A Novel Multi-Epitope Edible Vaccine Candidate for Newcastle Disease Virus: Approach

Overview

Journal Iran J Biotechnol

Specialty Biotechnology

Date 2022 Nov 7

PMID 36337069

Authors

Atena Mozafari

Jafar Amani

Shahla Shahsavandi

Ali Hatef Salmanian

Affiliations

Soon will be listed here.

Abstract

Background: Newcastle disease, is one of the most important illnesses in the aviculture industry which shows a constant threat. In this case, the vaccine could be considered an important solution to prevent and control this disease. So, the development of a new and more effective vaccine against Newcastle disease is an urgent need. Immune informatics is an important field that provides insight into the experimental procedure and could facilitate the analysis of large amounts of immunological data generated by experimental research and help to design a new vaccine candidate.

Objectives: This study is aimed at bioinformatics to investigate and select the most immunogenic and conserved epitopes derived from F and HN glycoproteins, which play a key role in pathogenesis and immunity. This strategy could cover a wide range of Newcastle disease viruses.

Materials And Method: For expression in both (as an injectable recombinant vaccine candidate) and maize plant (as an edible vaccine candidate) host, two constructs were designed and analyzed separately. Furthermore, the role of LTB as an effective bio-adjuvant for general eliciting of the immune system and simultaneous expressions with those two antigens was evaluated. Hence, here a multimeric recombinant protein with the abbreviation LHN2F from the highly immunogenic part of HN, F and LTB proteins were designed. The synthetic construct was analyzed based on different bioinformatics tools.

Results: The proper immunogenicity and stability of this multimeric fusion protein have been shown by immunoinformatic methods from various servers. To confirm the function of the designed protein, the final molecule was docked to chicken MHC class I using the Pyrex-python 0.8 program. the results of Immune Epitope analysis were confirmed by the docking results between protein and receptor.

Conclusions: ‎The results of structural and immunological computational studies proposed that the protein deduced from this novel construct could act as a vaccine candidate for Newcastle disease virus‎ control and prophylactic.

Citing Articles

Design of a Multi-Epitope Vaccine against the Glycoproteins of Newcastle Disease Virus by Using an Immunoinformatics Approach.

Randriamamisolonirina N, Razafindrafara M, Maminiaina O ACS Omega. 2025; 10(4):4007-4018.

PMID: 39926542 PMC: 11800045. DOI: 10.1021/acsomega.4c09890.

The heterologous expression of novel recombinant protein composed of HN and F moieties of Newcastle disease virus and immunogenicity evaluation in mouse model.

Mozafari A, Rahmani M, Yasini Nasab Y, Shahsavandi S, Jafari M, Salmanian A Iran J Microbiol. 2024; 16(5):655-665.

PMID: 39534294 PMC: 11551657. DOI: 10.18502/ijm.v16i5.16801.

References

Soria-Guerra R, Nieto-Gomez R, Govea-Alonso D, Rosales-Mendoza S . An overview of bioinformatics tools for epitope prediction: implications on vaccine development. J Biomed Inform. 2014; 53:405-14. DOI: 10.1016/j.jbi.2014.11.003. View

Rosales-Mendoza S, Sandez-Robledo C, Banuelos-Hernandez B, Angulo C . Corn-based vaccines: current status and prospects. Planta. 2017; 245(5):875-888. DOI: 10.1007/s00425-017-2680-1. View

Amani J, Salmanian A, Rafati S, Mousavi S . Immunogenic properties of chimeric protein from espA, eae and tir genes of Escherichia coli O157:H7. Vaccine. 2010; 28(42):6923-9. DOI: 10.1016/j.vaccine.2010.07.061. View

Samykannu G, Vijayababu P, Antonyraj C, Perumal P, Narayanan S, Basheer Ahamed S . In Silico Characterization of B Cell and T Cell Epitopes for Subunit Vaccine Design of Salmonella typhi PgtE: A Molecular Dynamics Simulation Approach. J Comput Biol. 2018; 26(2):105-116. DOI: 10.1089/cmb.2018.0010. View

Hagiwara Y, Iwasaki T, Asanuma H, Sato Y, Sata T, Aizawa C . Effects of intranasal administration of cholera toxin (or Escherichia coli heat-labile enterotoxin) B subunits supplemented with a trace amount of the holotoxin on the brain. Vaccine. 2001; 19(13-14):1652-60. DOI: 10.1016/s0264-410x(00)00412-6. View

