Combined Oral Immunization with Probiotics Entercoccus Faecalis Delivering Surface-anchored Proteins Provide Protective Efficacies Against Homologous Infection in Chickens

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2022 Oct 31

PMID 36311704

Authors

Wenjing Zhi

Hang Chen

Bingrong Bai

Zhipeng Jia

Xinghui Pan

Biao Wang

Rui Kong

Qiuju Liu

Chunli Ma

Dexing Ma

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: Avian coccidiosis is an intestinal parasitic disease exerting a highly negative impact on the global poultry industry. The aim of the present study is to evaluate the immune protective efficacies against infection in chickens orally immunized with combined recombinant probiotics delivering surface-anchored proteins.

Methods: Four kinds of novel probiotics vaccines that surfaceexpressing four () proteins EtAMA1, EtIMP1, EtMIC2 and Et3-1E were produced, respectively. The expression of four target proteins on the surface of recombinant bacteria was detected by Western blot and indirect immunofluorescence assay (IFA). Then the four kinds of recombinant were combined to immunize chickens oral route in different combinations. The immunizations were performed three times at two-week intervals, and each for three consecutive days. After immunizations, chickens in each immunized group were orally challenged with sporulated oocysts. The immune responses and protective efficacies against homologous infection were evaluated.

Results: The results showed that three or four live recombinant induced effective antigen-specific humoral, intestinal mucosal immune responses, stimulated peripheral T lymphocytes proliferation, and displayed partial protections against homologous challenge as measured by cecal lesions, oocyst shedding, and body weight gain (BWG). Notably, higher levels of protective efficacies were observed when the four recombinant delivering target proteins were combined.

Conclusion: Chickens orally administrated with three or four, especially the four combined recombinant stimulated specific immune responses, which provided anti-coccidial effects. This study offers an idea for future development of novel vaccines based on multi-antigens delivered by probiotic bacteria.

Citing Articles

What Do We Know about Surface Proteins of Chicken Parasites ?.

Britez J, Rodriguez A, Di Ciaccio L, Marugan-Hernandez V, Tomazic M Life (Basel). 2023; 13(6).

PMID: 37374079 PMC: 10301564. DOI: 10.3390/life13061295.

References

Lopez-Osorio S, Chaparro-Gutierrez J, Gomez-Osorio L . Overview of Poultry Life Cycle and Host-Parasite Interactions. Front Vet Sci. 2020; 7:384. PMC: 7351014. DOI: 10.3389/fvets.2020.00384. View

Piard J, Hautefort I, Fischetti V, Ehrlich S, Fons M, Gruss A . Cell wall anchoring of the Streptococcus pyogenes M6 protein in various lactic acid bacteria. J Bacteriol. 1997; 179(9):3068-72. PMC: 179078. DOI: 10.1128/jb.179.9.3068-3072.1997. View

Huang H, Jiang Y, Zhou F, Shi C, Yang W, Wang J . A potential vaccine candidate towards chicken coccidiosis mediated by recombinant Lactobacillus plantarum with surface displayed EtMIC2 protein. Exp Parasitol. 2020; 215:107901. DOI: 10.1016/j.exppara.2020.107901. View

Yu Z, Chen S, Huang J, Ding W, Chen Y, Su J . A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens. Vet Res. 2022; 53(1):27. PMC: 9350682. DOI: 10.1186/s13567-022-01045-w. View

Jiang L, Lin J, Han H, Dong H, Zhao Q, Zhu S . Identification and characterization of Eimeria tenella apical membrane antigen-1 (AMA1). PLoS One. 2012; 7(7):e41115. PMC: 3400601. DOI: 10.1371/journal.pone.0041115. View

Li J, Wang F, Ma C, Huang Y, Wang D, Ma D . Recombinant lactococcus lactis expressing Eimeria tenella AMA1 protein and its immunological effects against homologous challenge. Exp Parasitol. 2018; 191:1-8. DOI: 10.1016/j.exppara.2018.05.003. View

Kong W, Wang X, Fields E, Okon B, Jenkins M, Wilkins G . Mucosal Delivery of a Self-destructing Salmonella-Based Vaccine Inducing Immunity Against Eimeria. Avian Dis. 2020; 64(3):254-268. DOI: 10.1637/aviandiseases-D-19-00159. View

Johnson J, REID W . Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol. 1970; 28(1):30-6. DOI: 10.1016/0014-4894(70)90063-9. View

Song X, Gao Y, Xu L, Yan R, Li X . Partial protection against four species of chicken coccidia induced by multivalent subunit vaccine. Vet Parasitol. 2015; 212(3-4):80-5. DOI: 10.1016/j.vetpar.2015.08.026. View

10.

