Repairing Sciatic Nerve Injury with Self-assembling Peptide Nanofiber Scaffold-containing Chitosan Conduit

Overview

Journal Front Neurol

Specialty Neurology

Date 2022 Oct 31

PMID 36313506

Authors

Xuezhen Shen

Feng Qu

Yilun Pei

Simeng Lei

Suhang Xia

Jing Liang

Shan Li

Xun Sun

Liang Liu

Affiliations

Soon will be listed here.

Abstract

Background: An increasing number of nerve guide scaffolds have been used to replace the "gold-standard" autologous nerve graft for repairing peripheral nerve defects, but nerve regeneration is usually far from complete.

Methods: Here, we designed and prepared two functionalized self-assembling peptides (SAP) with the IKVAV and KLT sequences, which were derived from the combination of laminin and VEGF, respectively. Their mixtures were also obtained to combine the effects of neuroprotective and neurotrophic and proangiogenic factors.

Results: The beneficial effect of peptide gels on nerve regeneration was evaluated using Schwann cells (SCs). As a useful intraluminal filling, a three-dimensional (3D) functionalized self-assembling peptide (SAP) nanofiber hydrogel was formed in the hollow lumen of chitosan conduits under physiological conditions. , the combination of the two functionalized SAP gels containing a chitosan nerve conduit significantly accelerated nerve healing and enhanced morphological repair.

Conclusion: Based on the current findings, the combined application of two functionalized SAP gels with chitosan nerve conduit is a promising therapy for the engineering of peripheral nerve regeneration.

Citing Articles

Hydrogels for Peripheral Nerve Repair: Emerging Materials and Therapeutic Applications.

Taisescu O, Dinescu V, Rotaru-Zavaleanu A, Gresita A, Hadjiargyrou M Gels. 2025; 11(2).

PMID: 39996669 PMC: 11855328. DOI: 10.3390/gels11020126.

Commentary: Techniques and graft materials for repairing peripheral nerve defects.

Stocco E, Barbon S, Emmi A, Tiengo C, De Caro R, Macchi V Front Neurol. 2024; 15:1420324.

PMID: 38974681 PMC: 11224285. DOI: 10.3389/fneur.2024.1420324.

Self-Assembling Peptide SCIBIOIII Hydrogel for Three-Dimensional Cell Culture That Promotes Wound Healing in Diabetic Mice.

He L, Lan S, Cheng Q, Luo Z, Lin X Gels. 2023; 9(4).

PMID: 37102877 PMC: 10137493. DOI: 10.3390/gels9040265.

References

Liu X, Wang X, Horii A, Wang X, Qiao L, Zhang S . In vivo studies on angiogenic activity of two designer self-assembling peptide scaffold hydrogels in the chicken embryo chorioallantoic membrane. Nanoscale. 2012; 4(8):2720-7. DOI: 10.1039/c2nr00001f. View

Cattin A, Burden J, Van Emmenis L, Mackenzie F, Hoving J, Garcia Calavia N . Macrophage-Induced Blood Vessels Guide Schwann Cell-Mediated Regeneration of Peripheral Nerves. Cell. 2015; 162(5):1127-39. PMC: 4553238. DOI: 10.1016/j.cell.2015.07.021. View

Bain J, Mackinnon S, Hunter D . Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg. 1989; 83(1):129-38. DOI: 10.1097/00006534-198901000-00024. View

EVANS G . Peripheral nerve injury: a review and approach to tissue engineered constructs. Anat Rec. 2001; 263(4):396-404. DOI: 10.1002/ar.1120. View

Ronchi G, Jager S, Vaegter C, Raimondo S, Giacobini-Robecchi M, Geuna S . Discrepancies in quantitative assessment of normal and regenerated peripheral nerve fibers between light and electron microscopy. J Peripher Nerv Syst. 2014; 19(3):224-33. DOI: 10.1111/jns.12090. View

Biazar E, Khorasani M, Montazeri N, Pourshamsian K, Daliri M, Rezaei M . Types of neural guides and using nanotechnology for peripheral nerve reconstruction. Int J Nanomedicine. 2010; 5:839-52. PMC: 2963930. DOI: 10.2147/IJN.S11883. View