Lei H, Peng X, Shu H, Zhao D . Intranasal immunization with live recombinant Lactococcus lactis combined with heat-labile toxin B subunit protects chickens from highly pathogenic avian influenza H5N1 virus. J Med Virol. 2014; 87(1):39-44. DOI: 10.1002/jmv.23983. View

Alexander D, Aldous E, Fuller C . The long view: a selective review of 40 years of Newcastle disease research. Avian Pathol. 2012; 41(4):329-35. DOI: 10.1080/03079457.2012.697991. View

Jeshvaghani F, Rahjerdi A, Amani J, Rad I, Jafari M, Salmanian A . Designing and structure evaluation of multi-epitope vaccine against ETEC and EHEC, an in silico approach. Protein Pept Lett. 2015; 23(1):33-42. DOI: 10.2174/0929866522666151026122116. View

Zhao Y, Hammond R . Development of a candidate vaccine for Newcastle disease virus by epitope display in the Cucumber mosaic virus capsid protein. Biotechnol Lett. 2005; 27(6):375-82. DOI: 10.1007/s10529-005-1773-2. View

10.

Khoury C, Meinersmann R . A genetic hybrid of the Campylobacter jejuni flaA gene with LT-B of Escherichia coli and assessment of the efficacy of the hybrid protein as an oral chicken vaccine. Avian Dis. 1995; 39(4):812-20. View

11.

Tarang S, Kesherwani V, LaTendresse B, Lindgren L, Rocha-Sanchez S, Weston M . In silico Design of a Multivalent Vaccine Against Candida albicans. Sci Rep. 2020; 10(1):1066. PMC: 6978452. DOI: 10.1038/s41598-020-57906-x. View

12.

Yusoff K, Nesbit M, McCartney H, Meulemans G, Alexander D, Collins M . Location of neutralizing epitopes on the fusion protein of Newcastle disease virus strain Beaudette C. J Gen Virol. 1989; 70 ( Pt 11):3105-9. DOI: 10.1099/0022-1317-70-11-3105. View

13.

Hu S, Wang T, Liu Y, Meng C, Wang X, Wu Y . Identification of a variable epitope on the Newcastle disease virus hemagglutinin-neuraminidase protein. Vet Microbiol. 2009; 140(1-2):92-7. DOI: 10.1016/j.vetmic.2009.07.029. View

14.

Miles S, Portela M, Cyrklaff M, Ancarola M, Frischknecht F, Duran R . Combining proteomics and bioinformatics to explore novel tegumental antigens as vaccine candidates against Echinococcus granulosus infection. J Cell Biochem. 2019; 120(9):15320-15336. DOI: 10.1002/jcb.28799. View

15.

Fingerut E, Gutter B, Goldway M, Eliahoo D, Pitcovski J . B subunit of E. coli enterotoxin as adjuvant and carrier in oral and skin vaccination. Vet Immunol Immunopathol. 2006; 112(3-4):253-63. DOI: 10.1016/j.vetimm.2006.03.005. View

16.

Iorio R, Syddall R, Sheehan J, Bratt M, Glickman R, Riel A . Neutralization map of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: domains recognized by monoclonal antibodies that prevent receptor recognition. J Virol. 1991; 65(9):4999-5006. PMC: 248963. DOI: 10.1128/JVI.65.9.4999-5006.1991. View

17.

Jawale C, Lee J . Characterization of a Salmonella Typhimurium ghost carrying an adjuvant protein as a vaccine candidate for the protection of chickens against virulent challenge. Avian Pathol. 2014; 43(6):506-13. DOI: 10.1080/03079457.2014.966303. View

18.

Shey R, Ghogomu S, Esoh K, Nebangwa N, Shintouo C, Nongley N . In-silico design of a multi-epitope vaccine candidate against onchocerciasis and related filarial diseases. Sci Rep. 2019; 9(1):4409. PMC: 6416346. DOI: 10.1038/s41598-019-40833-x. View

19.

Ghosh P, Bhakta S, Bhattacharya M, Sharma A, Sharma G, Lee S . A Novel Multi-Epitopic Peptide Vaccine Candidate Against : In-Silico Identification, Design, Cloning and Validation Through Molecular Dynamics. Int J Pept Res Ther. 2021; 27(2):1149-1166. PMC: 7816556. DOI: 10.1007/s10989-020-10157-w. View

20.

Potocnakova L, Bhide M, Borszekova Pulzova L . An Introduction to B-Cell Epitope Mapping and In Silico Epitope Prediction. J Immunol Res. 2017; 2016:6760830. PMC: 5227168. DOI: 10.1155/2016/6760830. View