Jauregui-Zuniga D, Pedraza-Escalona M, Espino-Solis G, Quintero-Hernandez V, Olvera-Rodriguez A, Diaz-Salinas M . Targeting antigens to Dec-205 on dendritic cells induces a higher immune response in chickens: Hemagglutinin of avian influenza virus example. Res Vet Sci. 2016; 111:55-62. DOI: 10.1016/j.rvsc.2016.12.002. View

11.

Zhao N, Lv J, Lu Y, Jiang Y, Li H, Liu Y . Prolonging and enhancing the protective efficacy of the EtMIC3-C-MAR against eimeria tenella through delivered by attenuated salmonella typhimurium. Vet Parasitol. 2020; 279:109061. DOI: 10.1016/j.vetpar.2020.109061. View

12.

Hong Y, Lillehoj H, Lee S, Dalloul R, Lillehoj E . Analysis of chicken cytokine and chemokine gene expression following Eimeria acervulina and Eimeria tenella infections. Vet Immunol Immunopathol. 2006; 114(3-4):209-23. DOI: 10.1016/j.vetimm.2006.07.007. View

13.

Pham H, Matsubayashi M, Tsuji N, Hatabu T . Relationship between Eimeria tenella associated-early clinical signs and molecular changes in the intestinal barrier function. Vet Immunol Immunopathol. 2021; 240:110321. DOI: 10.1016/j.vetimm.2021.110321. View

14.

Ma C, Zhang L, Gao M, Ma D . Construction of Lactococcus lactis expressing secreted and anchored Eimeria tenella 3-1E protein and comparison of protective immunity against homologous challenge. Exp Parasitol. 2017; 178:14-20. DOI: 10.1016/j.exppara.2017.05.001. View

15.

Singh D, Singh M, Chander V, Sharma G, Mahawar M, Teeli A . Differential responses of chicken monocyte-derived dendritic cells infected with Salmonella Gallinarum and Salmonella Typhimurium. Sci Rep. 2021; 11(1):17214. PMC: 8390485. DOI: 10.1038/s41598-021-96527-w. View

16.

Yang G, Yao J, Yang W, Jiang Y, Du J, Huang H . Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing SO7 of Eimeria tenella fusion DC-targeting peptide. Vet Parasitol. 2017; 236:7-13. DOI: 10.1016/j.vetpar.2017.01.023. View

17.

Pastor-Fernandez I, Kim S, Marugan-Hernandez V, Soutter F, Tomley F, Blake D . Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis. Parasit Vectors. 2020; 13(1):343. PMC: 7350274. DOI: 10.1186/s13071-020-04210-2. View

18.

Yin G, Lin Q, Qiu J, Qin M, Tang X, Suo X . Immunogenicity and protective efficacy of an Eimeria vaccine candidate based on Eimeria tenella immune mapped protein 1 and chicken CD40 ligand. Vet Parasitol. 2015; 210(1-2):19-24. DOI: 10.1016/j.vetpar.2015.03.012. View

19.

Tang X, Suo J, Li C, Du M, Wang C, Hu D . Transgenic Eimeria tenella Expressing Profilin of Eimeria maxima Elicits Enhanced Protective Immunity and Alters Gut Microbiome of Chickens. Infect Immun. 2018; 86(9). PMC: 6105905. DOI: 10.1128/IAI.00888-17. View

20.

Chen Z, Wang X, Zhao N, Han L, Wang F, Li H . Improving the immunogenicity and protective efficacy of the EtMIC2 protein against Eimeria tenella infection through random mutagenesis. Vaccine. 2018; 36(18):2435-2441. DOI: 10.1016/j.vaccine.2018.03.046. View