Du J, Liu J, Yao S, Mao H, Peng J, Sun X . Prompt peripheral nerve regeneration induced by a hierarchically aligned fibrin nanofiber hydrogel. Acta Biomater. 2017; 55:296-309. DOI: 10.1016/j.actbio.2017.04.010. View

Zhan X, Gao M, Jiang Y, Zhang W, Wong W, Yuan Q . Nanofiber scaffolds facilitate functional regeneration of peripheral nerve injury. Nanomedicine. 2012; 9(3):305-15. DOI: 10.1016/j.nano.2012.08.009. View

Hsu S, Kuo W, Chen Y, Yen C, Chen Y, Chen K . New nerve regeneration strategy combining laminin-coated chitosan conduits and stem cell therapy. Acta Biomater. 2013; 9(5):6606-15. DOI: 10.1016/j.actbio.2013.01.025. View

10.

Kim J, Bax D, Murphy R, Best S, Cameron R . Collagen Film Activation with Nanoscale IKVAV-Capped Dendrimers for Selective Neural Cell Response. Nanomaterials (Basel). 2021; 11(5). PMC: 8146934. DOI: 10.3390/nano11051157. View

11.

Burnett M, Zager E . Pathophysiology of peripheral nerve injury: a brief review. Neurosurg Focus. 2004; 16(5):E1. DOI: 10.3171/foc.2004.16.5.2. View

12.

Zhou Y, Liu S, Zhao M, Wang C, Li L, Yuan Y . Injectable extracellular vesicle-released self-assembling peptide nanofiber hydrogel as an enhanced cell-free therapy for tissue regeneration. J Control Release. 2019; 316:93-104. DOI: 10.1016/j.jconrel.2019.11.003. View

13.

Lampe K, Heilshorn S . Building stem cell niches from the molecule up through engineered peptide materials. Neurosci Lett. 2012; 519(2):138-46. PMC: 3691058. DOI: 10.1016/j.neulet.2012.01.042. View

14.

Lu J, Yan X, Sun X, Shen X, Yin H, Wang C . Synergistic effects of dual-presenting VEGF- and BDNF-mimetic peptide epitopes from self-assembling peptide hydrogels on peripheral nerve regeneration. Nanoscale. 2019; 11(42):19943-19958. DOI: 10.1039/c9nr04521j. View

15.

Sanchez de Groot N, Parella T, Aviles F, Vendrell J, Ventura S . Ile-phe dipeptide self-assembly: clues to amyloid formation. Biophys J. 2006; 92(5):1732-41. PMC: 1796831. DOI: 10.1529/biophysj.106.096677. View

16.

Hong T, Tian A, Sachar R, Ray W, Brogan D, Dy C . Indirect Cost of Traumatic Brachial Plexus Injuries in the United States. J Bone Joint Surg Am. 2019; 101(16):e80. PMC: 7406142. DOI: 10.2106/JBJS.18.00658. View

17.

Takagi Y, Nomizu M, Gullberg D, MacKrell A, Keene D, Yamada Y . Conserved neuron promoting activity in Drosophila and vertebrate laminin alpha1. J Biol Chem. 1996; 271(30):18074-81. DOI: 10.1074/jbc.271.30.18074. View

18.

Wang Y, Zhao Y, Sun C, Hu W, Zhao J, Li G . Chitosan Degradation Products Promote Nerve Regeneration by Stimulating Schwann Cell Proliferation via miR-27a/FOXO1 Axis. Mol Neurobiol. 2014; 53(1):28-39. DOI: 10.1007/s12035-014-8968-2. View

19.

Campbell W . Evaluation and management of peripheral nerve injury. Clin Neurophysiol. 2008; 119(9):1951-65. DOI: 10.1016/j.clinph.2008.03.018. View

20.

Noble J, Munro C, Prasad V, Midha R . Analysis of upper and lower extremity peripheral nerve injuries in a population of patients with multiple injuries. J Trauma. 1998; 45(1):116-22. DOI: 10.1097/00005373-199807000-00025. View