fulva via genetic engineering.

Methods: RNA was isolated from H. fulva under different experimental conditions. De novo transcriptomic analysis of the samples was performed to screen drought response unigenes. The transcriptional changes of candidate drought response unigenes were verified by quantitative real-time PCR.

Results: The differentially expressed unigenes and their functions were analyzed after H. fulva treated by PEG-simulated drought stress and rewatering. The candidate unigenes, associated with H. fulva drought response, were identified after transcriptome analysis. Then, the transcription level of drought response unigenes of H. fulva under different conditions was further verified. Abscisic acid, protein phosphorylation, sterol biosynthesis and ion transport were involved in drought response with quick restore in H. fulva. The response unigenes, involved in hormone (ABA, JA, CK and GA) signaling pathways, defense response, high light response, karrikin response and leaf shaping, can maintain at changed expression levels even after stress withdraw.

Conclusion: Hemerocallis fulva has unique drought response mechanism. Negative regulation mechanism may play more important roles in drought response of H. fulva. The analysis of candidate unigenes, associated with drought response, lays a foundation for further drought resistance improvement of H. fulva.

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References

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

An F, Liang Y, Li J, Chen X, Han H, Li F . Construction and significance analysis of the MicroRNA expression profile of Hemerocallis fulva at low temperature. Biosci Biotechnol Biochem. 2014; 78(3):378-83. DOI: 10.1080/09168451.2014.878214. View

Baek D, Shin G, Kim M, Shen M, Lee S, Yun D . Histone Deacetylase HDA9 With ABI4 Contributes to Abscisic Acid Homeostasis in Drought Stress Response. Front Plant Sci. 2020; 11:143. PMC: 7052305. DOI: 10.3389/fpls.2020.00143. View

Banerjee A, Roychoudhury A . Abscisic-acid-dependent basic leucine zipper (bZIP) transcription factors in plant abiotic stress. Protoplasma. 2015; 254(1):3-16. DOI: 10.1007/s00709-015-0920-4. View

Bird D, Beisson F, Brigham A, Shin J, Greer S, Jetter R . Characterization of Arabidopsis ABCG11/WBC11, an ATP binding cassette (ABC) transporter that is required for cuticular lipid secretion. Plant J. 2007; 52(3):485-98. DOI: 10.1111/j.1365-313X.2007.03252.x. View

Bolger A, Lohse M, Usadel B . Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014; 30(15):2114-20. PMC: 4103590. DOI: 10.1093/bioinformatics/btu170. View

Cao M, Liu X, Zhang Y, Xue X, Zhou X, Melcher K . An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants. Cell Res. 2013; 23(8):1043-54. PMC: 3731570. DOI: 10.1038/cr.2013.95. View

Chen K, Li G, Bressan R, Song C, Zhu J, Zhao Y . Abscisic acid dynamics, signaling, and functions in plants. J Integr Plant Biol. 2019; 62(1):25-54. DOI: 10.1111/jipb.12899. View

Cuming A, Cho S, Kamisugi Y, Graham H, Quatrano R . Microarray analysis of transcriptional responses to abscisic acid and osmotic, salt, and drought stress in the moss, Physcomitrella patens. New Phytol. 2007; 176(2):275-287. DOI: 10.1111/j.1469-8137.2007.02187.x. View

10.

Du Y, Fu X, Chu Y, Wu P, Liu Y, Ma L . Biosynthesis and the Roles of Plant Sterols in Development and Stress Responses. Int J Mol Sci. 2022; 23(4). PMC: 8875669. DOI: 10.3390/ijms23042332. View

11.

Fancy N, Bahlmann A, Loake G . Nitric oxide function in plant abiotic stress. Plant Cell Environ. 2016; 40(4):462-472. DOI: 10.1111/pce.12707. View

12.

Fujii H, Zhu J . Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stress. Proc Natl Acad Sci U S A. 2009; 106(20):8380-5. PMC: 2688869. DOI: 10.1073/pnas.0903144106. View

13.

Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M, Seki M . AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell. 2005; 17(12):3470-88. PMC: 1315382. DOI: 10.1105/tpc.105.035659. View

14.

Fujita Y, Nakashima K, Yoshida T, Katagiri T, Kidokoro S, Kanamori N . Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant Cell Physiol. 2009; 50(12):2123-32. DOI: 10.1093/pcp/pcp147. View

15.

Ge K, Liu X, Li X, Hu B, Li L . Isolation of an Gene from That Affects ABA Import and Reduces ABA Sensitivity in . Front Plant Sci. 2017; 8:1150. PMC: 5492558. DOI: 10.3389/fpls.2017.01150. View

16.

Gonzalez-Guzman M, Rodriguez L, Lorenzo-Orts L, Pons C, Sarrion-Perdigones A, Fernandez M . Tomato PYR/PYL/RCAR abscisic acid receptors show high expression in root, differential sensitivity to the abscisic acid agonist quinabactin, and the capability to enhance plant drought resistance. J Exp Bot. 2014; 65(15):4451-64. PMC: 4112642. DOI: 10.1093/jxb/eru219. View

17.

Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011; 29(7):644-52. PMC: 3571712. DOI: 10.1038/nbt.1883. View

18.

Gupta A, Rico-Medina A, Cano-Delgado A . The physiology of plant responses to drought. Science. 2020; 368(6488):266-269. DOI: 10.1126/science.aaz7614. View

19.

Hou F, Li S, Wang J, Kang X, Weng Y, Xing G . Identification and validation of reference genes for quantitative real-time PCR studies in long yellow daylily, Hemerocallis citrina Borani. PLoS One. 2017; 12(3):e0174933. PMC: 5376306. DOI: 10.1371/journal.pone.0174933. View

20.

Hsu P, Dubeaux G, Takahashi Y, Schroeder J . Signaling mechanisms in abscisic acid-mediated stomatal closure. Plant J. 2020; 105(2):307-321. PMC: 7902384. DOI: 10.1111/tpj.15067. View