PpZAT5 Suppresses the Expression of a B-box Gene to Inhibit Anthocyanin Biosynthesis in the Fruit Peel of Red Pear

Overview

Journal Front Plant Sci

Date 2022 Oct 28

PMID 36304405

Authors

Lu Zhang

Ruiyan Tao

Simai Wang

Yuhao Gao

Lu Wang

Shulin Yang

Xiao Zhang

Wenjie Yu

Xinyue Wu

Kunfeng Li

Junbei Ni

Yuanwen Teng

Songling Bai

Affiliations

Soon will be listed here.

Abstract

BBX (B-box) proteins play a vital role in light-induced anthocyanin biosynthesis. PpBBX18 was an indispensable regulator for the induction of anthocyanin biosynthesis in the peel of red pear fruit ( Nakai.). However, the upstream regulation of BBX genes has not been well characterized. In this study, PpZAT5, a cysteine2/histidine2-type transcription factor, was discovered as the upstream negative regulator of PpBBX18. The results showed that PpZAT5 functions as a transcriptional repressor and directly binds to the CAAT motif of and inhibits its expression. expression was inhibited by light, which is converse to the expression pattern of anthocyanin-related structural genes. In addition, less anthocyanin accumulated in the overexpressing pear calli than in the wild-type pear calli; on the contrary, more anthocyanin accumulated in PpZAT5-RNAi pear calli. Moreover, the crucial genes involved in light-induced anthocyanin biosynthesis were markedly down-regulated in the transcriptome of PpZAT5 overexpression pear calli compared to wild-type. In conclusion, our study indicates that is negatively regulated by a C2H2-type transcriptional repressor, PpZAT5, which reduces anthocyanin content in pear. The present results demonstrate an upstream molecular mechanism of and provide insights into light-induced anthocyanin biosynthesis.

Citing Articles

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References

Vogel J, Zarka D, Van Buskirk H, Fowler S, Thomashow M . Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. Plant J. 2005; 41(2):195-211. DOI: 10.1111/j.1365-313X.2004.02288.x. View

Chang C, Li Y, Chen L, Chen W, Hsieh W, Shin J . LZF1, a HY5-regulated transcriptional factor, functions in Arabidopsis de-etiolation. Plant J. 2008; 54(2):205-19. DOI: 10.1111/j.1365-313X.2008.03401.x. View

Ni J, Premathilake A, Gao Y, Yu W, Tao R, Teng Y . Ethylene-activated PpERF105 induces the expression of the repressor-type R2R3-MYB gene PpMYB140 to inhibit anthocyanin biosynthesis in red pear fruit. Plant J. 2020; 105(1):167-181. DOI: 10.1111/tpj.15049. View

Borevitz J, Xia Y, Blount J, Dixon R, Lamb C . Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell. 2001; 12(12):2383-2394. PMC: 102225. DOI: 10.1105/tpc.12.12.2383. View

Kagale S, Links M, Rozwadowski K . Genome-wide analysis of ethylene-responsive element binding factor-associated amphiphilic repression motif-containing transcriptional regulators in Arabidopsis. Plant Physiol. 2010; 152(3):1109-34. PMC: 2832246. DOI: 10.1104/pp.109.151704. View

Takos A, Jaffe F, Jacob S, Bogs J, Robinson S, Walker A . Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiol. 2006; 142(3):1216-32. PMC: 1630764. DOI: 10.1104/pp.106.088104. View

. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol. 2001; 126(2):485-93. PMC: 1540115. DOI: 10.1104/pp.126.2.485. View

Ohta M, Matsui K, Hiratsu K, Shinshi H, Ohme-Takagi M . Repression domains of class II ERF transcriptional repressors share an essential motif for active repression. Plant Cell. 2001; 13(8):1959-68. PMC: 139139. DOI: 10.1105/tpc.010127. View

Espley R, Hellens R, Putterill J, Stevenson D, Kutty-Amma S, Allan A . Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. Plant J. 2006; 49(3):414-27. PMC: 1865000. DOI: 10.1111/j.1365-313X.2006.02964.x. View

10.

Liu W, Zhang F, Zhang W, Song L, Wu W, Chen Y . Arabidopsis Di19 functions as a transcription factor and modulates PR1, PR2, and PR5 expression in response to drought stress. Mol Plant. 2013; 6(5):1487-502. DOI: 10.1093/mp/sst031. View

11.

Pertea M, Kim D, Pertea G, Leek J, Salzberg S . Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown. Nat Protoc. 2016; 11(9):1650-67. PMC: 5032908. DOI: 10.1038/nprot.2016.095. View

12.

Ban Y, Honda C, Hatsuyama Y, Igarashi M, Bessho H, Moriguchi T . Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant Cell Physiol. 2007; 48(7):958-70. DOI: 10.1093/pcp/pcm066. View

13.

Takatsuji H, Mori M, Benfey P, Ren L, Chua N . Characterization of a zinc finger DNA-binding protein expressed specifically in Petunia petals and seedlings. EMBO J. 1992; 11(1):241-9. PMC: 556445. DOI: 10.1002/j.1460-2075.1992.tb05047.x. View

14.

Xu D, Jiang Y, Li J, Lin F, Holm M, Deng X . BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradation. Proc Natl Acad Sci U S A. 2016; 113(27):7655-60. PMC: 4941485. DOI: 10.1073/pnas.1607687113. View

15.

Ciftci-Yilmaz S, Mittler R . The zinc finger network of plants. Cell Mol Life Sci. 2008; 65(7-8):1150-60. PMC: 11131624. DOI: 10.1007/s00018-007-7473-4. View

16.

Ni J, Zhao Y, Tao R, Yin L, Gao L, Strid A . Ethylene mediates the branching of the jasmonate-induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits. Plant Biotechnol J. 2019; 18(5):1223-1240. PMC: 7152598. DOI: 10.1111/pbi.13287. View

17.

Job N, Yadukrishnan P, Bursch K, Datta S, Johansson H . Two B-Box Proteins Regulate Photomorphogenesis by Oppositely Modulating HY5 through their Diverse C-Terminal Domains. Plant Physiol. 2018; 176(4):2963-2976. PMC: 5884587. DOI: 10.1104/pp.17.00856. View

18.

Gangappa S, Botto J . The Multifaceted Roles of HY5 in Plant Growth and Development. Mol Plant. 2016; 9(10):1353-1365. DOI: 10.1016/j.molp.2016.07.002. View

19.

Lee H, Guo Y, Ohta M, Xiong L, Stevenson B, Zhu J . LOS2, a genetic locus required for cold-responsive gene transcription encodes a bi-functional enolase. EMBO J. 2002; 21(11):2692-702. PMC: 126021. DOI: 10.1093/emboj/21.11.2692. View

20.

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View